copA:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
copA |
|---|---|
| Mnemonic |
Copper |
| Synonyms |
ECK0478, b0484, JW0473, ybaR[1], ybaR |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
10.95 minutes |
MG1655: 510603..508099 |
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NC_012967: 483547..481043 |
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NC_012759: 410858..413362 |
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W3110 |
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W3110: 510603..508099 |
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DH10B: 449934..447430 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
508102 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
copAC113A |
C113A |
No effect |
seeded from UniProt:Q59385 | ||||
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copAC479A |
C479A |
Loss of copper resistance, transport and phosphoenzyme formation |
seeded from UniProt:Q59385 | ||||
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copAC481A,H |
C481A,H |
Loss of copper resistance, transport and phosphoenzyme formation |
seeded from UniProt:Q59385 | ||||
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copAC110A |
C110A |
No effect |
seeded from UniProt:Q59385 | ||||
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copA |
Deletion | ||||||
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copAC14A |
C14A |
No effect |
seeded from UniProt:Q59385 | ||||
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copAC17A |
C17A |
No effect |
seeded from UniProt:Q59385 | ||||
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ΔcopA (Keio:JW0473) |
deletion |
deletion |
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ΔcopA1::kan |
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ΔcopA767::kan |
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copA(del) |
Sensitivity to |
Sensitivity to antimicrobial agent Copper Ion. |
Experimental Strain: LMG194 |
See growth curve in figure 2. | |||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0473 |
Plasmid clone |
Status:Clone OK Primer 1:gccTCACAAACTATCGACCTGACCCTGGAC Primer 2:ccTTCCTTCGGTTTAAACCGCAGCAACCGG | |
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Kohara Phage |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 79% [8] | ||
|
Linked marker |
est. P1 cotransduction: 15% [8] | ||
|
copA::kan |
Strain |
CN2328 |
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pCN2345 |
Plasmid Clone |
copA with own promoter cloned into pBR322 |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Wasinger, VC & Humphery-Smith, I (1998) Small genes/gene-products in Escherichia coli K-12. FEMS Microbiol. Lett. 169 375-82 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ Rensing, C et al. (2000) CopA: An Escherichia coli Cu(I)-translocating P-type ATPase. Proc. Natl. Acad. Sci. U.S.A. 97 652-6 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ 9.0 9.1 Petersen, C & Møller, LB (2000) Control of copper homeostasis in Escherichia coli by a P-type ATPase, CopA, and a MerR-like transcriptional activator, CopR. Gene 261 289-98 PubMed
