arcB:Gene: Difference between revisions

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[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:arc genes]]
[[Category:arc genes]][[Category:Gene_List:DH10B]]

Revision as of 16:15, 6 April 2009

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

arcB

Mnemonic
Synonyms

ECK3200, b3210, JW5536[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

72.18 minutes 

MG1655: 3351047..3348711
gbrowse_img.php?coord=3347711..3352047&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 3352880..3350544
gbrowse_img.php?coord=3349544..3353880&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔarcB (Keio:JW5536)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC11593[3]

arcB41(del-ins,::FRT)

PMID:10829079[4]

CGSC:65938

arcB40(del-ins)::cat

PMID:10829079[4]

CGSC:65940

ΔarcB738::kan

PMID:16738554[2]

CGSC:112242


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5536

Plasmid clone

Shigen

PMID:16769691[5]

Status:cloned as old JW

Primer 1:GCCAAGCAAATTCGTCTGCTGGC

Primer 2:CCTTTTTTAGTGGCTTTTGCCAC

sfsB203::Tn10

Linked marker

CAG12072 = CGSC7440[3]

est. P1 cotransduction: 54% [6]
Synonyms:zgj-203::Tn10, zha-203::Tn10

zhc-6::Tn10

Linked marker

CAG12153 = CGSC7442[3]

est. P1 cotransduction: 59% [6]
Synonyms:zha-6::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10062

EchoBASE (EcoliWiki Page)

EchoBASE:EB0060

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3210

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3970

EcoGene (EcoliWiki Page)

EcoGene:EG10062

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:ARCB

CGSC (EcoliWiki Page)

CGSC:29063

ASAP (EcoliWikiPage)

ASAP:ABE-0010537


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Notes

Links

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Name URL Comments
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References

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  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 Datsenko, KA & Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97 6640-5 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).


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