alaS:Expression

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Overview Transcription Translation Turnover Experimental Links References Suggestions

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

1.83E+03

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Growth Rate(min):
  • other:

emPAI

PMID:18304323[1]

Protein

Ecoli K-12

86.147+/-0.477

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Growth Rate(min): *
  • other: *

Single Molecule Fluorescence

PMID:20671182[2]

mRNA

Ecoli K-12

0.243536122

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Growth Rate(min): *
  • other: *

by RNA_Seq

PMID:20671182[2]

Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

alaS

Figure courtesy of RegulonDB


Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

alaS regulates it's own transcription [3]

Translation and Regulation of Translation

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<gbrowseImage> name=NC_000913:2820013..2820053 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.


Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2697 (EcoliWiki Page)

NCBI GEO profiles for alaS

microarray

GenExpDB:b2697 (EcoliWiki Page)

Summary of data for alaS from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (2819936..2820163) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ10; Well:B11[4]

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Notes

Accessions Related to alaS Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10034

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0033

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2697

EcoGene

EcoGene:EG10034

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000031

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008869

Escherichia coli str. K-12 substr. MG1655


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Ishihama, Y et al. (2008) Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics 9 102 PubMed
  2. ↑ 2.0 2.1 Taniguchi, Y et al. (2010) Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells. Science 329 533-8 PubMed
  3. ↑ Putney, SD & Schimmel, P (1981) An aminoacyl tRNA synthetase binds to a specific DNA sequence and regulates its gene transcription. Nature 291 632-5 PubMed
  4. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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