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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11112 ECOCYC:EG11112]
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG11112 EcoGene:EG11112]
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Escherichia coli str. K-12 substr. MG1655  
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RegulonDB:ECK120001098
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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EchoBASE:EB1103
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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ASAP:ABE-0003009
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Revision as of 07:15, 27 May 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

aat

Mnemonic

Amino acyl-tRNA-protein transferase

Synonyms

ECK0876, b0885, JW0868, ycaA, G8002[1][2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

19.96 minutes 

MG1655: 926655..925951
<gbrowseImage> name=NC_000913:925951..926655 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 944490..943786
<gbrowseImage> name=NC_012967:943786..944490 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 828919..829623
<gbrowseImage> name=NC_012759:828919..829623 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 927854..927150
<gbrowseImage> name=AC_000091:927150..927854 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 980583..979879
<gbrowseImage> name=NC_010473:979879..980583 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δaat (Keio:JW0868)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8899[4]

aat-1

MS845 has only 50% the tryptophanase activity of parent strain.

PMID:137233[5]

CGSC:8542

Δaat-784::kan

PMID:16738554[3]

CGSC:99874

aat-1

Growth Phenotype

When aspartate was the sole nitrogen source, the 'aat-1' mutant strain (MS845 F'128) grew about twice as fast as its parent (doubling times of 2.3 and 4.8 h, respectively).

PMID:137233[5]

CGSC:8542

att-1

Cell Shape

Abnormal Morphology appearing as clubs, spheres or ellipsoids.

PMID:137233[5]

Parental Strain: W4977

Experimental Strain: MS845

See table 1.

Normal rod-shaped morphology was restored by addition of D-alanine or D-glutamic acid to the medium

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0868

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCCGCCTGGTTCAGCTTTCTCG

Primer 2:CCTTCTTGTGGTGAAAACAAGCA

1H1

Kohara Phage

Genobase

PMID:3038334[7]

1F10

Kohara Phage

Genobase

PMID:3038334[7]

zbh-29::Tn10

Linked marker

CAG18493 = CGSC7353[4]

est. P1 cotransduction: % [8]
Synonyms:zbi-29::Tn10

zca-1230::Tn10

Linked marker

CAG18478 = CGSC7356[4]

est. P1 cotransduction: 47% [8]
Synonyms:zbj-1230::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11112

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11112

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945490

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001098

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1103

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003009

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 5.2 Deutch, CE et al. (1977) Pleiotropic phenotype of an Escherichia coli mutant lacking leucyl-, phenylalanyl-transfer ribonucleic acid-protein transferase. J. Bacteriol. 129 544-6 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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