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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10163 ECOCYC:EG10163]
EcoCyc:EG10163
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG10163 EcoGene:EG10163]
EcoGene:EG10163
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RegulonDB:ECK120000159
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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EchoBASE:EB0161
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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ASAP:ABE-0012772
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Revision as of 07:33, 27 May 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

cpxA

Mnemonic

Conjugative plasmid expression

Synonyms

ECK3904, b3911, JW3882, ecfB, eup, ssd, rssE, ecf[1][2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

88.4 minutes 

MG1655: 4102998..4101625
<gbrowseImage> name=NC_000913:4101625..4102998 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4084457..4083084
<gbrowseImage> name=NC_012967:4083084..4084457 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3991294..3992667
<gbrowseImage> name=NC_012759:3991294..3992667 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3531706..3533079
<gbrowseImage> name=AC_000091:3531706..3533079 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4202695..4201322
<gbrowseImage> name=NC_010473:4201322..4202695 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔcpxA (Keio:JW3882)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10799[4]

cpxA2(ts)

temperature sensitive

PMID:6998969[5]

CGSC:10943

ΔcpxA771::kan

PMID:16738554[3]

CGSC:100386

cpxA2

Resistant to

Resistance to Amikacin

PMID:2185221[6]

Strain: AE2038

Figure 1

ecfB1

Resistant to

Resistance to 90 micrograms/ml Kanamycin

PMID:353502[7]

Strain: GE444

See table 2.

cpxA in strains KEC1 - KEC30

Carbon Utilization

Carbon utilization of L-Serine

PMID:6802799[8]

  • Parent strain: CU1008
    • Strain: KEC1 - KEC20
  • Parent Strain: W4977
    • Strain: KEC21-KEC30

See table one and Results section

ssd in mutant strain KEC3

Resistant to

Resistant to Kanamycin

PMID:6802799[8]

Strain: KEC3

see table 2

ssd in mutant strain KEC3

Resistant to

Resistant to Neomycin

PMID:6802799[8]

Strain: KEC3

Table 2

ecfB1 kanA10

Resistant to

Resistance to Aminoglycoside Kanamycin

PMID:353502[7]

Strain: GE442

Parental: AB663

See details for subclass A for Kanomycin here

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3882

Plasmid clone

Shigen

PMID:16769691[9]

Status:Clone OK

Primer 1:GCCATAGGCAGCTTAACCGCGCG

Primer 2:CCACTCCGCTTATACAGCGGCAA

8B10

Kohara Phage

Genobase

PMID:3038334[10]

4B6

Kohara Phage

Genobase

PMID:3038334[10]

zih-35::Tn10

Linked marker

CAG18495 = CGSC7467[4]

est. P1 cotransduction: 21% [11]

pflD501::Tn10

Linked marker

CAG18477 = CGSC7470[4]

est. P1 cotransduction: 17% [11]
Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10163

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10163

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948405

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000159

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0161

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012772

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ McEwen, J & Silverman, P (1980) Genetic analysis of Escherichia coli K-12 chromosomal mutants defective in expression of F-plasmid functions: identification of genes cpxA and cpxB. J. Bacteriol. 144 60-7 PubMed
  6. ↑ Rainwater, S & Silverman, PM (1990) The Cpx proteins of Escherichia coli K-12: evidence that cpxA, ecfB, ssd, and eup mutations all identify the same gene. J. Bacteriol. 172 2456-61 PubMed
  7. ↑ 7.0 7.1 Thorbjarnardóttir, SH et al. (1978) Mutations determining generalized resistance to aminoglycoside antibiotics in Escherichia coli. Mol. Gen. Genet. 161 89-98 PubMed
  8. ↑ 8.0 8.1 8.2 Newman, EB et al. (1982) L-serine degradation in Escherichia coli K-12: directly isolated ssd mutants and their intragenic revertants. J. Bacteriol. 150 710-5 PubMed
  9. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  10. ↑ 10.0 10.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  11. ↑ 11.0 11.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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