ppc:Gene: Difference between revisions

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Primer 2:CCGCCGGTATTACGCATACCTGC  
Primer 2:CCGCCGGTATTACGCATACCTGC  
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[[pflD:Gene|pflD501::Tn10]]
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Linked marker
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CAG18477 = [http://cgsc2.biology.yale.edu/Strain.php?ID=37152 CGSC7470]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 84% <ref name='LIB:Nichols_linked_Tn'/>
Synonyms:zij-501::Tn10
CAG18477 also carries metF79(Am) (CGSC).
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[[argE:Gene|argE86::Tn10]]
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Linked marker
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CAG12185 = [http://cgsc2.biology.yale.edu/Strain.php?ID=37153 CGSC7471]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 88% <ref name='LIB:Nichols_linked_Tn'/>
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Revision as of 23:42, 28 July 2007

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

ppc

Mnemonic
Synonyms

ECK3947, b3956, JW3928, asp, glu, pepC[1]

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

89.41 minutes, 89.41 minutes 

MG1655: 4151121..4148470
gbrowse_img.php?coord=4147470..4152121&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 3483583..3486234
gbrowse_img.php?coord=3482583..3487234&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Notes

Sequence Features

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Feature type Location Description
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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Notes

Alleles and Phenotypes

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Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability

Δppc (Keio:JW3928)

deletion

deletion

PMID:16738554[2]

Shigen

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Notes

Genetic Resources

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Resource Resource Type Source Notes/Reference

JW3928

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCAACGAACAATATTCCGCATT

Primer 2:CCGCCGGTATTACGCATACCTGC

pflD501::Tn10

Linked marker

CAG18477 = CGSC7470[4]

est. P1 cotransduction: 84% [5]

Synonyms:zij-501::Tn10


CAG18477 also carries metF79(Am) (CGSC).

argE86::Tn10

Linked marker

CAG12185 = CGSC7471[4]

est. P1 cotransduction: 88% [5]

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Notes

Accessions in Other Databases

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Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10756

EchoBASE (EcoliWiki Page)

EchoBASE:EB0749

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3956

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4915

EcoGene (EcoliWiki Page)

EcoGene:EG10756

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:PPC

CGSC (EcoliWiki Page)

CGSC:368

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Notes

Links

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Name URL Comments
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See Help:Links_table for how to enter or edit information in this section of EcoliWiki.

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).