rpsU:Gene: Difference between revisions

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===Notes===
===Notes===
The Keio collection<ref name='PMID:16738554'/> lists a deletion of ''alaS''.  The insertion in this strain is a duplication of the ''alaS'' region.<ref name='PMID:20029369'/>
The Keio collection<ref name='PMID:16738554'/> lists a deletion of ''rpsU''.  The insertion in this strain is a duplication of the ''rpsU'' region.<ref name='PMID:20029369'/>


==Genetic Resources==
==Genetic Resources==

Revision as of 23:41, 11 February 2010

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Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rpsU

Mnemonic
Synonyms

ECK3055, b3065, JW3037[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

69.16 minutes 

MG1655: 3208803..3209018
gbrowse_img.php?coord=3208803..3209018&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 3143598..3143813
gbrowse_img.php?coord=3143598..3143813&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 3209437..3209652
gbrowse_img.php?coord=3209437..3209652&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 3306548..3306763
gbrowse_img.php?coord=3306548..3306763&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔrpsU (Keio:JW3037)

deletion

deletion

PMID:16738554[2]

Shigen



Notes

The Keio collection[2] lists a deletion of rpsU. The insertion in this strain is a duplication of the rpsU region.[3]

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3037

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCCCGGTAATTAAAGTACGTGA

Primer 2:CCGTACAGACGAGTGCGGCGTGC

tolC210::Tn10

Linked marker

CAG12184 = CGSC7437[5]

est. P1 cotransduction: 30% [6]

aer-3075::Tn10

Linked marker

CAG12152 = CGSC7438[5]

est. P1 cotransduction: 80% [6]
Synonyms:zgh-3075::Tn10, zgj-3075::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10920

EchoBASE (EcoliWiki Page)

EchoBASE:EB0913

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3065

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3816

EcoGene (EcoliWiki Page)

EcoGene:EG10920

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:RPSU

CGSC (EcoliWiki Page)

CGSC:211

ASAP (EcoliWikiPage)

ASAP:ABE-0010063



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ Yamamoto, N et al. (2009) Update on the Keio collection of Escherichia coli single-gene deletion mutants. Mol. Syst. Biol. 5 335 PubMed
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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