bamA:Gene: Difference between revisions

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===Notes===
===Notes===


A consensus has been reached for renaming the genes that encode components of the outer membrane β-barrel assembly machine, (formerly known as the YaeT complex of ''E. coli'' or the Omp85 complex of ''N. meningitidis'').  The new designation for this multi-component structure is the Bam complex (for [[b]]eta-barrel [[a]]ssembly [[m]]achine).  
A consensus has been reached for renaming the genes that encode components of the outer membrane β-barrel assembly machine, (formerly known as the YaeT complex of ''E. coli'' or the Omp85 complex of ''N. meningitidis'').  The new designation for this multi-component structure is the Bam complex (for '''b'''eta-barrel '''a'''ssembly ''' m'''achine).  


The Bam complex in ''E. coli'' consists of BamA (YaeT), BamB (YfgL), BamC (NlpB), BamD (YfiO), and BamE (SmpA).  Letters have been assigned on the basis of molecular weight, from highest to lowest <ref name='LIB:EcoGene'/>.
The Bam complex in ''E. coli'' consists of BamA (YaeT), BamB (YfgL), BamC (NlpB), BamD (YfiO), and BamE (SmpA).  Letters have been assigned on the basis of molecular weight, from highest to lowest <ref name='LIB:EcoGene'/>.

Revision as of 20:11, 30 September 2007

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

yaeT

Mnemonic
Synonyms

ECK0176, b0177, JW0172, up05, ecfK, yzzN, yzzY, omp85(N.m.), bamA[1][2][3]

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Notes

A consensus has been reached for renaming the genes that encode components of the outer membrane β-barrel assembly machine, (formerly known as the YaeT complex of E. coli or the Omp85 complex of N. meningitidis). The new designation for this multi-component structure is the Bam complex (for beta-barrel assembly machine).

The Bam complex in E. coli consists of BamA (YaeT), BamB (YfgL), BamC (NlpB), BamD (YfiO), and BamE (SmpA). Letters have been assigned on the basis of molecular weight, from highest to lowest [3].

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

4.27 minutes 

MG1655: 197928..200360
gbrowse_img.php?coord=196928..201360&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 197928..200360
gbrowse_img.php?coord=196928..201360&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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Feature type Location Description
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Notes

Alleles and Phenotypes

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Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

yaeT6

in-frame insertion of Gln–Lys after amino acid 218 of YaeT (BamA)

Sensitivity to

Rifampicin, Erythromycin, Novobiocin, Bacitracin

[4]

Suppressor of imp4213 sensitivity to deoxycholate and chenodeoxycholate

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Notes

Genetic Resources

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Resource Resource Type Source Notes/Reference

JW0172

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCGCGATGAAAAAGTTGCTCAT

Primer 2:CCCCAGGTTTTACCGATGTTAAA

zad-220::Tn10

Linked marker

CAG12025 = CGSC7326[6]

est. P1 cotransduction: 10% [7]

yafC502::Tn10

Linked marker

CAG18436 = CGSC7328[6]

est. P1 cotransduction: 25% [7]

Synonyms:zae-502::Tn10

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Notes

Accessions in Other Databases

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Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:G6093

EchoBASE (EcoliWiki Page)

EchoBASE:EB2541

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0177

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0214

EcoGene (EcoliWiki Page)

EcoGene:EG12676

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:YAET

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Notes

Links

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Name URL Comments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  4. ↑ Ruiz, N et al. (2006) Probing the barrier function of the outer membrane with chemical conditionality. ACS Chem. Biol. 1 385-95 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).


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