Category:Strain:W1485: Difference between revisions
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!align=left {{table_heading_style}} |Construction | |||
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== Notes == | == Notes == | ||
W1485 was derived from E. coli K-12 (λ<sup>+</sup>) F<sup>+</sup> [wild-type] after UV treatment and selection on blood agar plates<ref name='LIB:Neidhardt1996'/>. W1485 has been cured of [[Bacteriophage_λ]] but is still [[:Category:Gene_List:Plasmid_F|F<sup>+</sup>]]. W1485 also carries the ''rph-1'' mutation.<ref>[[http://cgsc.biolhttp://cgsc.biology.yale.edu/Strain.php?ID=5225 CGSC 5024]]</ref> W1485 is the immediate parent of MG1655, W3110, and W4185E.<ref name='LIB:Neidhardt1996'/> | W1485 was derived from E. coli K-12 (λ<sup>+</sup>) F<sup>+</sup> [wild-type] after UV treatment and selection on blood agar plates<ref name='LIB:Neidhardt1996'/>. W1485 has been cured of [[Bacteriophage_λ]] but is still [[:Category:Gene_List:Plasmid_F|F<sup>+</sup>]]. W1485 also carries the ''rph-1'' mutation.<ref>[[http://cgsc.biolhttp://cgsc.biology.yale.edu/Strain.php?ID=5225 CGSC 5024]]</ref> W1485 is the immediate parent of MG1655, W3110, and W4185E.<ref name='LIB:Neidhardt1996'/> | ||
Revision as of 02:48, 2 July 2011
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Notes
W1485 was derived from E. coli K-12 (λ+) F+ [wild-type] after UV treatment and selection on blood agar plates[1]. W1485 has been cured of Bacteriophage_λ but is still F+. W1485 also carries the rph-1 mutation.[2] W1485 is the immediate parent of MG1655, W3110, and W4185E.[1]
W1485 does not contain a suppressor mutation as was described in Bachmann's 1972 review of E. coli pedigrees.[3]. In her 1996 review[1], she reported that the strain distributed by the Yanofsky lab as "W1485" does carry a suppressor mutation (supE42 = glnV42) and also carries one copy of the γδ insertion sequence. This strain, which was inadvertently used by a number of laboratories under the name W1485, has been renamed W1485E (CGSC).
Visick and Clarke[4] reported that an isolate of W1485 that they obtained from R. Maurer (RM4606) contains the rpoS936(Am) allele, which changes codon 33 from CAG to TAG. It is not known whether this mutation is present in the stock of W4185 at the [Coli Genetics Stock Center]. MG1655 has a CAG codon, W3110 has xx
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 Neidhardt, FC et al. (1996) Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology 2nd ed. (ASM Press, Washington, DC)
- ↑ [CGSC 5024]
- ↑ Bachmann, BJ (1972) Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev 36 525-57 PubMed
- ↑ Visick, JE & Clarke, S (1997) RpoS- and OxyR-independent induction of HPI catalase at stationary phase in Escherichia coli and identification of rpoS mutations in common laboratory strains. J. Bacteriol. 179 4158-63 PubMed
Subcategories
This category has the following 5 subcategories, out of 5 total.