rhaS:Gene: Difference between revisions

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37% of wild-type activity
37% of wild-type activity
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seeded from [http://www.uniprot.org/uniprot/P09377 UniProt:P09377]
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rhaSH253A
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H253A
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48% of wild-type activity
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rhaSH253A
rhaSH253Q
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H253A
H253Q
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48% of wild-type activity
32% of wild-type activity
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Revision as of 17:55, 18 February 2010

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rhaS

Mnemonic
Synonyms

ECK3898, b3905, JW3876, rhaC, rhaC2[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

88.28 minutes 

MG1655: 4095759..4096595
gbrowse_img.php?coord=4095759..4096595&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 4077218..4078054
gbrowse_img.php?coord=4077218..4078054&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 3538945..3538109
gbrowse_img.php?coord=3538109..3538945&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 4195456..4196292
gbrowse_img.php?coord=4195456..4196292&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔrhaS (Keio:JW3876)

deletion

deletion

PMID:16738554[2]

Shigen

rhaSR202A

R202A

4% of wild-type activity

seeded from UniProt:P09377

rhaSS249G

S249G

93% of wild-type activity

seeded from UniProt:P09377

rhaSD250A

D250A

8.3% of wild-type activity

seeded from UniProt:P09377

rhaSS251G

S251G

87% of wild-type activity

seeded from UniProt:P09377

rhaSF248V

F248V

3.3% of wild-type activity

seeded from UniProt:P09377

rhaSL201A

L201A

48% of wild-type activity

seeded from UniProt:P09377

rhaSF248A

F248A

0.9% of wild-type activity

seeded from UniProt:P09377

rhaSN252H

N252H

0.4% of wild-type activity

seeded from UniProt:P09377

rhaSN252I

N252I

0.3% of wild-type activity

seeded from UniProt:P09377

rhaSN252S

N252S

37% of wild-type activity

seeded from UniProt:P09377

rhaSH253A

H253A

48% of wild-type activity

seeded from UniProt:P09377

rhaSN252A

N252A

31% of wild-type activity

seeded from UniProt:P09377

rhaSH205A

H205A

28% of wild-type activity

seeded from UniProt:P09377

rhaSR206A

R206A

0.1% of wild-type activity

seeded from UniProt:P09377

rhaSC246A

C246A

66% of wild-type activity

seeded from UniProt:P09377

rhaSG247A

G247A

6.2% of wild-type activity

seeded from UniProt:P09377

rhaSG247R

G247R

0.9% of wild-type activity

seeded from UniProt:P09377

xx

xx

xx

Growth Phenotype

xx

rhaSH253Q

H253Q

32% of wild-type activity

seeded from UniProt:P09377

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3876

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCACCGTATTACATAGTGTGGA

Primer 2:CCTTGCAGAAAGCCATCCCGTCC

zih-35::Tn10

Linked marker

CAG18495 = CGSC7467[4]

est. P1 cotransduction: 29% [5]

pflD501::Tn10

Linked marker

CAG18477 = CGSC7470[4]

est. P1 cotransduction: 12% [5]
Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10843

EchoBASE (EcoliWiki Page)

EchoBASE:EB0836

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3905

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4855

EcoGene (EcoliWiki Page)

EcoGene:EG10843

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:RHAS

CGSC (EcoliWiki Page)

CGSC:17950

ASAP (EcoliWikiPage)

ASAP:ABE-0012745


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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