alsK:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

alsK

Mnemonic

Allose

Synonyms

ECK4077, b4084, JW5724, yjcT[1], yjcT

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

92.78 minutes 

MG1655: 4305822..4304893
<gbrowseImage> name=NC_000913:4304893..4305822 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4286621..4285692
<gbrowseImage> name=NC_012967:4285692..4286621 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4243628..4244557
<gbrowseImage> name=NC_012759:4243628..4244557 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4312477..4311449
<gbrowseImage> name=NC_007779:4311449..4312477 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4406184..4405255
<gbrowseImage> name=NC_010473:4405255..4406184 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

alsKA73G

A73G

60-fold increase in catalytic efficiency for glucose phosphorylation. 45-fold increase in D-glucose affinity. No change in catalytic efficiency for D- allose phosphorylation

seeded from UniProt:P32718

alsKF145L

F145L

10-fold increase in catalytic efficiency for glucose phosphorylation. Slight increase in catalytic efficiency for D-allose phosphorylation

seeded from UniProt:P32718

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5724

Plasmid clone

Shigen

PMID:16769691[2]

Status:Clone OK

Primer 1:GCCCAAAAACAGCATAACGTCGT

Primer 2:CCAATAGGCTGATTCAAGGCATT

12A6

Kohara Phage

Genobase

PMID:3038334[3]

10H5

Kohara Phage

Genobase

PMID:3038334[3]

malF3089::Tn10

Linked marker

CAG12164 = CGSC7476[4]

est. P1 cotransduction: 3% [5]

zje-2241::Tn10

Linked marker

CAG18427 = CGSC7481[4]

est. P1 cotransduction: 98% [5]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11956

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11956

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001883

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948596

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1899

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013380

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  3. 3.0 3.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  4. 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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