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Standard Name	dpiB
Gene Synonym(s)	citA, ECK0612, b0619, JW0611, ybeP, cit, mpdB^[1]^[2], mpdB
Product Desc.	sensory histidine kinase in two-component regulatory system with CitB^[3]; sensory histidine kinase in two-component regulatory system with DpiA^[2] Two-component system histidine protein kinase; DpiA is cognate sensor; regulates citCDEFXGT operon; represses appY; periplasmic domain functions as a high-affinity citrate receptor; destabilizes plasmid inheritance^[4]
Product Synonyms(s)	sensory histidine kinase in two-component regulatory system with citB^[1], B0619^[3]^[1], MpdB^[3]^[1], YbeP^[3]^[1], DpiB^[3]^[1], CitA^[3]^[1] , citA, ECK0612, JW0611, mpdB, ybeP, b0619
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): citA^[3]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	dpiB
Mnemonic	Destabilizer of plasmid inheritance
Synonyms	citA, ECK0612, b0619, JW0611, ybeP, cit, mpdB^[1]^[2], mpdB
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	14.04 minutes	MG1655: 651458..653116 <gbrowseImage> name=NC_000913:651458..653116 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 634823..636481 <gbrowseImage> name=NC_012967:634823..636481 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 554218..555876 <gbrowseImage> name=NC_012759:554218..555876 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 651458..653116 <gbrowseImage> name=NC_007779:651458..653116 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔcitA757::kan					PMID:16738554	CGSC:100316
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
	Plasmid clone	Shigen	PMID:16769691 Status: Primer 1: Primer 2:
3G5	Kohara Phage	Genobase	PMID:3038334
1G6	Kohara Phage	Genobase	PMID:3038334
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6345	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13646	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002952	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945233	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3410	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002129	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	CitA
Synonyms	sensory histidine kinase in two-component regulatory system with citB^[1], B0619^[3]^[1], MpdB^[3]^[1], YbeP^[3]^[1], DpiB^[3]^[1], CitA^[3]^[1] , citA, ECK0612, JW0611, mpdB, ybeP, b0619
Product description	sensory histidine kinase in two-component regulatory system with CitB^[3]; sensory histidine kinase in two-component regulatory system with DpiA^[2] Two-component system histidine protein kinase; DpiA is cognate sensor; regulates citCDEFXGT operon; represses appY; periplasmic domain functions as a high-affinity citrate receptor; destabilizes plasmid inheritance^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000155	two-component sensor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005467	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000155	two-component sensor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016120	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000160	two-component signal transduction system (phosphorelay)	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016120	P	Seeded from EcoCyc (v14.0)	complete
	GO:0000160	two-component signal transduction system (phosphorelay)	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0902	P	Seeded from EcoCyc (v14.0)	complete
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003594	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006355	regulation of transcription, DNA-dependent	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013767	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	PMID:19202292			C	Seeded from EcoCyc (v14.0)	Missing: evidence
	GO:0018106	peptidyl-histidine phosphorylation	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005467	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	groL	PMID:16606699	Experiment(s):EBI-1137450
Protein	glpD	PMID:16606699	Experiment(s):EBI-1137450
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane	C-terminus localized in the cytoplasm with 2 predicted transmembrane domains	Daley et al. (2005) ^[5]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MLQLNENKQF AFFQRLAFPL RIFLLILVFS IFVIAALAQY FTASFEDYLT LHVRDMAMNQ AKIIASNDSV ISAVKTRDYK RLATIANKLQ RDTDFDYVVI GDRHSIRLYH PNPEKIGYPM QFTKQGALEK GESYFITGKG SMGMAMRAKT PIFDDDGKVI GVVSIGYLVS KIDSWRAEFL LPMAGVFVVL LGILMLLSWF LAAHIRRQMM GMEPKQIARV VRQQEALFSS VYEGLIAVDP HGYITAINRN ARKMLGLSSP GRQWLGKPIV EVVRPADFFT EQIDEKRQDV VANFNGLSVI ANREAIRSGD DLLGAIISFR SKDEISTLNA QLTQIKQYVE SLRTLRHEHL NWMSTLNGLL QMKEYDRVLA MVQGESQAQQ QLIDSLREAF ADRQVAGLLF GKVQRARELG LKMIIVPGSQ LSQLPPGLDS TEFAAIVGNL LDNAFEASLR SDEGNKIVEL FLSDEGDDVV IEVADQGCGV PESLRDKIFE QGVSTRADEP GEHGIGLYLI ASYVTRCGGV ITLEDNDPCG TLFSIYIPKV KPNDSSINPI DR
Length	552
Mol. Wt	61.685 kDa
pI	6.0 (calculated)
Extinction coefficient	44,350 - 44,725 (calc based on 15 Y, 4 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	428..540	PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase	PMID:19920124
Domain	222..293	PF00989 PAS fold	PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=dpiB taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128602	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945233	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002129	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P77510	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6345	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13646	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945233	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002952	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3410	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	4^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	2^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
citA
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File:.jpg

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:651438..651478 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0619 (EcoliWiki Page)	NCBI GEO profiles for dpiB
microarray	GenExpDB:b0619 (EcoliWiki Page)	Summary of data for dpiB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (651091..651500) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ06; Well:G6^[6]
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Notes

Accessions Related to dpiB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6345	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3410	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0619
EcoGene	EcoGene:EG13646	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002952	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002129	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02518 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Superfamily (EcoliWiki Page)	SUPERFAMILY:55785
Superfamily (EcoliWiki Page)	SUPERFAMILY:55874
Superfamily (EcoliWiki Page)	SUPERFAMILY:55890
Superfamily (EcoliWiki Page)	SUPERFAMILY:103190
Pfam (EcoliWiki Page)	PF00989 PAS fold
EcoCyc	EcoCyc:G6345	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13646	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002952	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3410	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002129	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.00 ^3.01 ^3.02 ^3.03 ^3.04 ^3.05 ^3.06 ^3.07 ^3.08 ^3.09 ^3.10 ^3.11 ^3.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

dpiB:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

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Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to dpiB Expression

Notes

Links

Evolution

Homologs in Other Organisms

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Notes

Families

Notes

Links

References

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