aat:Gene: Difference between revisions
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!{{table_heading_style}} |Standard name | !align=left {{table_heading_style}} |Standard name | ||
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''aat'' | ''aat'' | ||
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!{{table_heading_style}} |Mnemonic | !align=left {{table_heading_style}} |Mnemonic | ||
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!{{table_heading_style}} |Synonyms | !align=left {{table_heading_style}} |Synonyms | ||
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''ECK0876, b0885, JW0868, ycaA, G8002''<ref name='LIB:Riley_2006'/><ref name='LIB:EcoCyc10.6'/> | ''ECK0876, b0885, JW0868, ycaA, G8002''<ref name='LIB:Riley_2006'/><ref name='LIB:EcoCyc10.6'/> | ||
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===Notes=== | ===Notes=== | ||
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!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | !|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | ||
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MG1655 | MG1655 | ||
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{{GBrowseFastaLink|ecoli|NC_000913|925951|926655|FASTA Dump}} | {{GBrowseFastaLink|ecoli|NC_000913|925951|926655|FASTA Dump}} | ||
*[http://biocyc.org/ECOLI/sequence?object=EG11112 EcoCyc Sequence Dump] | *[http://biocyc.org/ECOLI/sequence?object=EG11112 EcoCyc Sequence Dump] | ||
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W3110 | W3110 | ||
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{{GBrowseFastaLink|ecoli_W3110|AC_000091|927150|927854|FASTA Dump}} | {{GBrowseFastaLink|ecoli_W3110|AC_000091|927150|927854|FASTA Dump}} | ||
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===Notes=== | ===Notes=== | ||
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!Feature type!!Location!!Description | !|Feature type!!Location!!Description | ||
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| | |[[PMID:16738554]]<ref name=PMID:16738554/> | ||
[[PMID:16738554]]<ref name=PMID:16738554/> | |||
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=107180 CGSC8899]<ref name='LIB:cgsc'/> | [http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=107180 CGSC8899]<ref name='LIB:cgsc'/> | ||
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MS845 has only 50% the tryptophanase activity of parent strain. | MS845 has only 50% the tryptophanase activity of parent strain. | ||
| | |[[PMID:137233]]<ref name=PMID:137233/> | ||
[[PMID:137233]]<ref name=PMID:137233/> | |||
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[[cgscmutation:8542|CGSC:8542]] | [[cgscmutation:8542|CGSC:8542]] | ||
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| | |[[PMID:16738554]]<ref name=PMID:16738554/> | ||
[[PMID:16738554]]<ref name=PMID:16738554/> | |||
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[[cgscmutation:99874|CGSC:99874]] | [[cgscmutation:99874|CGSC:99874]] | ||
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Cells appear as clubs,spheres, or ellipsoids. Normal rod-shaped morphology was restored by addition of D-alanine or D-glutamic | Cells appear as clubs,spheres, or ellipsoids. Normal rod-shaped morphology was restored by addition of D-alanine or D-glutamic | ||
acid to the medium. | acid to the medium. | ||
| | |[[PMID:137233]]<ref name=PMID:137233/> | ||
[[PMID:137233]]<ref name=PMID:137233/> | |||
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[[cgscmutation:8542|CGSC:8542]] | [[cgscmutation:8542|CGSC:8542]] | ||
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When aspartate was the sole nitrogen source, the 'aat-1' mutant strain (MS845 F'128) grew about twice as fast as its parent (doubling times of 2.3 and 4.8 h, respectively). | When aspartate was the sole nitrogen source, the 'aat-1' mutant strain (MS845 F'128) grew about twice as fast as its parent (doubling times of 2.3 and 4.8 h, respectively). | ||
| | |[[PMID:137233]]<ref name=PMID:137233/> | ||
[[PMID:137233]]<ref name=PMID:137233/> | |||
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[[cgscmutation:8542|CGSC:8542]] | [[cgscmutation:8542|CGSC:8542]] | ||
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===Notes=== | ===Notes=== | ||
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!Resource!!Resource Type!!Source!!Notes/Reference | !|Resource!!Resource Type!!Source!!Notes/Reference | ||
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JW0868 | JW0868 | ||
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen] | [http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen] | ||
| | |[[PMID:16769691]]<ref name=PMID:16769691/> | ||
[[PMID:16769691]]<ref name=PMID:16769691/> | |||
Status:Clone OK | Status:Clone OK | ||
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Primer 2:CCTTCTTGTGGTGAAAACAAGCA | Primer 2:CCTTCTTGTGGTGAAAACAAGCA | ||
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zbh-29::Tn10 | zbh-29::Tn10 | ||
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CAG18493 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39400 CGSC7353]<ref name='LIB:cgsc'/> | CAG18493 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39400 CGSC7353]<ref name='LIB:cgsc'/> | ||
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est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/> | est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/>nnSynonyms:zbi-29::Tn10 | ||
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zca-1230::Tn10 | zca-1230::Tn10 | ||
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CAG18478 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39407 CGSC7356]<ref name='LIB:cgsc'/> | CAG18478 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39407 CGSC7356]<ref name='LIB:cgsc'/> | ||
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est. P1 cotransduction: 47% <ref name='LIB:Nichols_linked_Tn'/> | est. P1 cotransduction: 47% <ref name='LIB:Nichols_linked_Tn'/>nnSynonyms:zbj-1230::Tn10 | ||
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===Notes=== | ===Notes=== | ||
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]]) | [https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]]) | ||
| | | | ||
[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0003009&formSubmitReturn=1 ASAP:ABE-0003009] | [http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0003009&formSubmitReturn=1 ASAP:ABE-0003009] | ||
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<noinclude><protect> | <noinclude><protect> | ||
==References== | ==References== | ||
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Revision as of 18:44, 21 January 2009
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Alleles and Phenotypes | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
aat |
|---|---|
| Mnemonic | |
| Synonyms | |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
19.96 minutes |
MG1655: 926655..925951 |
||
|
W3110 |
|
W3110: 927854..927150 |
||
| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature type | Location | Description |
|---|---|---|
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
Δaat (Keio:JW0868) |
deletion |
deletion |
PMID:16738554[3] | ||||
|
aat-1 |
Other |
MS845 has only 50% the tryptophanase activity of parent strain. |
PMID:137233[5] | ||||
|
Δaat-784::kan |
PMID:16738554[3] | ||||||
|
aat-1 |
Cell Shape |
Cells appear as clubs,spheres, or ellipsoids. Normal rod-shaped morphology was restored by addition of D-alanine or D-glutamic acid to the medium. |
PMID:137233[5] | ||||
|
aat-1 |
Growth Phenotype |
When aspartate was the sole nitrogen source, the 'aat-1' mutant strain (MS845 F'128) grew about twice as fast as its parent (doubling times of 2.3 and 4.8 h, respectively). |
PMID:137233[5] |
| |||
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0868 |
Plasmid clone |
PMID:16769691[6]
Status:Clone OK Primer 1:GCCCGCCTGGTTCAGCTTTCTCG Primer 2:CCTTCTTGTGGTGAAAACAAGCA | |
|
zbh-29::Tn10 |
Linked marker |
est. P1 cotransduction: % [7]nnSynonyms:zbi-29::Tn10 | |
|
zca-1230::Tn10 |
Linked marker |
est. P1 cotransduction: 47% [7]nnSynonyms:zbj-1230::Tn10 | |
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 5.2 Deutch, CE et al. (1977) Pleiotropic phenotype of an Escherichia coli mutant lacking leucyl-, phenylalanyl-transfer ribonucleic acid-protein transferase. J. Bacteriol. 129 544-6 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).