dsbB:Gene

From EcoliWiki
Jump to: navigation, search

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

<protect>

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dsbB

Mnemonic

Disulfide bond

Synonyms

ECK1173, b1185, JW5182, iarB, roxB, dsbX, ycgA, rosB[1][2]

</protect>

Notes

Location(s) and DNA Sequence

[back to top]


<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

26.55 minutes 

MG1655: 1232253..1231723
<gbrowseImage> name=NC_000913:1231723..1232253 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1233180..1232650
<gbrowseImage> name=NC_012967:1232650..1233180 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1120444..1120974
<gbrowseImage> name=NC_012759:1120444..1120974 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1234607..1234077
<gbrowseImage> name=NC_007779:1234077..1234607 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1272494..1271964
<gbrowseImage> name=NC_010473:1271964..1272494 source=DH10B preset=GeneLocation </gbrowseImage>

</protect>

Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

<protect></protect>

Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

dsbB(del) (Keio:JW5182)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8325[4]

dsbB2::kan

PMID:8917542[5] PMID:9352906[6]

CGSC:65867

dsbB774(del)::kan

PMID:16738554[3]

CGSC:100650

dsbB65::Tn10

insertion

Disulfide Bond formation

Defective Disulfide Bond formation

PMID:8168498[7]

The mutation caused a Defect in Disulfide Bond formation. See figure 2 for experimental results.

<protect></protect>

Notes

Genetic Interactions

[back to top]


<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

</protect>

Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5182

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCTTGCGATTTTTGAACCAATG

Primer 2:CCGCGACCGAACAGATCACGTTT

2A3

Kohara Phage

Genobase

PMID:3038334[9]

11G8

Kohara Phage

Genobase

PMID:3038334[9]

zcf-117::Tn10

Linked marker

CAG18463 = CGSC7363[4]

est. P1 cotransduction: 47% [10]

fadR13::Tn10

Linked marker

CAG18497 = CGSC7365[4]

est. P1 cotransduction: 92% [10]

<protect></protect>

Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11393

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11393

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001360

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946344

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1366

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003979

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

[back to top]



<protect>

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Rietsch, A et al. (1996) An in vivo pathway for disulfide bond isomerization in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 93 13048-53 PubMed
  6. Rietsch, A et al. (1997) Reduction of the periplasmic disulfide bond isomerase, DsbC, occurs by passage of electrons from cytoplasmic thioredoxin. J. Bacteriol. 179 6602-8 PubMed
  7. Missiakas, D et al. (1994) The Escherichia coli dsbC (xprA) gene encodes a periplasmic protein involved in disulfide bond formation. EMBO J. 13 2013-20 PubMed
  8. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  10. 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories