tilS:Gene: Difference between revisions

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Refreshing information from GenBank record NC_000913
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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''mesJ''
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Nucleoside synthesis
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lysidine synthesis is defective in about 50% of the tRNAlle2 molecules. See figure 3B.
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PMID:14527414
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lysidine synthesis is defective in about 50% of the tRNAlle2 molecules. See figure 3B. 


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Revision as of 21:47, 24 April 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

tilS

Mnemonic

tRNA(Ile) lysidine synthetase

Synonyms

ECK0187, b0188, JW0183, mesJ, yaeN[1], yaeN

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

4.58 minutes 

MG1655: 212331..213629
<gbrowseImage> name=NC_000913:212331..213629 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 215173..216471
<gbrowseImage> name=NC_012967:215173..216471 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 212330..213628
<gbrowseImage> name=NC_012759:212330..213628 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 212331..213629
<gbrowseImage> name=AC_000091:212331..213629 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 186435..187733
<gbrowseImage> name=NC_010473:186435..187733 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

mesJ

Nucleoside synthesis

lysidine synthesis is defective in about 50% of the tRNAlle2 molecules. See figure 3B.

PMID:14527414[2]

lysidine synthesis is defective in about 50% of the tRNAlle2 molecules. See figure 3B.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0183

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCACACTCACGCTCAATAGACA

Primer 2:CCACTAAGCGTTTTCTGCCAGAC

6F5

Kohara Phage

Genobase

PMID:3038334[4]

10F9

Kohara Phage

Genobase

PMID:3038334[4]

zad-220::Tn10

Linked marker

CAG12025 = CGSC7326[5]

est. P1 cotransduction: 3% [6]

yafC502::Tn10

Linked marker

CAG18436 = CGSC7328[5]

est. P1 cotransduction: 49% [6]
Synonyms:zae-502::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

ECOCYC:G6096

Escherichia coli str. K-12 substr. MG1655

EcoGene (EcoliWiki Page)

EcoGene:EG13220

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944889

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Soma, A et al. (2003) An RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNA. Mol. Cell 12 689-98 PubMed
  3. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. ↑ 4.0 4.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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