tpr:Gene: Difference between revisions

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!|Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes


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Revision as of 23:32, 10 May 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

tpr

Mnemonic

tyrT region protamine

Synonyms

ECK1224, b1229, JW1219[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

27.72 minutes 

MG1655: 1286411..1286310
gbrowse_img.php?coord=1286310..1286411&genome=ecoli&name=MG1655&trackset=0&width=300&.png

BW2952

NC_012759: 1175031..1175120
gbrowse_img.php?coord=1175031..1175120&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

W3110

 

W3110: 1288765..1288664
gbrowse_img.php?coord=1288664..1288765&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 1326640..1326551
gbrowse_img.php?coord=1326551..1326640&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δtpr (Keio:JW1219)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9105[3]

Δtpr-740::kan

PMID:16738554[2]

CGSC:103194



Notes

The Keio collection[2] lists a deletion of tpr. The insertion in this strain is a duplication of the tpr region.[4]

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1219

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCCGGGGAAGGATGAGAAGCTT

Primer 2:CCGCGCTGCGCGCTTCGCCCTTC

fadR13::Tn10

Linked marker

CAG18497 = CGSC7365[3]

est. P1 cotransduction: 8% [6]

oppC506::Tn10

Linked marker

CAG12169 = CGSC7369[3]

est. P1 cotransduction: 54% [6]
Synonyms:zch-506::Tn10, zci-506::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11016

EchoBASE (EcoliWiki Page)

EchoBASE:EB1009

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1229

EcoGene (EcoliWiki Page)

EcoGene:EG11016

CGSC (EcoliWiki Page)

CGSC:87

ASAP (EcoliWikiPage)

ASAP:ABE-0004131



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Yamamoto, N et al. (2009) Update on the Keio collection of Escherichia coli single-gene deletion mutants. Mol. Syst. Biol. 5 335 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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