asd:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

asd

Mnemonic

Aspartate semialdehyde deHase

Synonyms

ECK3419, b3433, JW3396, dap, hom, dap+hom[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

76.98 minutes 

MG1655: 3572901..3571798
<gbrowseImage> name=NC_000913:3571798..3572901 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3506332..3505229
<gbrowseImage> name=NC_012967:3505229..3506332 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3460292..3461395
<gbrowseImage> name=NC_012759:3460292..3461395 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4065537..4066640
<gbrowseImage> name=NC_007779:4065537..4066640 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3670646..3669543
<gbrowseImage> name=NC_010473:3669543..3670646 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3571798

Edman degradation

PMID:9298646[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

asdC135S

C135S

99.7% loss of activity

seeded from UniProt:P0A9Q9

asdC135A

C135A

Complete loss of activity

seeded from UniProt:P0A9Q9

asd-1

CGSC:5115


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3396

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCAAAAATGTTGGTTTTATCGG

Primer 2:CCgGCCAGTTGACGAAGCATCCG

10F5

Kohara Phage

Genobase

PMID:3038334[5]

16A5

Kohara Phage

Genobase

PMID:3038334[5]

glgP721::Tn10

Linked marker

CAG18638 = CGSC7451[6]

est. P1 cotransduction: 75% [7]
Synonyms:zhg-3086::Tn10, zhh-21::Tn10

zhg-50::Tn10

Linked marker

CAG18450 = CGSC7450[6]

est. P1 cotransduction: 50% [7]
Synonyms:zhf-50::Tn10, zhf-5::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10088

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10088

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000084

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947939

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0086

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011211

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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