cpxA:Gene: Difference between revisions

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est. P1 cotransduction: 17% <ref name='LIB:Nichols_linked_Tn'/> <br />  
est. P1 cotransduction: 17% <ref name='LIB:Nichols_linked_Tn'/> <br />  
Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).  
Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).  
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[[:Category:Kohara_Phage:8B10|8B10]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=540 Genobase]
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PMID:3038334


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Revision as of 21:24, 2 September 2011

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

cpxA

Mnemonic

Conjugative plasmid expression

Synonyms

ECK3904, b3911, JW3882, ecfB, eup, ssd, rssE, ecf[1][2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

88.4 minutes 

MG1655: 4102998..4101625
<gbrowseImage> name=NC_000913:4101625..4102998 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4084457..4083084
<gbrowseImage> name=NC_012967:4083084..4084457 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3991294..3992667
<gbrowseImage> name=NC_012759:3991294..3992667 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3531706..3533079
<gbrowseImage> name=AC_000091:3531706..3533079 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4202695..4201322
<gbrowseImage> name=NC_010473:4201322..4202695 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔcpxA (Keio:JW3882)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10799[4]

cpxA2(ts)

temperature sensitive

PMID:6998969[5]

CGSC:10943

ΔcpxA771::kan

PMID:16738554[3]

CGSC:100386


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3882

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCATAGGCAGCTTAACCGCGCG

Primer 2:CCACTCCGCTTATACAGCGGCAA

zih-35::Tn10

Linked marker

CAG18495 = CGSC7467[4]

est. P1 cotransduction: 21% [7]

pflD501::Tn10

Linked marker

CAG18477 = CGSC7470[4]

est. P1 cotransduction: 17% [7]
Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).

8B10

Kohara Phage

Genobase

PMID:3038334[8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10163

EchoBASE (EcoliWiki Page)

EchoBASE:EB0161

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3911

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4863

EcoGene (EcoliWiki Page)

EcoGene:EG10163

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:CPXA

CGSC (EcoliWiki Page)

CGSC:908

ASAP (EcoliWikiPage)

ASAP:ABE-0012772


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ McEwen, J & Silverman, P (1980) Genetic analysis of Escherichia coli K-12 chromosomal mutants defective in expression of F-plasmid functions: identification of genes cpxA and cpxB. J. Bacteriol. 144 60-7 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  8. ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed

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