proP:Gene Product(s): Difference between revisions

Standard name	ProP
Synonyms	proline/glycine betaine transporter^[1], B4111^[2]^[1], ProP^[2]^[1]
Product description	ProP proline/betaine MFS transporter^[2]^[3] Proline/betaine permease, minor; osmosensor/osmoregulator^[4]
EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0006810	transport	PMID:6355059^[5]	IGI: Inferred from Genetic Interaction		P	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0015292	uniporter activity	PMID:15205426^[7]	IGI: Inferred from Genetic Interaction		F	Seeded from EcoCyc ^[8]	complete
	GO:0015295	solute:hydrogen symporter activity	PMID:3049595^[9]	IGI: Inferred from Genetic Interaction		F	Seeded from EcoCyc ^[8]	complete
	GO:0042538	hyperosmotic salinity response	PMID:3082857^[10]	IGI: Inferred from Genetic Interaction		P	Seeded from EcoCyc ^[8]	complete
	GO:0016021	integral to membrane	PMID:10026245^[11]	IDA: Inferred from Direct Assay		C		complete
	GO:0016020	membrane	GO_REF:0000002 GO_REF:0000004 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR005829 SP_KW:KW-0397 SP_KW:KW-0472	C	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0006810	transport	GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR004736 InterPro:IPR005828 InterPro:IPR005829 InterPro:IPR007114 SP_KW:KW-0813	P	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0006561	proline biosynthetic process				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0005886	plasma membrane	GO_REF:0000004 GO_REF:0000004 GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997 SP_KW:KW-1003 SP_SL:SL-0037	C	Seeded from EcoCyc ^[8]	complete
	GO:0015293	symporter activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0769	F	Seeded from EcoCyc ^[8]	complete
	GO:0016021	integral to membrane	GO_REF:0000002 GO_REF:0000002 GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR004736 InterPro:IPR005828 InterPro:IPR007114 SP_KW:KW-0812	C	Seeded from EcoCyc 12.5 ^[6]	complete

	GO:0006865	amino acid transport	PMID:10026245^[11]	IDA: Inferred from Direct Assay		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Proline transporter activity is sensitive to the osmolality of the growth medium. Activation and polar localization depend on cardiolipin content of the membrane.

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Source
plasma membrane	C-terminus localized in the cytoplasm with 12 predicted transmembrane domains	Daley et al. (2005) ^[12]
plasma membrane			From EcoCyc^[3]
Pole		Fluorescence microscopy PMID:17504273^[13] PMID:18326496^[14]
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence	at EcoCyc MLKRKKVKPI TLRDVTIIDD GKLRKAITAA SLGNAMEWFD FGVYGFVAYA LGKVFFPGAD PSVQMVAALA TFSVPFLIRP LGGLFFGMLG DKYGRQKILA ITIVIMSIST FCIGLIPSYD TIGIWAPILL LICKMAQGFS VGGEYTGASI FVAEYSPDRK RGFMGSWLDF GSIAGFVLGA GVVVLISTIV GEANFLDWGW RIPFFIALPL GIIGLYLRHA LEETPAFQQH VDKLEQGDRE GLQDGPKVSF KEIATKYWRS LLTCIGLVIA TNVTYYMLLT YMPSYLSHNL HYSEDHGVLI IIAIMIGMLF VQPVMGLLSD RFGRRPFVLL GSVALFVLAI PAFILINSNV IGLIFAGLLM LAVILNCFTG VMASTLPAMF PTHIRYSALA AAFNISVLVA GLTPTLAAWL VESSQNLMMP AYYLMVVAVV GLITGVTMKE TANRPLKGAT PAASDIQEAK EILVEHYDNI EQKIDDIDHE IADLQAKRTR LVQQHPRIDE
Length	500
Mol. Wt	54.848 kDa
pI	7.2 (calculated)
Extinction coefficient	63,830 - 64,330 (calc based on 17 Y, 7 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=proP taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:PROP-MONOMER
UniProt (EcoliWiki Page)	UniProt:P0C0L7
PFAM (EcoliWiki Page)	PFAM:PF00083
RefSeq (EcoliWiki Page)	RefSeq:NP_418535
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ Stalmach, ME et al. (1983) Two proline porters in Escherichia coli K-12. J. Bacteriol. 156 481-6 PubMed
↑ ^6.0 ^6.1 ^6.2 ^6.3 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ Buurman, ET et al. (2004) Multiple paths for nonphysiological transport of K+ in Escherichia coli. J Bacteriol 186 4238-45 PubMed
↑ ^8.0 ^8.1 ^8.2 ^8.3 ^8.4 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
↑ Milner, JL et al. (1988) Proline porter II is activated by a hyperosmotic shift in both whole cells and membrane vesicles of Escherichia coli K12. J Biol Chem 263 14900-5 PubMed
↑ Gowrishankar, J (1986) proP-mediated proline transport also plays a role in Escherichia coli osmoregulation. J Bacteriol 166 331-3 PubMed
↑ ^11.0 ^11.1 Racher, KI et al. (1999) Purification and reconstitution of an osmosensor: transporter ProP of Escherichia coli senses and responds to osmotic shifts. Biochemistry 38 1676-84 PubMed
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
↑ Romantsov, T et al. (2007) Cardiolipin promotes polar localization of osmosensory transporter ProP in Escherichia coli. Mol. Microbiol. 64 1455-65 PubMed
↑ Romantsov, T et al. (2008) Cardiolipin controls the osmotic stress response and the subcellular location of transporter ProP in Escherichia coli. J. Biol. Chem. 283 12314-23 PubMed

proP:Gene Product(s): Difference between revisions

Revision as of 01:40, 16 April 2010

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

References

Categories

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proP:Gene Product(s): Difference between revisions

Revision as of 01:40, 16 April 2010

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

References

Categories

Navigation menu

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