proP:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

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Standard name

ProP

Synonyms

proline/glycine betaine transporter[1], B4111[2][1], ProP[2][1]

Product description

ProP proline/betaine MFS transporter[2][3]

Proline/betaine permease, minor; osmosensor/osmoregulator[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference(s) Evidence Code with/from Aspect Notes Status
GO:0006810

transport

PMID:6355059[5]

IGI: Inferred from Genetic Interaction

P

Seeded from EcoCyc 12.5 [6]

complete

GO:0015292

uniporter activity

PMID:15205426[7]

IGI: Inferred from Genetic Interaction

F

Seeded from EcoCyc [8]

complete

GO:0015295

solute:hydrogen symporter activity

PMID:3049595[9]

IGI: Inferred from Genetic Interaction

F

Seeded from EcoCyc [8]

complete

GO:0042538

hyperosmotic salinity response

PMID:3082857[10]

IGI: Inferred from Genetic Interaction

P

Seeded from EcoCyc [8]

complete

GO:0016021

integral to membrane

PMID:10026245[11]

IDA: Inferred from Direct Assay

C

complete

GO:0016020

membrane

GO_REF:0000002
GO_REF:0000004
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR005829
SP_KW:KW-0397
SP_KW:KW-0472

C

Seeded from EcoCyc 12.5 [6]

complete

GO:0006810

transport

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR004736
InterPro:IPR005828
InterPro:IPR005829
InterPro:IPR007114
SP_KW:KW-0813

P

Seeded from EcoCyc 12.5 [6]

complete

GO:0006561

proline biosynthetic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0005886

plasma membrane

GO_REF:0000004
GO_REF:0000004
GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997
SP_KW:KW-1003
SP_SL:SL-0037

C

Seeded from EcoCyc [8]

complete

GO:0015293

symporter activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0769

F

Seeded from EcoCyc [8]

complete

GO:0016021

integral to membrane

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR004736
InterPro:IPR005828
InterPro:IPR007114
SP_KW:KW-0812

C

Seeded from EcoCyc 12.5 [6]

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence
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Notes

Proline transporter activity is sensitive to the osmolality of the growth medium. Activation and polar localization depend on cardiolipin content of the membrane.

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Source Notes

plasma membrane

C-terminus localized in the cytoplasm with 12 predicted transmembrane domains

Daley et al. (2005) [12]

plasma membrane

From EcoCyc[3]

Pole

Fluorescence microscopy PMID:17504273[13] PMID:18326496[14]


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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MLKRKKVKPI TLRDVTIIDD GKLRKAITAA SLGNAMEWFD FGVYGFVAYA LGKVFFPGAD
PSVQMVAALA TFSVPFLIRP LGGLFFGMLG DKYGRQKILA ITIVIMSIST FCIGLIPSYD
TIGIWAPILL LICKMAQGFS VGGEYTGASI FVAEYSPDRK RGFMGSWLDF GSIAGFVLGA
GVVVLISTIV GEANFLDWGW RIPFFIALPL GIIGLYLRHA LEETPAFQQH VDKLEQGDRE
GLQDGPKVSF KEIATKYWRS LLTCIGLVIA TNVTYYMLLT YMPSYLSHNL HYSEDHGVLI
IIAIMIGMLF VQPVMGLLSD RFGRRPFVLL GSVALFVLAI PAFILINSNV IGLIFAGLLM
LAVILNCFTG VMASTLPAMF PTHIRYSALA AAFNISVLVA GLTPTLAAWL VESSQNLMMP
AYYLMVVAVV GLITGVTMKE TANRPLKGAT PAASDIQEAK EILVEHYDNI EQKIDDIDHE
IADLQAKRTR LVQQHPRIDE
Length

500

Mol. Wt

54.848 kDa

pI

7.2 (calculated)

Extinction coefficient

63,830 - 64,330 (calc based on 17 Y, 7 W, and 4 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References
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<motif_map/>

tmhmm.php?gene=proP&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=proP taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:PROP-MONOMER

UniProt (EcoliWiki Page)

UniProt:P0C0L7

PFAM (EcoliWiki Page)

PFAM:PF00083

RefSeq (EcoliWiki Page)

RefSeq:NP_418535


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Stalmach, ME et al. (1983) Two proline porters in Escherichia coli K-12. J. Bacteriol. 156 481-6 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  7. ↑ Buurman, ET et al. (2004) Multiple paths for nonphysiological transport of K+ in Escherichia coli. J Bacteriol 186 4238-45 PubMed
  8. ↑ 8.0 8.1 8.2 8.3 8.4 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  9. ↑ Milner, JL et al. (1988) Proline porter II is activated by a hyperosmotic shift in both whole cells and membrane vesicles of Escherichia coli K12. J Biol Chem 263 14900-5 PubMed
  10. ↑ Gowrishankar, J (1986) proP-mediated proline transport also plays a role in Escherichia coli osmoregulation. J Bacteriol 166 331-3 PubMed
  11. ↑ Racher, KI et al. (1999) Purification and reconstitution of an osmosensor: transporter ProP of Escherichia coli senses and responds to osmotic shifts. Biochemistry 38 1676-84 PubMed
  12. ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
  13. ↑ Romantsov, T et al. (2007) Cardiolipin promotes polar localization of osmosensory transporter ProP in Escherichia coli. Mol. Microbiol. 64 1455-65 PubMed
  14. ↑ Romantsov, T et al. (2008) Cardiolipin controls the osmotic stress response and the subcellular location of transporter ProP in Escherichia coli. J. Biol. Chem. 283 12314-23 PubMed

Categories

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