dosP:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
dosP |
|---|---|
| Mnemonic | |
| Synonyms |
ECK1483, b1489, JW1484, yddU, dos[1], dos, yddU |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
33.65 minutes |
MG1655: 1563757..1561358 |
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NC_012967: 1539671..1537272 |
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NC_012759: 1453417..1455816 |
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W3110 |
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W3110: 1567447..1565048 |
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DH10B: 1654352..1651953 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
Δdos (Keio:JW1484) |
deletion |
deletion |
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|
dosH582A |
H582A |
Loss of cAMP PDE activity |
seeded from UniProt:P76129 | ||||
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dosH586A |
H586A |
Loss of cAMP PDE activity |
seeded from UniProt:P76129 | ||||
|
dosH69A,G |
H69A,G |
Loss of heme binding |
seeded from UniProt:P76129 | ||||
|
dosH75A,G |
H75A,G |
No loss of heme binding |
seeded from UniProt:P76129 | ||||
|
dosL91T |
L91T |
Increases auto-oxidation |
seeded from UniProt:P76129 | ||||
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dosL107F |
L107F |
Significantly reduces heme-binding affinity; increases auto-oxidation |
seeded from UniProt:P76129 | ||||
|
dosL107T |
L107T |
Increases auto-oxidation |
seeded from UniProt:P76129 | ||||
|
dosM87A,I |
M87A,I |
Ferrous heme iron changes from an exclusively hexacoordinate low-spin form to an exclusively pentacoordinate high- spin form. Ferric heme iron remains hexacoordinate but becomes a mixture of high and low spin. Increases c-di-GMP PDE activity 7-fold in absence of O(2), CO or NO, no additional increase upon addition of gases (M-A only) |
seeded from UniProt:P76129 | ||||
|
dosM87H |
M87H |
No change in heme coordination; increases c-di-GMP PDE activity 2-fold in absence of O(2), CO or NO, and 2-fold more upon addition of gases |
seeded from UniProt:P76129 | ||||
|
dosR89A,E,I |
R89A,E,I |
The Fe(2+)-O(2) form loses c- di-GMP PDE activity, due to reduced O(2) affinity and/or increased auto-oxidation. NO and CO forms are less affected |
seeded from UniProt:P76129 | ||||
|
dosL91F |
L91F |
Alters O(2) binding, increases auto-oxidation |
seeded from UniProt:P76129 | ||||
|
Δdos-782::kan |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW1484 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAAGCTAACCGATGCGGATAA Primer 2:CCGATTTTCAGCGGTAACACGCT | |
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Kohara Phage |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 38% [6] | ||
|
zdi-925::Tn10 |
Linked marker |
est. P1 cotransduction: % [6] | |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
RegulonDB: |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 5.0 5.1 5.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
