bamC:Gene: Difference between revisions

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!{{table_heading_style}} |Synonyms
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''ECK2473, b2477, JW2462, dapX, bamC''<ref name='LIB:Riley_2006'/><ref name='LIB:EcoGene'/>
''ECK2473, b2477, JW2462, dapX, bamC '' <ref name='LIB:Riley_2006'/><ref name='LIB:EcoGene'/>
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Revision as of 18:48, 30 September 2007

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

nlpB

Mnemonic
Synonyms

ECK2473, b2477, JW2462, dapX, bamC [1][2]

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

55.95 minutes 

MG1655: 2596887..2595853
gbrowse_img.php?coord=2594853..2597887&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 2597521..2596487
gbrowse_img.php?coord=2595487..2598521&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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Feature type Location Description
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Notes

Alleles and Phenotypes

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Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔnlpB (Keio:JW2462)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC9964[4]

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Notes

Genetic Resources

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Resource Resource Type Source Notes/Reference

JW2462

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCGCTTACTCTGTTCAAAAGTC

Primer 2:CCtTTGCTAAACGCAGCCTGGAA

purC80::Tn10

Linked marker

CAG18470 = CGSC7413[4]

est. P1 cotransduction: 93% [6]

zfh-208::Tn10

Linked marker

CAG18481 = CGSC7417[4]

est. P1 cotransduction: 2% [6]

Synonyms:zff-208::Tn10

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Notes

Accessions in Other Databases

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Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10658

EchoBASE (EcoliWiki Page)

EchoBASE:EB0652

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2477

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3005

EcoGene (EcoliWiki Page)

EcoGene:EG10658

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:NLPB

CGSC (EcoliWiki Page)

CGSC:33030

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Notes

NlpB is not essential and demonstrates very subtle outer membrane defects. nlpB- cells affect the stability of the multiprotein YaeT (Bam) complex in vitro [7],[8].

Links

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Name URL Comments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  3. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Malinverni, JC et al. (2006) YfiO stabilizes the YaeT complex and is essential for outer membrane protein assembly in Escherichia coli. Mol. Microbiol. 61 151-64 PubMed
  8. ↑ Sklar, JG et al. (2007) Lipoprotein SmpA is a component of the YaeT complex that assembles outer membrane proteins in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 104 6400-5 PubMed


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