nusA:Gene Product(s): Difference between revisions

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Table edited by Kcarter via TableEdit
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transcription termination
transcription termination
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GO_REF:0000004
PMID:12161562
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IEA: Inferred from Electronic Annotation
IDA: Inferred from Direct Assay
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SP_KW:KW-0806
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P
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Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
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complete
|-
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|


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GO:0006355
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regulation of transcription, DNA-dependent
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GO_REF:0000004
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IEA: Inferred from Electronic Annotation
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SP_KW:KW-0805
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|
P
P
|
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
Fig.3. NusA stimulates termination. Ran products of transcription reactions on gel. Difference between -TRAP -NusA and -TRAP +NusA. +NusA has bands at the termination sites, indicating NusA is involved in transcription termination.
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complete  
complete  
Line 380: Line 361:
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Fig. 3. Gel showing transcription reactions that were carried out in the presence or absence of NusA and TRAP. NusA stimulates termination at specific sites in the genome, as can be seen on the gel (difference between -TRAP -NusA and -TRAP and +NusA)
Fig. 3. Gel showing transcription reactions that were carried out in the presence or absence of NusA and TRAP. NusA stimulates termination at specific sites in the genome, as can be seen on the gel (difference between -TRAP -NusA and -TRAP and +NusA)
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complete
|-
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GO:0006355
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regulation of transcription, DNA-dependent
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GO_REF:0000004
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IEA: Inferred from Electronic Annotation
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SP_KW:KW-0805
|
P
|
Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
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complete
|-
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GO:0006353
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transcription termination
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GO_REF:0000004
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IEA: Inferred from Electronic Annotation
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SP_KW:KW-0806
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P
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Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
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|
complete  
complete  

Revision as of 22:41, 2 May 2010

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

NusA

Synonyms

transcription termination/antitermination L factor[1], B3169[2][1], NusA[2][1]

Product description

transcription termination/antitermination L factor[2][3]

Transcription termination/antitermination L factor; mutant survives lambda induction[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference(s) Evidence Code with/from Aspect Notes Status
GO:0005737

cytoplasm

PMID:16858726[5]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [6]

complete

GO:0000166

nucleotide binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010995

F

Seeded from EcoCyc [6]

complete

GO:0003700

transcription factor activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013735

F

Seeded from EcoCyc [6]

complete

GO:0003723

RNA binding

GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR003029
InterPro:IPR004087
InterPro:IPR004088
InterPro:IPR009019
InterPro:IPR010213
SP_KW:KW-0694

F

Seeded from EcoCyc [6]

complete

GO:0005515

protein binding

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010995
InterPro:IPR013735

F

Seeded from EcoCyc [6]

complete

GO:0006350

transcription<input name="field[2]" type="hidden" value="transcription" />

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc [6]

complete

GO:0006353

transcription termination

PMID:12161562[7]

IDA: Inferred from Direct Assay

P

Fig.3. NusA stimulates termination. Ran products of transcription reactions on gel. Difference between -TRAP -NusA and -TRAP +NusA. +NusA has bands at the termination sites, indicating NusA is involved in transcription termination.

complete

GO:0006950

response to stress

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0346

P

Seeded from EcoCyc [6]

complete

GO:0030528

transcription regulator activity

PMID:12161562[7]

IDA: Inferred from Direct Assay

F

Fig.2. NusA stimulates pausing of the RNA polymerase at specific sites. Can see on the gel, bands at specific nucleotide sites larger when NusA present.

complete

GO:0031554

regulation of transcription termination

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013735

P

Seeded from EcoCyc [6]

complete

GO:0031564

transcription antitermination

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0889

P

Seeded from EcoCyc [6]

complete

GO:0043565

sequence-specific DNA binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003583

F

Seeded from EcoCyc [6]

complete

GO:0005622

intracellular

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003583

C

Seeded from EcoCyc [6]

complete

GO:0030528

transcription regulator activity

GO_REF:0000002
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010213
InterPro:IPR010214

F

Seeded from EcoCyc [6]

complete

GO:0031554

regulation of transcription termination

PMID:12161562[7]

IDA: Inferred from Direct Assay

P

Fig. 3. Gel showing transcription reactions that were carried out in the presence or absence of NusA and TRAP. NusA stimulates termination at specific sites in the genome, as can be seen on the gel (difference between -TRAP -NusA and -TRAP and +NusA)

complete

GO:0006355

regulation of transcription, DNA-dependent

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0805

P

Seeded from EcoCyc [6]

complete

GO:0006353

transcription termination

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0806

P

Seeded from EcoCyc [6]

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

prsA

PMID:15690043[8]

Experiment(s):EBI-886823

Protein

rpsD

PMID:15690043[8]

Experiment(s):EBI-886823

Protein

rpsG

PMID:15690043[8]

Experiment(s):EBI-886823

Protein

tufA

PMID:15690043[8]

Experiment(s):EBI-886823

Protein

infB

PMID:15690043[8]

Experiment(s):EBI-886823, EBI-893117

Protein

rplA

PMID:15690043[8]

Experiment(s):EBI-886823

Protein

rplB

PMID:15690043[8]

Experiment(s):EBI-886823

Protein

rplC

PMID:15690043[8]

Experiment(s):EBI-886823

Protein

rplD

PMID:15690043[8]

Experiment(s):EBI-886823

Protein

rplO

PMID:15690043[8]

Experiment(s):EBI-886823

Protein

rplV

PMID:15690043[8]

Experiment(s):EBI-886823, EBI-893117

Protein

rpoA

PMID:15690043[8]

Experiment(s):EBI-886823, EBI-879354, EBI-883271, EBI-893117

Protein

rpoB

PMID:15690043[8]

Experiment(s):EBI-886823, EBI-879304, EBI-883170, EBI-893117

Protein

rpoC

PMID:15690043[8]

Experiment(s):EBI-886823, EBI-879261, EBI-893117

Protein

rpoH

PMID:15690043[8]

Experiment(s):EBI-886823

Protein

rpsC

PMID:15690043[8]

Experiment(s):EBI-886823

Protein

aceE

PMID:15690043[8]

Experiment(s):EBI-893117

Protein

metJ

PMID:15690043[8]

Experiment(s):EBI-893117

Protein

pstS

PMID:15690043[8]

Experiment(s):EBI-893117

Protein

rplS

PMID:15690043[8]

Experiment(s):EBI-893117

Protein

rplX

PMID:15690043[8]

Experiment(s):EBI-893117

Protein

rpsE

PMID:15690043[8]

Experiment(s):EBI-893117

Protein

uspA

PMID:15690043[8]

Experiment(s):EBI-893117

Protein

uvrA

PMID:15690043[8]

Experiment(s):EBI-893117

Protein

infB

PMID:16606699[9]

Experiment(s):EBI-1145014

Protein

rplS

PMID:16606699[9]

Experiment(s):EBI-1145014

Protein

rpoB

PMID:16606699[9]

Experiment(s):EBI-1145014

Protein

rplE

PMID:16606699[9]

Experiment(s):EBI-1145014

Protein

rpsD

PMID:16606699[9]

Experiment(s):EBI-1145014

Protein

rpsC

PMID:16606699[9]

Experiment(s):EBI-1145014

Protein

rpsE

PMID:16606699[9]

Experiment(s):EBI-1145014

Protein

cybC

PMID:16606699[9]

Experiment(s):EBI-1145014

Protein

fur

PMID:16606699[9]

Experiment(s):EBI-1145014

Protein

rpsG

PMID:16606699[9]

Experiment(s):EBI-1145014

Protein

rpoC

PMID:16606699[9]

Experiment(s):EBI-1145014

Protein

rhsD

PMID:16606699[9]

Experiment(s):EBI-1145014

Protein

rpsI

PMID:16606699[9]

Experiment(s):EBI-1145014

Protein

argG

PMID:16606699[9]

Experiment(s):EBI-1145014

Protein

ycgL

PMID:16606699[9]

Experiment(s):EBI-1145014

Protein

rplP

PMID:16606699[9]

Experiment(s):EBI-1145014

Protein

rplO

PMID:16606699[9]

Experiment(s):EBI-1145014

Protein

rplB

PMID:16606699[9]

Experiment(s):EBI-1145014

Protein

rplF

PMID:16606699[9]

Experiment(s):EBI-1145014
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Source Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MNKEILAVVE AVSNEKALPR EKIFEALESA LATATKKKYE QEIDVRVQID RKSGDFDTFR
RWLVVDEVTQ PTKEITLEAA RYEDESLNLG DYVEDQIESV TFDRITTQTA KQVIVQKVRE
AERAMVVDQF REHEGEIITG VVKKVNRDNI SLDLGNNAEA VILREDMLPR ENFRPGDRVR
GVLYSVRPEA RGAQLFVTRS KPEMLIELFR IEVPEIGEEV IEIKAAARDP GSRAKIAVKT
NDKRIDPVGA CVGMRGARVQ AVSTELGGER IDIVLWDDNP AQFVINAMAP ADVASIVVDE
DKHTMDIAVE AGNLAQAIGR NGQNVRLASQ LSGWELNVMT VDDLQAKHQA EAHAAIDTFT
KYLDIDEDFA TVLVEEGFST LEELAYVPMK ELLEIEGLDE PTVEALRERA KNALATIAQA
QEESLGDNKP ADDLLNLEGV DRDLAFKLAA RGVCTLEDLA EQGIDDLADI EGLTDEKAGA
LIMAARNICW FGDEA
Length

495

Mol. Wt

54.87 kDa

pI

4.4 (calculated)

Extinction coefficient

30,940 - 31,315 (calc based on 6 Y, 4 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=nusA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10665-MONOMER

SwissModel (EcoliWiki Page)

SwissModel:P0AFF6

UniProt (EcoliWiki Page)

UniProt:P0AFF6

PFAM (EcoliWiki Page)

PFAM:PF00575

RefSeq (EcoliWiki Page)

RefSeq:NP_417638


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
  6. ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 6.10 6.11 6.12 6.13 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  7. ↑ 7.0 7.1 7.2 Yakhnin, AV & Babitzke, P (2002) NusA-stimulated RNA polymerase pausing and termination participates in the Bacillus subtilis trp operon attenuation mechanism invitro. Proc Natl Acad Sci U S A 99 11067-72 PubMed
  8. ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 8.11 8.12 8.13 8.14 8.15 8.16 8.17 8.18 8.19 8.20 8.21 8.22 8.23 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  9. ↑ 9.00 9.01 9.02 9.03 9.04 9.05 9.06 9.07 9.08 9.09 9.10 9.11 9.12 9.13 9.14 9.15 9.16 9.17 9.18 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed

Categories

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