copA:Gene: Difference between revisions

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!{{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Standard name
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''copA''  
''copA''  
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!{{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
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!{{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
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''ECK0478, b0484, JW0473, ybaR''<ref name='LIB:Riley_2006'/>
''ECK0478, b0484, JW0473, ybaR''<ref name='LIB:Riley_2006'/>
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MG1655
MG1655
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{{GBrowseFastaLink|ecoli|NC_000913|508099|510603|FASTA Dump}}
{{GBrowseFastaLink|ecoli|NC_000913|508099|510603|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=G6260 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=G6260 EcoCyc Sequence Dump]  
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W3110
W3110
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{{GBrowseFastaLink|ecoli_W3110|AC_000091|508099|510603|FASTA Dump}}  
{{GBrowseFastaLink|ecoli_W3110|AC_000091|508099|510603|FASTA Dump}}  
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|[[PMID:16738554]]<ref name=PMID:16738554/>
[[PMID:16738554]]<ref name=PMID:16738554/>
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=106906 CGSC8625]<ref name='LIB:cgsc'/>  
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=106906 CGSC8625]<ref name='LIB:cgsc'/>  
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|[[PMID:16738554]]<ref name=PMID:16738554/>
[[PMID:16738554]]<ref name=PMID:16738554/>
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[[cgscmutation:100374|CGSC:100374]]
[[cgscmutation:100374|CGSC:100374]]
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JW0473
JW0473
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
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|[[PMID:16769691]]<ref name=PMID:16769691/>
[[PMID:16769691]]<ref name=PMID:16769691/>


Status:Clone OK
Status:Clone OK
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Primer 2:ccTTCCTTCGGTTTAAACCGCAGCAACCGG  
Primer 2:ccTTCCTTCGGTTTAAACCGCAGCAACCGG  
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[[gsk:Gene|gsk-3055::Tn10]]
[[gsk:Gene|gsk-3055::Tn10]]
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est. P1 cotransduction: 79% <ref name='LIB:Nichols_linked_Tn'/>nnSynonyms:zbb-30-55::TnlO, zba-3055::Tn10  
est. P1 cotransduction: 79% <ref name='LIB:Nichols_linked_Tn'/>nnSynonyms:zbb-30-55::TnlO, zba-3055::Tn10  
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[[purK:Gene|purK79::Tn10]]
[[purK:Gene|purK79::Tn10]]
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est. P1 cotransduction: 15% <ref name='LIB:Nichols_linked_Tn'/>nnSynonyms:purE79::Tn10  
est. P1 cotransduction: 15% <ref name='LIB:Nichols_linked_Tn'/>nnSynonyms:purE79::Tn10  
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copA::kan
copA::kan
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CN2328
CN2328
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|[[PMID:11167016]]<ref name=PMID:11167016/>  
[[PMID:11167016]]<ref name=PMID:11167016/>  
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pCN2345
pCN2345
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copA with own promoter cloned into pBR322
copA with own promoter cloned into pBR322
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|[[PMID:11167016]]<ref name=PMID:11167016/>  
[[PMID:11167016]]<ref name=PMID:11167016/>  
 
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0001681&formSubmitReturn=1 ASAP:ABE-0001681]
[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0001681&formSubmitReturn=1 ASAP:ABE-0001681]
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Revision as of 18:43, 21 January 2009

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

copA

Mnemonic
Synonyms

ECK0478, b0484, JW0473, ybaR[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

10.95 minutes 

MG1655: 510603..508099
gbrowse_img.php?coord=507099..511603&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 510603..508099
gbrowse_img.php?coord=507099..511603&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔcopA (Keio:JW0473)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC8625[3]

ΔcopA1::kan

CGSC:77264

ΔcopA767::kan

PMID:16738554[2]

CGSC:100374


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0473

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:gccTCACAAACTATCGACCTGACCCTGGAC

Primer 2:ccTTCCTTCGGTTTAAACCGCAGCAACCGG

gsk-3055::Tn10

Linked marker

CAG12154 = CGSC7341[3]

est. P1 cotransduction: 79% [5]nnSynonyms:zbb-30-55::TnlO, zba-3055::Tn10

purK79::Tn10

Linked marker

CAG12171 = CGSC7342[3]

est. P1 cotransduction: 15% [5]nnSynonyms:purE79::Tn10

copA::kan

Strain

CN2328

PMID:11167016[6]

pCN2345

Plasmid Clone

copA with own promoter cloned into pBR322

PMID:11167016[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:G6260

EchoBASE (EcoliWiki Page)

EchoBASE:EB3035

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0484

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0604

EcoGene (EcoliWiki Page)

EcoGene:EG13246

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:YBAR

ASAP (EcoliWikiPage)

ASAP:ABE-0001681


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Notes

Links

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Name URL Comments
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References

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  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  6. ↑ 6.0 6.1 Petersen, C & Møller, LB (2000) Control of copper homeostasis in Escherichia coli by a P-type ATPase, CopA, and a MerR-like transcriptional activator, CopR. Gene 261 289-98 PubMed


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