rhaS:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
rhaS |
|---|---|
| Mnemonic |
Rhamnose |
| Synonyms |
ECK3898, b3905, JW3876, rhaC, rhaC2[1], rhaC2 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
88.28 minutes |
MG1655: 4095759..4096595 |
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NC_012967: 4077218..4078054 |
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NC_012759: 3985428..3986264 |
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W3110 |
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W3110: 3538945..3538109 |
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DH10B: 4195456..4196292 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔrhaS (Keio:JW3876) |
deletion |
deletion |
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rhaSR202A |
R202A |
4% of wild-type activity |
seeded from UniProt:P09377 | ||||
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rhaSS255A |
S255A |
80% of wild-type activity |
seeded from UniProt:P09377 | ||||
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rhaST256A |
T256A |
61% of wild-type activity |
seeded from UniProt:P09377 | ||||
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rhaSS249G |
S249G |
93% of wild-type activity |
seeded from UniProt:P09377 | ||||
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rhaSD250A |
D250A |
8.3% of wild-type activity |
seeded from UniProt:P09377 | ||||
|
rhaSS251G |
S251G |
87% of wild-type activity |
seeded from UniProt:P09377 | ||||
|
rhaSF248V |
F248V |
3.3% of wild-type activity |
seeded from UniProt:P09377 | ||||
|
rhaSF254A |
F254A |
1.2% of wild-type activity |
seeded from UniProt:P09377 | ||||
|
rhaSL201A |
L201A |
48% of wild-type activity |
seeded from UniProt:P09377 | ||||
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rhaSF248A |
F248A |
0.9% of wild-type activity |
seeded from UniProt:P09377 | ||||
|
rhaSN252H |
N252H |
0.4% of wild-type activity |
seeded from UniProt:P09377 | ||||
|
rhaSN252I |
N252I |
0.3% of wild-type activity |
seeded from UniProt:P09377 | ||||
|
rhaSN252S |
N252S |
37% of wild-type activity |
seeded from UniProt:P09377 | ||||
|
rhaSH253A |
H253A |
48% of wild-type activity |
seeded from UniProt:P09377 | ||||
|
rhaSH253Q |
H253Q |
32% of wild-type activity |
seeded from UniProt:P09377 | ||||
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rhaSN252A |
N252A |
31% of wild-type activity |
seeded from UniProt:P09377 | ||||
|
rhaSH205A |
H205A |
28% of wild-type activity |
seeded from UniProt:P09377 | ||||
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rhaSR206A |
R206A |
0.1% of wild-type activity |
seeded from UniProt:P09377 | ||||
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rhaSC246A |
C246A |
66% of wild-type activity |
seeded from UniProt:P09377 | ||||
|
rhaSG247A |
G247A |
6.2% of wild-type activity |
seeded from UniProt:P09377 | ||||
|
rhaSG247R |
G247R |
0.9% of wild-type activity |
seeded from UniProt:P09377 | ||||
|
xx |
xx |
xx |
Growth Phenotype |
xx |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3876 |
Plasmid clone |
Status:Clone OK Primer 1:GCCACCGTATTACATAGTGTGGA Primer 2:CCTTGCAGAAAGCCATCCCGTCC | |
|
Kohara Phage |
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|
Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 29% [6] | ||
|
Linked marker |
est. P1 cotransduction: 12% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 4.0 4.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
