hyaC:Gene: Difference between revisions

From EcoliWiki
Jump to navigation Jump to search
Convert to gbrowse_img.php extension.
Fixing Blattner deletion strains in alleles table.
Line 215: Line 215:
|-  
|-  
|
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0003290 hyaC]
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0003290 hyaC::Tn5KAN-I-SceI] (FB20076)
|
|
 
Insertion at nt 460 in Minus orientation
|
|


Line 225: Line 225:


|
|
PMID:12519969
PMID:15262929
|
|
[[asapstrains:ASAP|ASAP]]
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20076]
|
|
At position 460 in Minus orientation, does not contain plasmid  [[pKD46]] Insertion site is approximate.<br />ASAP strain name: FB20076
does not contain [[pKD46]]  
|-  
|-  
|
|

Revision as of 21:44, 9 March 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hyaC

Mnemonic

Hydrogenase 1

Synonyms

ECK0965, b0974, JW0956, cybH[1]


Notes

Location(s) and DNA Sequence

[back to top]


See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

22.29 minutes 

MG1655: 1034289..1034996
<gbrowseImage> name=NC_000913:1034289..1034996 source=ecoli preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1052157..1052864
<gbrowseImage> name=NC_012967:1052157..1052864 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 937257..937964
<gbrowseImage> name=NC_012759:937257..937964 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1035488..1036195
<gbrowseImage> name=AC_000091:1035488..1036195 source=ecoli_W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1088217..1088924
<gbrowseImage> name=NC_010473:1088217..1088924 source=ecoli_DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes


Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔhyaC (Keio:JW0956)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC8952[3]

hyaC::Tn5KAN-I-SceI (FB20076)

Insertion at nt 460 in Minus orientation

PMID:15262929[4]

E. coli Genome Project:FB20076

does not contain pKD46

ΔhyaC788::kan

PMID:16738554[2]

CGSC:101448



Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0956

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCCAACAGAAAAGCGACAACGT

Primer 2:CCTGAACGCTCCTTGTTACTTAT

zcb-3059::Tn10

Linked marker

CAG12094 = CGSC7358[3]

est. P1 cotransduction: 28% [6]

zcc-282::Tn10

Linked marker

CAG18466 = CGSC7359[3]

est. P1 cotransduction: 34% [6]


Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10470

EchoBASE (EcoliWiki Page)

EchoBASE:EB0465

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0974

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C1115

EcoGene (EcoliWiki Page)

EcoGene:EG10470

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:HYAC

CGSC (EcoliWiki Page)

CGSC:31788

ASAP (EcoliWikiPage)

ASAP:ABE-0003290



Notes

Links

[back to top]



<protect>

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories