ispE:Gene: Difference between revisions

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Primer 2:CCAAGCATGGCTCTGTGCAATGG  
Primer 2:CCAAGCATGGCTCTGTGCAATGG  
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[[:Category:Kohara_Phage:12A3|12A3]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=246 Genobase]
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PMID:3038334
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[[:Category:Kohara_Phage:12A3|12A3]]
[[:Category:Kohara_Phage:4D10|4D10]]
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Kohara Phage
Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=246 Genobase]
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=247 Genobase]
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PMID:3038334  
PMID:3038334  

Revision as of 20:33, 2 September 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ispE

Mnemonic

Isoprenoid

Synonyms

ECK1196, b1208, JW1199, IPK, ychB[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

27.18 minutes 

MG1655: 1262100..1261249
<gbrowseImage> name=NC_000913:1261249..1262100 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1263027..1262176
<gbrowseImage> name=NC_012967:1262176..1263027 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1149970..1150821
<gbrowseImage> name=NC_012759:1149970..1150821 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1264454..1263603
<gbrowseImage> name=AC_000091:1263603..1264454 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1302341..1301490
<gbrowseImage> name=NC_010473:1301490..1302341 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ispET240I

T240I

Loss of activity

seeded from UniProt:P62615

ispEG239R

G239R

Loss of activity

seeded from UniProt:P62615


Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1199

Plasmid clone

Shigen

PMID:16769691[2]

Status:Clone OK

Primer 1:GCCCGGACACAGTGGCCCTCTCC

Primer 2:CCAAGCATGGCTCTGTGCAATGG

12A3

Kohara Phage

Genobase

PMID:3038334[3]

fadR13::Tn10

Linked marker

CAG18497 = CGSC7365[4]

est. P1 cotransduction: 35% [5]

oppC506::Tn10

Linked marker

CAG12169 = CGSC7369[4]

est. P1 cotransduction: 16% [5]
Synonyms:zch-506::Tn10, zci-506::Tn10

4D10

Kohara Phage

Genobase

PMID:3038334[3]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11294

EchoBASE (EcoliWiki Page)

EchoBASE:EB1271

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1208

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C1666

EcoGene (EcoliWiki Page)

EcoGene:EG11294

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:YCHB

ASAP (EcoliWikiPage)

ASAP:ABE-0004056



Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  3. ↑ 3.0 3.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  4. ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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