pgm:Gene: Difference between revisions

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!align=left |Standard name
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''pgm''  
''pgm''
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!align=left |Mnemonic
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Phophoglucomutase
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!align=left |Synonyms
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''ECK0676, b0688, JW0675, blu''<ref name='LIB:Riley_2006'/>
''ECK0676, b0688, JW0675, blu''<ref name='LIB:Riley_2006'/>
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!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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!|Feature type!!Location!!Description
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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Revision as of 21:33, 25 February 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

pgm

Mnemonic

Phophoglucomutase

Synonyms

ECK0676, b0688, JW0675, blu[1]

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Notes

Cloned and sequenced by Lu and Kleckner[2]

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

15.36 minutes 

MG1655: 712781..714421
gbrowse_img.php?coord=712781..714421&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 695653..697293
gbrowse_img.php?coord=695653..697293&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 713980..715620
gbrowse_img.php?coord=713980..715620&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 765373..767013
gbrowse_img.php?coord=765373..767013&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δpgm (Keio:JW0675)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8761[4]

pgm-1

CGSC:6535

pgm-67

CGSC:6538

pgm-3

CGSC:14474

pgm-191

CGSC:14476

Δpgm-736::kan

PMID:16738554[3]

CGSC:102312


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0675

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCGCAATCCACAATCGTGCAGG

Primer 2:CCCGCGTTTTTCAGAACTTCGCT

asnB3057::Tn10

Linked marker

CAG18433 = CGSC7349[4]

est. P1 cotransduction: 66% [6]
Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.

nadA57::Tn10

Linked marker

CAG12147 = CGSC7351[4]

est. P1 cotransduction: 2% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG12144

EchoBASE (EcoliWiki Page)

EchoBASE:EB2065

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0688

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0775

EcoGene (EcoliWiki Page)

EcoGene:EG12144

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:PGM

CGSC (EcoliWiki Page)

CGSC:406

ASAP (EcoliWikiPage)

ASAP:ABE-0002345


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Lu, M & Kleckner, N (1994) Molecular cloning and characterization of the pgm gene encoding phosphoglucomutase of Escherichia coli. J. Bacteriol. 176 5847-51 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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