dnaX:Expression: Difference between revisions

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==Translation and Regulation of Translation==
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===Notes===
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Revision as of 21:38, 11 February 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Overview Transcription Translation Turnover Experimental Links References Suggestions

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
dnaX encodes two gene products from the same gene. A programmed frameshift is needed to generate the gamma subunit[1][2][3].

The amount of gamma is estimated to be about 20 molecules per cell, based on an estimate of 10 dimers[4]. However, while gamma purifies as a dimer, it is present as one subunit in the τ2γδδ'χψ DnaX complex.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Cellular level Units Medium Temperature °C Other (e.g. anaerobic) Growth rate Assay used Notes Reference(s)
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

dnaX

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Figure courtesy of RegulonDB


Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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gbrowse_img.php?coord=491296..491336&genome=ecoli&name=MG1655&trackset=Gene+DNA_+Protein&width=400&figure=Nterm&.png
This picture shows the sequence around the N-terminus.

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0470 (EcoliWiki Page)

NCBI GEO profiles for dnaX

microarray

GenExpDB:b0470 (EcoliWiki Page)

Summary of data for dnaX from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (491102..491347) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ10; Well:C6[5]

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Notes

Accessions Related to dnaX Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10245

EchoBASE (EcoliWiki Page)

EchoBASE:EB0241

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0470

EcoGene (EcoliWiki Page)

EcoGene:EG10245


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Tsuchihashi, Z & Kornberg, A (1990) Translational frameshifting generates the gamma subunit of DNA polymerase III holoenzyme. Proc. Natl. Acad. Sci. U.S.A. 87 2516-20 PubMed
  2. ↑ Blinkowa, AL & Walker, JR (1990) Programmed ribosomal frameshifting generates the Escherichia coli DNA polymerase III gamma subunit from within the tau subunit reading frame. Nucleic Acids Res. 18 1725-9 PubMed
  3. ↑ Flower, AM & McHenry, CS (1990) The gamma subunit of DNA polymerase III holoenzyme of Escherichia coli is produced by ribosomal frameshifting. Proc. Natl. Acad. Sci. U.S.A. 87 3713-7 PubMed
  4. ↑ Hübscher, U & Kornberg, A (1980) The dnaZ protein, the gamma subunit of DNA polymerase III holoenzyme of Escherichia coli. J. Biol. Chem. 255 11698-703 PubMed
  5. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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