aaeB:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

aaeB

Mnemonic

Aromatic (carboxylic) acid efflux

Synonyms

ECK3229, b3240, JW3209, yhcP[1], yhcP

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

72.94 minutes 

MG1655: 3386210..3384243
<gbrowseImage> name=NC_000913:3384243..3386210 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3314118..3316085
<gbrowseImage> name=NC_012967:3314118..3316085 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3271391..3273358
<gbrowseImage> name=NC_012759:3271391..3273358 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3388043..3386076
<gbrowseImage> name=NC_007779:3386076..3388043 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Strain DH10B

T50P

Nonsynonomous mutation

PMID:18245285[2]

ASAP

This is a SNP in E. coli K-12 Strain DH10B

ΔaaeB (Keio:JW3209)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10433[4]

ΔaaeB765::kan

PMID:16738554[3]

CGSC:99868


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3209

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCGGTATTTTCTCCATTGCTAA

Primer 2:CCACTATCGGTCAACGCATGTTG

1E3

Kohara Phage

Genobase

PMID:3038334[6]

3G9

Kohara Phage

Genobase

PMID:3038334[6]

zhc-6::Tn10

Linked marker

CAG12153 = CGSC7442[4]

est. P1 cotransduction: 47% [7]
Synonyms:zha-6::Tn10

acrF3083::Tn10

Linked marker

CAG12075 = CGSC7447[4]

est. P1 cotransduction: 30% [7]
Synonyms:zhd-3083::Tn10, zhe-3083::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7685

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12822

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004232

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947747

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2673

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010629

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Durfee, T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J. Bacteriol. 190 2597-606 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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