eno:Gene Product(s): Difference between revisions
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<big>'''Gene Ontology'''</big><br /> | <big>'''Gene Ontology'''</big><br /><!--box uid=421aa90e079fa326b6494f812ad13e79.25894.C46aabd48d3832--> | ||
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| | | | ||
GO:0046872 | |||
| | | | ||
metal ion binding | metal ion binding | ||
| | | | ||
GO_REF:0000004 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
SP_KW:KW-0479 | |||
| | | | ||
F | F | ||
| Line 102: | Line 102: | ||
| | | | ||
GO:0016829 | |||
| | | | ||
lyase activity | lyase activity | ||
| | | | ||
GO_REF:0000004 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
SP_KW:KW-0456 | |||
| | | | ||
F | F | ||
| Line 121: | Line 121: | ||
| | | | ||
GO:0006096 | |||
| | | | ||
glycolysis | glycolysis | ||
| | | | ||
GO_REF:0000002<br />GO_REF:0000004<br />GO_REF:0000020 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
InterPro:IPR000941<br />SP_KW:KW-0324<br />HAMAP:MF_00318 | |||
| | | | ||
P | P | ||
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| | | | ||
GO:0005856 | |||
| | | | ||
cytoskeleton | cytoskeleton | ||
| | | | ||
GO_REF:0000004 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
SP_KW:KW-0206 | |||
| | | | ||
C | C | ||
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| | | | ||
GO:0005737 | |||
| | | | ||
cytoplasm | cytoplasm | ||
| | | | ||
GO_REF:0000004 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
SP_KW:KW-0963 | |||
| | | | ||
C | C | ||
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| | | | ||
GO:0004634 | |||
| | | | ||
phosphopyruvate hydratase activity | phosphopyruvate hydratase activity | ||
| | | | ||
GO_REF:0000002<br />GO_REF:0000003<br />GO_REF:0000020 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
InterPro:IPR000941<br />EC:4.2.1.11<br />HAMAP:MF_00318 | |||
| | | | ||
F | F | ||
| Line 197: | Line 197: | ||
| | | | ||
GO:0000287 | |||
| | | | ||
magnesium ion binding | magnesium ion binding | ||
| | | | ||
GO_REF:0000004<br />GO_REF:0000020 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
SP_KW:KW-0460<br />HAMAP:MF_00318 | |||
| | | | ||
F | F | ||
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| | | | ||
GO:0000015 | |||
| | | | ||
phosphopyruvate hydratase complex | phosphopyruvate hydratase complex | ||
| | | | ||
GO_REF:0000002 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| | | | ||
InterPro:IPR000941 | |||
| | | | ||
C | C | ||
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| | | | ||
GO:0004634 | |||
| | | | ||
phosphopyruvate hydratase activity | phosphopyruvate hydratase activity | ||
| | | | ||
PMID:4942326 | |||
| | | | ||
IDA: Inferred from Direct Assay | IDA: Inferred from Direct Assay | ||
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| | | | ||
GO:0005515 | |||
| | | | ||
protein binding | protein binding | ||
| | | | ||
PMID:15236960 | |||
| | | | ||
IPI: Inferred from Physical Interaction | IPI: Inferred from Physical Interaction | ||
| | | | ||
UniProtKB:P21513 | |||
| | | | ||
F | F | ||
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| | | | ||
GO:0005624 | |||
| | | | ||
membrane fraction | membrane fraction | ||
| | | | ||
PMID:16858726 | |||
| | | | ||
IDA: Inferred from Direct Assay | IDA: Inferred from Direct Assay | ||
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| | | | ||
GO:0042802 | |||
| | | | ||
identical protein binding | identical protein binding | ||
| | | | ||
PMID:15236960 | |||
| | | | ||
IPI: Inferred from Physical Interaction | IPI: Inferred from Physical Interaction | ||
| | | | ||
UniProtKB:P0A6P9 | |||
| | | | ||
F | F | ||
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| | | | ||
GO:0009061 | |||
| | | | ||
anaerobic respiration | anaerobic respiration | ||
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| | | | ||
GO:0006094 | |||
| | | | ||
gluconeogenesis | gluconeogenesis | ||
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| | | | ||
GO:0004634 | |||
| | | | ||
phosphopyruvate hydratase activity | phosphopyruvate hydratase activity | ||
| | | | ||
PMID:4942326 | |||
| | | | ||
IDA: Inferred from Direct Assay | IDA: Inferred from Direct Assay | ||
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Protein | Protein | ||
| | | | ||
Subunits of [[:Category:Complex:enolase|enolase]] | |||
| | | | ||
could be indirect | could be indirect | ||
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Protein | Protein | ||
| | | | ||
aroF<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:16606699 | ||
| | |||
Experiment(s):EBI-1143906<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
rplI<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:16606699 | ||
| | |||
Experiment(s):EBI-1143906<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
yfeX<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:16606699 | ||
| | |||
Experiment(s):EBI-1143906<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
rplW<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:16606699 | ||
| | |||
Experiment(s):EBI-1143906<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
nadE<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:16606699 | ||
| | |||
Experiment(s):EBI-1143906<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
rdgC<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:16606699 | ||
| | |||
Experiment(s):EBI-1143906<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
ybdN<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:16606699 | ||
| | |||
Experiment(s):EBI-1143906<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
yqjK<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:16606699 | ||
| | |||
Experiment(s):EBI-1143906<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
racR<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:16606699 | ||
| | |||
Experiment(s):EBI-1143906<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
nanE<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:16606699 | ||
| | |||
Experiment(s):EBI-1143906<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
dnaK<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:15690043 | ||
| | |||
Experiment(s):EBI-880413, EBI-885840<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
fabZ<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:15690043 | ||
| | |||
Experiment(s):EBI-880413<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
gltX<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:15690043 | ||
| | |||
Experiment(s):EBI-880413<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
pnp<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:15690043 | ||
| | |||
Experiment(s):EBI-880413, EBI-883913, EBI-891089, EBI-885840<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
yniC<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:15690043 | ||
| | |||
Experiment(s):EBI-880413<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
pinR<br /> | |||
| | | | ||
| | | | ||
Experiment(s): | PMID:15690043 | ||
| | |||
Experiment(s):EBI-885840<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
rpsE<br /> | |||
| | | | ||
| | | | ||
PMID:15690043 | |||
| | | | ||
Experiment(s):EBI-885840<br /> | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
[[ | Subunits of [[:Category:Complex:degradosome|degradosome]] | ||
| | |||
could be indirect | |||
| | | | ||
| | | | ||
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===Notes=== | ===Notes=== | ||
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<span id="Domains/Motifs/Modification Sites"><big>'''Domains/Motifs/Modification Sites'''</big></span> | <span id="Domains/Motifs/Modification Sites"><big>'''Domains/Motifs/Modification Sites'''</big></span> | ||
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<span id="Structure"></span><big>'''Structure'''</big> | <span id="Structure"></span><big>'''Structure'''</big> | ||
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==References== | ==References== | ||
{{BacktoTop}} | {{BacktoTop}} | ||
<references/> | <references/> | ||
__NOTOC__ | __NOTOC__ | ||
Revision as of 22:24, 16 February 2009
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
Eno |
|---|---|
| Synonyms | |
| Product description |
Component of enolase[2][3]; degradosome[3] |
| EC number (for enzymes) | |
| edit table |
Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference(s) | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0046872 |
metal ion binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0016829 |
lyase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0006096 |
glycolysis |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0005856 |
cytoskeleton |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0004634 |
phosphopyruvate hydratase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0000287 |
magnesium ion binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0000015 |
phosphopyruvate hydratase complex |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0004634 |
phosphopyruvate hydratase activity |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0005515 |
protein binding |
IPI: Inferred from Physical Interaction |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0005624 |
membrane fraction |
IDA: Inferred from Direct Assay |
C |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0042802 |
identical protein binding |
IPI: Inferred from Physical Interaction |
F |
Seeded from EcoCyc 12.5 [4] |
complete | |||
| GO:0009061 |
anaerobic respiration |
P |
Seeded from EcoCyc 11.1[3]. |
required fields missing | ||||
| GO:0006094 |
gluconeogenesis |
P |
Seeded from EcoCyc 11.1[3]. |
required fields missing | ||||
| GO:0004634 |
phosphopyruvate hydratase activity |
IDA: Inferred from Direct Assay |
F |
purification, assay according to ref 5, which is not in Pubmed |
complete | |||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of enolase |
could be indirect |
||
|
Protein |
aroF |
Experiment(s):EBI-1143906 | ||
|
Protein |
rplI |
Experiment(s):EBI-1143906 | ||
|
Protein |
yfeX |
Experiment(s):EBI-1143906 | ||
|
Protein |
rplW |
Experiment(s):EBI-1143906 | ||
|
Protein |
nadE |
Experiment(s):EBI-1143906 | ||
|
Protein |
rdgC |
Experiment(s):EBI-1143906 | ||
|
Protein |
ybdN |
Experiment(s):EBI-1143906 | ||
|
Protein |
yqjK |
Experiment(s):EBI-1143906 | ||
|
Protein |
racR |
Experiment(s):EBI-1143906 | ||
|
Protein |
nanE |
Experiment(s):EBI-1143906 | ||
|
Protein |
dnaK |
Experiment(s):EBI-880413, EBI-885840 | ||
|
Protein |
fabZ |
Experiment(s):EBI-880413 | ||
|
Protein |
gltX |
Experiment(s):EBI-880413 | ||
|
Protein |
pnp |
Experiment(s):EBI-880413, EBI-883913, EBI-891089, EBI-885840 | ||
|
Protein |
yniC |
Experiment(s):EBI-880413 | ||
|
Protein |
pinR |
Experiment(s):EBI-885840 | ||
|
Protein |
rpsE |
Experiment(s):EBI-885840 | ||
|
Protein |
Subunits of degradosome |
could be indirect |
| |
| edit table |
Notes
IMP for enolase activity probably in [10] (based on abstract. need to get paper and read).
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Notes |
|---|---|---|---|
|
cytoplasm |
From EcoCyc[3] | ||
| edit table |
Notes
Structure and Physical Properties
Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Sequence |
MSKIVKIIGR EIIDSRGNPT VEAEVHLEGG FVGMAAAPSG ASTGSREALE LRDGDKSRFL GKGVTKAVAA VNGPIAQALI GKDAKDQAGI DKIMIDLDGT ENKSKFGANA ILAVSLANAK AAAAAKGMPL YEHIAELNGT PGKYSMPVPM MNIINGGEHA DNNVDIQEFM IQPVGAKTVK EAIRMGSEVF HHLAKVLKAK GMNTAVGDEG GYAPNLGSNA EALAVIAEAV KAAGYELGKD ITLAMDCAAS EFYKDGKYVL AGEGNKAFTS EEFTHFLEEL TKQYPIVSIE DGLDESDWDG FAYQTKVLGD KIQLVGDDLF VTNTKILKEG IEKGIANSIL IKFNQIGSLT ETLAAIKMAK DAGYTAVISH RSGETEDATI ADLAVGTAAG QIKTGSMSRS DRVAKYNQLI RIEEALGEKA PYNGRKEIKG QA |
|---|---|
| Mol. Wt |
45.65 kDa (calc) [2] |
| pI |
5.64[2] |
| Extinction coefficient |
21890 - 22015 (calc based on 11 Y, 1 W, and 1 C residues) |
| edit table |
Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
| Type | Residues | Description | Notes |
|---|---|---|---|
| edit table |
Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.
| Structures |
<beststructure>eno 562</beststructure> |
|---|---|
| Models |
View models at: |
| edit table |
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
- ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 3.4 3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.00 4.01 4.02 4.03 4.04 4.05 4.06 4.07 4.08 4.09 4.10 4.11 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 5.0 5.1 Spring, TG & Wold, F (1971) The purification and characterization of Escherichia coli enolase. J. Biol. Chem. 246 6797-802 PubMed
- ↑ 6.0 6.1 Callaghan, AJ et al. (2004) Studies of the RNA degradosome-organizing domain of the Escherichia coli ribonuclease RNase E. J. Mol. Biol. 340 965-79 PubMed
- ↑ Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
- ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
- ↑ 9.0 9.1 9.2 9.3 9.4 9.5 9.6 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
- ↑ Irani, MH & Maitra, PK (1976) Glyceraldehyde 3-p dehydrogenase, glycerate 3-P kinase and enolase mutants of Escherichia coli: genetic studies. Mol. Gen. Genet. 145 65-71 PubMed
Categories
- GO:0046872 ! metal ion binding
- GO:0016829 ! lyase activity
- GO:0006096 ! glycolytic process
- GO:0005856 ! cytoskeleton
- GO:0005737 ! cytoplasm
- GO:0004634 ! phosphopyruvate hydratase activity
- GO:0000287 ! magnesium ion binding
- GO:0000015 ! phosphopyruvate hydratase complex
- GO:0005515 ! protein binding
- GO:0005624 ! obsolete membrane fraction
- GO:0042802 ! identical protein binding
- GO:0009061 ! anaerobic respiration
- GO:0006094 ! gluconeogenesis
- Proteins
- GO:0006096 ! glycolysis
- Complex:enolase
- Complex:degradosome
- Location:cytoplasm
- Pathway:Glycolysis
- GO Annotation by EcoliWiki Team