tsf:Gene: Difference between revisions

From EcoliWiki
Jump to navigation Jump to search
← Older editNewer edit →
Data loaded by TableEdit_Loader.
Automated update of mnemonics from EcoGene.
Line 34: Line 34:


|-
|-
!align=left |Standard name
!align=left {{table_heading_style}} |Standard name
||
||
''tsf''  
''tsf''
|-
|-
!align=left |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
||
||
 
Translation stable factor
|-
|-
!align=left |Synonyms
!align=left {{table_heading_style}} |Synonyms
||
||
''ECK0169, b0170, JW0165''<ref name='LIB:Riley_2006'/>
''ECK0169, b0170, JW0165''<ref name='LIB:Riley_2006'/>
Line 66: Line 66:
****************************************************************************************** -->{{Gene_location_help}}
****************************************************************************************** -->{{Gene_location_help}}
{|  id="F46a9aa0093b80"  class=" tableEdit Gene_location_table"   
{|  id="F46a9aa0093b80"  class=" tableEdit Gene_location_table"   
|-
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-  
|-  
Line 135: Line 135:
****************************************************************************************** -->{{Gene_sequence_features_help}}
****************************************************************************************** -->{{Gene_sequence_features_help}}
{|  id="R46a9aa013c7b2"  class=" tableEdit Gene_sequence_features_table"   
{|  id="R46a9aa013c7b2"  class=" tableEdit Gene_sequence_features_table"   
|-
|- {{table_heading_style}}
!|Feature type!!Location!!Description
!|Feature type!!Location!!Description


Line 178: Line 178:
|-  
|-  
|
|
tsfK24A
tsfH148A
|
|


|
|
K24A
H148A
|
|


|
|
No change in binding to EF-Tu and in promoting GDP exchange
At least 100-fold decrease in affinity between EF-Ts and EF-Tu and only small amount of GDP exchange activity
|
|


Line 195: Line 195:
|-  
|-  
|
|
tsfD81A
tsfK24A
|
|


|
|
D81A
K24A
|
|


|
|
2 to 3-fold less active in promoting GDP exchange and slightly lower binding constant for interaction with EF- Tu
No change in binding to EF-Tu and in promoting GDP exchange
|
|


Line 212: Line 212:
|-  
|-  
|
|
tsfH148A
tsfD81A
|
|


|
|
H148A
D81A
|
|


|
|
At least 100-fold decrease in affinity between EF-Ts and EF-Tu and only small amount of GDP exchange activity
2 to 3-fold less active in promoting GDP exchange and slightly lower binding constant for interaction with EF- Tu
|
|


Line 247: Line 247:
****************************************************************************************** -->{{Gene_resources_help}}
****************************************************************************************** -->{{Gene_resources_help}}
{|  id="N46a9aa022625c"  class=" tableEdit Gene_resource_table"   
{|  id="N46a9aa022625c"  class=" tableEdit Gene_resource_table"   
|-
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
!|Resource!!Resource Type!!Source!!Notes/Reference
|-  
|-  
Line 303: Line 303:
****************************************************************************************** -->{{Gene_accessions_help}}
****************************************************************************************** -->{{Gene_accessions_help}}
{|  id="L46a9aa02aff0a"  class=" tableEdit Gene_accessions_table"   
{|  id="L46a9aa02aff0a"  class=" tableEdit Gene_accessions_table"   
|-
|- {{table_heading_style}}
!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
Line 381: Line 381:
****************************************************************************************** -->
****************************************************************************************** -->
{|  id="Y46a6773ae9c8e"  class=" tableEdit Links_table"   
{|  id="Y46a6773ae9c8e"  class=" tableEdit Links_table"   
|-
|- {{table_heading_style}}
!|Name!!URL!!Comments
!|Name!!URL!!Comments


Revision as of 21:50, 25 February 2010

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

tsf

Mnemonic

Translation stable factor

Synonyms

ECK0169, b0170, JW0165[1]

edit table


Notes

Location(s) and DNA Sequence

[back to top]


See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

4.11 minutes 

MG1655: 190857..191708
gbrowse_img.php?coord=190857..191708&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 193699..194550
gbrowse_img.php?coord=193699..194550&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 190857..191708
gbrowse_img.php?coord=190857..191708&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 164961..165812
gbrowse_img.php?coord=164961..165812&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

edit table


Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
edit table


Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

tsfF82A

F82A

2 to 3-fold less active in promoting GDP exchange and 6-fold less active in binding to EF-Tu

seeded from UniProt:P0A6P1

tsfH148A

H148A

At least 100-fold decrease in affinity between EF-Ts and EF-Tu and only small amount of GDP exchange activity

seeded from UniProt:P0A6P1

tsfK24A

K24A

No change in binding to EF-Tu and in promoting GDP exchange

seeded from UniProt:P0A6P1

tsfD81A

D81A

2 to 3-fold less active in promoting GDP exchange and slightly lower binding constant for interaction with EF- Tu

seeded from UniProt:P0A6P1

edit table


Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0165

Plasmid clone

Shigen

PMID:16769691[2]

Status:Clone OK

Primer 1:GCCGCTGAAATTACCGCATCCCT

Primer 2:CCAGACTGCTTGGACATCGCAGC

zad-220::Tn10

Linked marker

CAG12025 = CGSC7326[3]

est. P1 cotransduction: 16% [4]

yafC502::Tn10

Linked marker

CAG18436 = CGSC7328[3]

est. P1 cotransduction: 17% [4]
Synonyms:zae-502::Tn10

edit table


Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11033

EchoBASE (EcoliWiki Page)

EchoBASE:EB1026

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0170

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0206

EcoGene (EcoliWiki Page)

EcoGene:EG11033

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:TSF

CGSC (EcoliWiki Page)

CGSC:64

ASAP (EcoliWikiPage)

ASAP:ABE-0000579


edit table


Notes

Links

[back to top]



<protect>

References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  3. ↑ 3.0 3.1 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

Retrieved from "https://ecoliwiki.org/colipedia/index.php?title=tsf:Gene&oldid=1057546"