alkA:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
alkA |
|---|---|
| Mnemonic |
Alkylation |
| Synonyms |
ECK2062, b2068, JW2053, aidA[1], aidA |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
46.23 minutes, 46.23 minutes |
MG1655: 2145564..2144716 |
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NC_012967: 2080726..2079878 |
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NC_012759: 2037199..2038047 |
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W3110 |
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W3110: 2149677..2148829 |
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DH10B: 2236572..2235724 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
2144716 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
alkAD238N |
D238N |
No catalytic activity, methylmethane sulfonate-sensitive |
seeded from UniProt:P04395 | ||||
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alkAD237N |
D237N |
More than 30% catalytic activity, methylmethane sulfonate-resistant |
seeded from UniProt:P04395 | ||||
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alkAQ124A |
Q124A |
Methylmethane sulfonate-resistant |
seeded from UniProt:P04395 | ||||
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alkAW218A |
W218A |
No catalytic activity, methylmethane sulfonate-sensitive |
seeded from UniProt:P04395 | ||||
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ΔalkA (Keio:JW2053) |
deletion |
deletion |
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ΔalkA766::kan |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2053 |
Plasmid clone |
Status:Clone OK Primer 1:GCCTATACCCTGAACTGGCAGCC Primer 2:CCTGCTTCGTCTGGTTGCCAGCC | |
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Kohara Phage |
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Kohara Phage |
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zef-3129::Tn10 |
Linked marker |
est. P1 cotransduction: 4% [7] | |
|
Linked marker |
est. P1 cotransduction: 29% [7] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Nakabeppu, Y et al. (1984) Structure and expression of the alkA gene of Escherichia coli involved in adaptive response to alkylating agents. J. Biol. Chem. 259 13730-6 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
