mlrA:Gene: Difference between revisions

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Convert to gbrowse_img.php extension.
Fixing Blattner deletion strains in alleles table.
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0007030 yehV::Tn5KAN]
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0007030 yehV::Tn5KAN-I-SceI] (FB20627)
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Insertion at nt 411 in Minus orientation
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PMID:12519969
PMID:15262929
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[[asapstrains:ASAP|ASAP]]
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20627]
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At position 411 in Minus orientation, contains plasmid  [[pKD46]].<br />ASAP strain name: FB20627
contains [[pKD46]]  
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Revision as of 21:53, 9 March 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mlrA

Mnemonic

MerR-like regulator

Synonyms

ECK2119, b2127, JW2115, yehV[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

47.69 minutes, 47.69 minutes 

MG1655: 2212888..2213619
<gbrowseImage> name=NC_000913:2212888..2213619 source=ecoli preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2166063..2166794
<gbrowseImage> name=NC_012967:2166063..2166794 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2105371..2106102
<gbrowseImage> name=NC_012759:2105371..2106102 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2218200..2218931
<gbrowseImage> name=AC_000091:2218200..2218931 source=ecoli_W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2303896..2304627
<gbrowseImage> name=NC_010473:2303896..2304627 source=ecoli_DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔmlrA (Keio:JW2115)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9715[3]

yehV::Tn5KAN-I-SceI (FB20627)

Insertion at nt 411 in Minus orientation

PMID:15262929[4]

E. coli Genome Project:FB20627

contains pKD46

ΔmlrA752::kan

PMID:16738554[2]

CGSC:101878



Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2115

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCGCGCTTTACACAATTGGTGA

Primer 2:CCAATGCCGAGTGGGAAAATATC

zbd-3104::Tn10

Linked marker

CAG12021 = CGSC7344[3]

est. P1 cotransduction: 22% [6]
Synonyms:zbc-3105::Tn10, zbd-3105::Tn10

napA722::Tn10

Linked marker

CAG12098 = CGSC7400[3]

est. P1 cotransduction: % [6]
Synonyms:zeg-722, zei-722::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG12008

EchoBASE (EcoliWiki Page)

EchoBASE:EB1946

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2127

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2657

EcoGene (EcoliWiki Page)

EcoGene:EG12008

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:YEHV

ASAP (EcoliWikiPage)

ASAP:ABE-0007030



Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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