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On One Page

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Expression|

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Standard Name	10
Gene Synonym(s)	Phage_T7_10A, Phage_T7_10B
Product Desc.	major capsid protein
Product Synonyms(s)	Phage_T7_10A, Phage_T7_10B
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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<protect> See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	10
Mnemonic
Synonyms	Phage_T7_10A, Phage_T7_10B
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Notes

Location(s) and DNA Sequence

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<protect> <protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
		NC_1604: 22868..24105 <gbrowseImage> name=NC_001604:22868..24105 source=bacteriophage_T7 preset=GeneLocation </gbrowseImage>	GBrowse (Bacteriophage T7)	FASTA Dump
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Notes

Sequence Features

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<protect> See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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<protect> See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
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Notes

Mutations that abolish the frameshifting to make the minor 10B protein are viable^[1]

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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<protect> See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

<protect> See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	10A, 10B
Synonyms	Phage_T7_10A, Phage_T7_10B
Product description	major capsid protein
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology <protect> See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
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Interactions <protect> See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

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Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties <protect> See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	MASMTGGQQM GTNQGKGVVA AGDKLALFLK VFGGEVLTAF ARTSVTTSRH MVRSISSGKS AQFPVLGRTQ AAYLAPGENL DDKRKDIKHT EKVITIDGLL TADVLIYDIE DAMNHYDVRS EYTSQLGESL AMAADGAVLA EIAGLCNVES KYNENIEGLG TATVIETTQN KAALTDQVAL GKEIIAALTK ARAALTKNYV PAADRVFYCD PDSYSAILAA LMPNAANYAA LIDPEKGSIR NVMGFEVVEV PHLTAGGAGT AREGTTGQKH VFPANKGEGN VKVAKDNVIG LFMHRSAVGT VKLRDLALER ARRANFQADQ IIAKYAMGHG GLRPEAAGAV VFKVE	MASMTGGQQM GTNQGKGVVA AGDKLALFLK VFGGEVLTAF ARTSVTTSRH MVRSISSGKS AQFPVLGRTQ AAYLAPGENL DDKRKDIKHT EKVITIDGLL TADVLIYDIE DAMNHYDVRS EYTSQLGESL AMAADGAVLA EIAGLCNVES KYNENIEGLG TATVIETTQN KAALTDQVAL GKEIIAALTK ARAALTKNYV PAADRVFYCD PDSYSAILAA LMPNAANYAA LIDPEKGSIR NVMGFEVVEV PHLTAGGAGT AREGTTGQKH VFPANKGEGN VKVAKDNVIG LFMHRSAVGT VKLRDLALER ARRANFQADQ IIAKYAMGHG GLRPEAAGAV VFQSGVMLGV ASTVAASPEE ASVTSTEETL TPAQEAARTR AANKARKEAE LAAATAEQ
Length	345	398
Mol. Wt	36.545 kDa	41.83 kDa
pI	7.2 (calculated)	6.4 (calculated)
Extinction coefficient	14,900 - 15,150 (calc based on 10 Y, 0 W, and 2 C residues)	14,900 - 15,150 (calc based on 10 Y, 0 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
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<motif_map/>

Structure
<protect> See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=Phage_T7_10A taxon=10760 </beststructure>

Models

View models at:

PSI-PRED
Genomes TO Protein (GTOP)

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Structure figures

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Notes

Gene 10 makes two protein products via a programmed -1 frameshift near the end of the shorter 10A product^[2]^[1]

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
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Notes

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_001604:22948..22988 source=bacteriophage_T7 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

Turnover and Regulation of Turnover

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Notes

Experimental

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<protect> Mutations Affecting Expression <protect> See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies <protect> See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
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Expression Resources <protect> See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to Phage T7 10A Expression

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<protect> See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
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Do-It-Yourself Web Tools

Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database	Accession	Notes
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 Condron, BG et al. (1991) Frameshifting in gene 10 of bacteriophage T7. J. Bacteriol. 173 6998-7003 PubMed
↑ Sipley, J et al. (1991) Analysis of bacteriophage T7 gene 10A and frameshifted 10B proteins. Gene Expr. 1 127-36 PubMed

Phage T7 10:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to Phage T7 10A Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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