hyaE:Gene: Difference between revisions

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Fixing Blattner deletion strains in alleles table.
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0003294 hyaE]
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0003294 hyaE::Tn5KAN-2] (FB20078)
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Insertion at nt 126 in Plus orientation
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PMID:12519969
PMID:15262929
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[[asapstrains:ASAP|ASAP]]
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20078]
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At position 126 in Plus orientation, does not contain plasmid  [[pKD46]].<br />ASAP strain name: FB20078
does not contain [[pKD46]]  
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Revision as of 21:44, 9 March 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hyaE

Mnemonic

Hydrogenase 1

Synonyms

ECK0967, b0976, JW0958[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

22.32 minutes 

MG1655: 1035577..1035975
<gbrowseImage> name=NC_000913:1035577..1035975 source=ecoli preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1053445..1053843
<gbrowseImage> name=NC_012967:1053445..1053843 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 938545..938943
<gbrowseImage> name=NC_012759:938545..938943 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1036776..1037174
<gbrowseImage> name=AC_000091:1036776..1037174 source=ecoli_W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1089505..1089903
<gbrowseImage> name=NC_010473:1089505..1089903 source=ecoli_DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔhyaE (Keio:JW0958)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC8954[3]

hyaE::Tn5KAN-2 (FB20078)

Insertion at nt 126 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB20078

does not contain pKD46

ΔhyaE790::kan

PMID:16738554[2]

CGSC:101452



Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0958

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAGCAACGACACGCCATTTGA

Primer 2:CCTGAGGCACGCTCCTGCTGCGG

zcb-3059::Tn10

Linked marker

CAG12094 = CGSC7358[3]

est. P1 cotransduction: 26% [6]

zcc-282::Tn10

Linked marker

CAG18466 = CGSC7359[3]

est. P1 cotransduction: 36% [6]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10472

EchoBASE (EcoliWiki Page)

EchoBASE:EB0467

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0976

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C1117

EcoGene (EcoliWiki Page)

EcoGene:EG10472

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:HYAE

CGSC (EcoliWiki Page)

CGSC:31794

ASAP (EcoliWikiPage)

ASAP:ABE-0003294



Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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