adk:Gene
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
<protect>
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
adk |
|---|---|
| Mnemonic |
Adenylate kinase |
| Synonyms |
ECK0468, b0474, JW0463, dnaW, plsA[1], plsA |
| edit table |
</protect>
Notes
dnaW and adk were shown to be allelic by Henson an Walker[2].
Location(s) and DNA Sequence
<protect>
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
10.7 minutes |
MG1655: 496399..497043 |
||
|
NC_012967: 469242..469886 |
||||
|
NC_012759: 399158..399802 |
||||
|
W3110 |
|
W3110: 496399..497043 |
||
|
DH10B: 435730..436374 |
||||
| edit table |
</protect>
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
496399 |
Edman degradation |
PMID:3027060[3] |
| |
| edit table |
<protect></protect>
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
adkP9G |
P9G |
No loss of enzyme activity |
seeded from UniProt:P69441 | ||||
|
adkG10V |
G10V |
No loss of enzyme activity |
seeded from UniProt:P69441 | ||||
|
adkK13Q |
K13Q |
Drastic reduction in enzyme activity |
seeded from UniProt:P69441 | ||||
|
adk-15(ts) |
temperature sensitive |
||||||
|
adk-2(ts) |
temperature sensitive |
||||||
|
adk-31(ts) |
temperature sensitive |
||||||
|
adk-3(ts) |
temperature sensitive |
||||||
|
adk-9(ts) |
temperature sensitive |
| |||||
| edit table |
<protect></protect>
Notes
Genetic Interactions
<protect>
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
</protect>
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0463 |
Plasmid clone |
Status:Clone OK Primer 1:GCCCGTATCATTCTGCTTGGCGC Primer 2:CCGCCGAGGATTTTTTCCAGATC | |
|
Kohara Phage |
|||
|
Kohara Phage |
|||
|
Linked marker |
est. P1 cotransduction: 22% [10] | ||
|
Linked marker |
est. P1 cotransduction: 86% [10] | ||
| edit table |
<protect></protect>
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
<protect></protect>
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Henson, JM et al. (1982) The Escherichia coli dnaW mutation is an allele of the adk gene. Mol. Gen. Genet. 186 488-92 PubMed
- ↑ Saint Girons, I et al. (1987) Structural and catalytic characteristics of Escherichia coli adenylate kinase. J. Biol. Chem. 262 622-9 PubMed
- ↑ Lu, Q & Inouye, M (1996) Adenylate kinase complements nucleoside diphosphate kinase deficiency in nucleotide metabolism. Proc. Natl. Acad. Sci. U.S.A. 93 5720-5 PubMed
- ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
- ↑ Wilkins, MR et al. (1998) Protein identification with N and C-terminal sequence tags in proteome projects. J. Mol. Biol. 278 599-608 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 9.0 9.1 CGSC: The Coli Genetics Stock Center
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
