{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Standard Name	dhaM
Gene Synonym(s)	dhaH, ECK1186, b1198, JW5185, ycgC, ptsD^[1]^[2], ptsD
Product Desc.	dihydroxyacetone kinase subunit M^[2]^[3]; Component of dihydroxyacetone kinase^[3] Multidomain dihydroxyacetone-specific PTS protein; phosphoryl donor for dihydroxyacetone kinase; DhaK(2):DhaL:DhaM(2) complex^[4]
Product Synonyms(s)	fused predicted dihydroxyacetone-specific PTS enzymes: HPr component^[1], EI component^[1], DhaM^[2]^[1], B1198^[2]^[1], DhaH^[2]^[1], YcgC^[2]^[1], PtsD^[2]^[1] , dhaH, ECK1186, JW5185, ptsD, ycgC, b1198
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): dhaKLM^[2]
Regulation/Activity
Quick Links
edit table

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

[back to top]

DhaM is phosphorylated by PTS proteins HPr and Protein I. DhaM contains homology to Hpr and part of Enzyme I. DhaM lacks a homologous PEP binding site and catalytic region of HPr. The N-terminal dimerization domain is homologous to an EIIa domain and is the phosphate donor region. H431 accepts phosphate from Hpr and relays it to H10 and H170.^[4]

Gene

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css> <protect>

Nomenclature

[back to top]

See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	dhaM
Mnemonic	Dihydroxyacetone
Synonyms	dhaH, ECK1186, b1198, JW5185, ycgC, ptsD^[1]^[2], ptsD
edit table

</protect>

Notes

Location(s) and DNA Sequence

[back to top]

<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	26.88 minutes	MG1655: 1248337..1246919 <gbrowseImage> name=NC_000913:1246919..1248337 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1249264..1247846 <gbrowseImage> name=NC_012967:1247846..1249264 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1135640..1137061 <gbrowseImage> name=NC_012759:1135640..1137061 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1250691..1249273 <gbrowseImage> name=NC_007779:1249273..1250691 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1288578..1287160 <gbrowseImage> name=NC_010473:1287160..1288578 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
edit table

</protect>

Notes

Sequence Features

[back to top]

See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
edit table

Notes

Alleles and Phenotypes

[back to top]

See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	AA change(s)	Phenotype: Description	Reference	Availability	Comments
dhaMH430A	H430A	Loss of activity			seeded from UniProt:P37349
dhaMH169A	H169A	Loss of activity			seeded from UniProt:P37349
dhaMH9A	H9A	Loss of activity			seeded from UniProt:P37349
ΔdhaM788::kan			PMID:16738554	CGSC:106158
edit table

Notes

Genetic Interactions

[back to top]

Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
edit table

</protect>

Notes

Genetic Resources

[back to top]

See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
	Plasmid clone	Shigen	PMID:16769691 Status: Primer 1: Primer 2:
7C10	Kohara Phage	Genobase	PMID:3038334
12A3	Kohara Phage	Genobase	PMID:3038334
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12399	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12399	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002261	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945749	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2299	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004024	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Product(s)

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	DhaH
Synonyms	fused predicted dihydroxyacetone-specific PTS enzymes: HPr component^[1], EI component^[1], DhaM^[2]^[1], B1198^[2]^[1], DhaH^[2]^[1], YcgC^[2]^[1], PtsD^[2]^[1] , dhaH, ECK1186, JW5185, ptsD, ycgC, b1198
Product description	dihydroxyacetone kinase subunit M^[2]^[3]; Component of dihydroxyacetone kinase^[3] Multidomain dihydroxyacetone-specific PTS protein; phosphoryl donor for dihydroxyacetone kinase; DhaK(2):DhaL:DhaM(2) complex^[4]
EC number (for enzymes)
edit table

Notes

Function

[back to top]

<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005351	sugar:hydrogen symporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000032	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005351	sugar:hydrogen symporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001020	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005351	sugar:hydrogen symporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005698	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005351	sugar:hydrogen symporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008731	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008731	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006071	glycerol metabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0319	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008965	phosphoenolpyruvate-protein phosphotransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008731	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009401	phosphoenolpyruvate-dependent sugar phosphotransferase system	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000032	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009401	phosphoenolpyruvate-dependent sugar phosphotransferase system	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001020	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009401	phosphoenolpyruvate-dependent sugar phosphotransferase system	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004701	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009401	phosphoenolpyruvate-dependent sugar phosphotransferase system	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005698	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009401	phosphoenolpyruvate-dependent sugar phosphotransferase system	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008731	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009401	phosphoenolpyruvate-dependent sugar phosphotransferase system	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0598	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004701	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016301	kinase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0418	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016310	phosphorylation	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008279	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016772	transferase activity, transferring phosphorus-containing groups	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008279	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006974	response to DNA damage stimulus	PMID:11967071	IEP: Inferred from Expression Pattern		P		complete
edit table

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of dihydroxyacetone kinase	could be indirect
Protein	maeB		PMID:15690043	Experiment(s):EBI-890069
Protein	dnaK		PMID:15690043	Experiment(s):EBI-890069
Protein	stfR		PMID:15690043	Experiment(s):EBI-895025
Protein	cysP		PMID:15690043	Experiment(s):EBI-895025
Protein	yjeH		PMID:15690043	Experiment(s):EBI-895025
Protein	maeB		PMID:19402753	MALDI(Z-score):27.693192
Protein	stfR		PMID:19402753	LCMS(ID Probability):99.6
Protein	yjeH		PMID:19402753	LCMS(ID Probability):99.6
Protein	cysP		PMID:19402753	LCMS(ID Probability):99.6
edit table

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
edit table

Notes

Structure and Physical Properties

[back to top]

<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MMVNLVIVSH SSRLGEGVGE LARQMLMSDS CKIAIAAGID DPQNPIGTDA VKVMEAIESV ADADHVLVMM DMGSALLSAE TALELLAPEI AAKVRLCAAP LVEGTLAATV SAASGADIDK VIFDAMHALE AKREQLGLPS SDTEISDTCP AYDEEARSLA VVIKNRNGLH VRPASRLVYT LSTFNADMLL EKNGKCVTPE SINQIALLQV RYNDTLRLIA KGPEAEEALI AFRQLAEDNF GETEEVAPPT LRPVPPVSGK AFYYQPVLCT VQAKSTLTVE EEQDRLRQAI DFTLLDLMTL TAKAEASGLD DIAAIFSGHH TLLDDPELLA AASELLQHEH CTAEYAWQQV LKELSQQYQQ LDDEYLQARY IDVDDLLHRT LVHLTQTKEE LPQFNSPTIL LAENIYPSTV LQLDPAVVKG ICLSAGSPVS HSALIARELG IGWICQQGEK LYAIQPEETL TLDVKTQRFN RQG
Length	473
Mol. Wt	51.58 kDa
pI	4.5 (calculated)
Extinction coefficient	27,390 - 28,390 (calc based on 11 Y, 2 W, and 8 C residues)
edit table

Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Modification Site	249	phosphorylation site at T249	probability greater than 75%	PMID:17938405
Domain	3..122	PF03610 PTS system fructose IIA component		PMID:19920124
Domain	387..467	PF00391 PEP-utilising enzyme, mobile domain		PMID:19920124
Domain	156..239	PF00381 PTS HPr component phosphorylation site		PMID:19920124
Domain	257..370	PF05524 PEP-utilising enzyme, N-terminal		PMID:19920124
edit table

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=dhaM taxon=562,83333 </beststructure>

Models

View models at:

edit table

</protect>

Structure figures

</protect>

Notes

Gene Product Resources

[back to top]

See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
edit table

Notes

Accessions in Other Databases

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:226524708	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945749	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004024	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P37349	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12399	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12399	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945749	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002261	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2299	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Expression

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

[back to top]

See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	19.418+/-0.135	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
Protein	E. coli K-12 MG1655	415	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	414	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	275	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
edit table

Notes

Transcription and Transcriptional Regulation

[back to top]

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
dhaKLM
edit table

Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

[back to top]

<protect><gbrowseImage> name=NC_000913:1248317..1248357 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]

<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
edit table

Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1198 (EcoliWiki Page)	NCBI GEO profiles for dhaM
microarray	GenExpDB:b1198 (EcoliWiki Page)	Summary of data for dhaM from multiple microarray studies
edit table

Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1248271..1248366) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ11; Well:E6^[5]
edit table

Notes

Accessions Related to dhaM Expression

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12399	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2299	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1198
EcoGene	EcoGene:EG12399	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002261	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004024	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

Evolution

[back to top]

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

[back to top]

See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	YCGC	From SHIGELLACYC
E. coli O157	YCGC	From ECOO157CYC
edit table

Do-It-Yourself Web Tools

Notes

Families

[back to top]

See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00391 PEP-utilising enzyme, mobile domain
Pfam (EcoliWiki Page)	PF03610 PTS system fructose IIA component
Superfamily (EcoliWiki Page)	SUPERFAMILY:47831
Superfamily (EcoliWiki Page)	SUPERFAMILY:52009
Superfamily (EcoliWiki Page)	SUPERFAMILY:53062
Superfamily (EcoliWiki Page)	SUPERFAMILY:55594
Pfam (EcoliWiki Page)	PF00381 PTS HPr component phosphorylation site
Pfam (EcoliWiki Page)	PF05524 PEP-utilising enzyme, N-terminal
EcoCyc	EcoCyc:EG12399	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12399	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002261	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2299	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0004024	Escherichia coli str. K-12 substr. MG1655
edit table

Notes

Links

[back to top]

Name	URL	Comments
edit table

References

[back to top]

See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

dhaM:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to dhaM Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

Categories

Navigation menu

Search