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!align=left {{table_heading_style}} |Standard name
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''cfa''  
''cfa''  
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!align=left {{table_heading_style}} |Mnemonic
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!align=left {{table_heading_style}} |Synonyms
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''ECK1657, b1661, JW1653, cdfA''<ref name='LIB:Riley_2006'/>
''ECK1657, b1661, JW1653, cdfA''<ref name='LIB:Riley_2006'/>


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Synonyms:zdh-925::Tn10  
Synonyms:zdh-925::Tn10  


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Revision as of 00:19, 9 January 2010

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

cfa

Mnemonic
Synonyms

ECK1657, b1661, JW1653, cdfA[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

37.49 minutes 

MG1655: 1739437..1740585
gbrowse_img.php?coord=1739437..1740585&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 1718680..1719828
gbrowse_img.php?coord=1718680..1719828&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 1743127..1744275
gbrowse_img.php?coord=1743127..1744275&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 1830008..1831156
gbrowse_img.php?coord=1830008..1831156&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δcfa (Keio:JW1653)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9407[3]

cfa

PMID:12519969[4]

ASAP

At position 635 in Minus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20416

cfa

PMID:12519969[4]

ASAP

At position 635 in Minus orientation, contains plasmid pKD46.
ASAP strain name: FB20417

cfa-1

CGSC:10811

cfa-2

PMID:6092874[5] PMID:3519583[6]

CGSC:17027

cfa-3

PMID:3519583[6] PMID:6092874[5]

CGSC:17030

cfa-4

PMID:3519583[6] PMID:6092874[5]

CGSC:17032

cfa-5::kan

PMID:10411742[7] PMID:3038679[8]

CGSC:64828

cfa-0

CGSC:73935

Δcfa-739::kan

PMID:16738554[2]

CGSC:100276


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1653

Plasmid clone

Shigen

PMID:16769691[9]

Status:Clone OK

Primer 1:GCCAGTTCATCGTGTATAGAAGA

Primer 2:CCGCGAGCCACTCGAAGGCCGTT

zdd-235::Tn10

Linked marker

CAG18461 = CGSC7378[3]

est. P1 cotransduction: % [10]
Synonyms:zdc-235::Tn10

zdi-925::Tn10

Linked marker

CAG12151 = CGSC7385[3]

est. P1 cotransduction: 21% [10]
Synonyms:zdh-925::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11531

EchoBASE (EcoliWiki Page)

EchoBASE:EB1493

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1661

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2055

EcoGene (EcoliWiki Page)

EcoGene:EG11531

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:CFA

CGSC (EcoliWiki Page)

CGSC:10810

ASAP (EcoliWikiPage)

ASAP:ABE-0005555


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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 Glasner, JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res. 31 147-51 PubMed
  5. ↑ 5.0 5.1 5.2 Grogan, DW & Cronan, JE Jr (1984) Genetic characterization of the Escherichia coli cyclopropane fatty acid (cfa) locus and neighboring loci. Mol. Gen. Genet. 196 367-72 PubMed
  6. ↑ 6.0 6.1 6.2 Grogan, DW & Cronan, JE Jr (1986) Characterization of Escherichia coli mutants completely defective in synthesis of cyclopropane fatty acids. J. Bacteriol. 166 872-7 PubMed
  7. ↑ Chang, YY & Cronan, JE Jr (1999) Membrane cyclopropane fatty acid content is a major factor in acid resistance of Escherichia coli. Mol. Microbiol. 33 249-59 PubMed
  8. ↑ Fellay, R et al. (1987) Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of gram-negative bacteria. Gene 52 147-54 PubMed
  9. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  10. ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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