dld:Gene: Difference between revisions

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!|Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes


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Revision as of 22:27, 10 May 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dld

Mnemonic

D-Lactate dehydrogenase

Synonyms

ECK2126, b2133, JW2121, ldh[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

47.85 minutes 

MG1655: 2220207..2221922
gbrowse_img.php?coord=2220207..2221922&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2173423..2175138
gbrowse_img.php?coord=2173423..2175138&genome=REL606&name=NC_012967&trackset=0&width=300&.png

BW2952

NC_012759: 2112690..2114405
gbrowse_img.php?coord=2112690..2114405&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

W3110

 

W3110: 2225519..2227234
gbrowse_img.php?coord=2225519..2227234&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 2311215..2312930
gbrowse_img.php?coord=2311215..2312930&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δdld (Keio:JW2121)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9721[3]

dld

PMID:12519969[4]

ASAP

At position 1580 in Plus orientation, contains plasmid pKD46.
ASAP strain name: FB20633

dld

PMID:12519969[4]

ASAP

At position 1580 in Plus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20634

Δdld-759::kan

PMID:16738554[2]

CGSC:100620



Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2121

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCTCTTCCATGACAACAACTGA

Primer 2:CCtTCCACTTCCTGCCAGTTTTT

zbd-3104::Tn10

Linked marker

CAG12021 = CGSC7344[3]

est. P1 cotransduction: 14% [6]
Synonyms:zbc-3105::Tn10, zbd-3105::Tn10

napA722::Tn10

Linked marker

CAG12098 = CGSC7400[3]

est. P1 cotransduction: % [6]
Synonyms:zeg-722, zei-722::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10231

EchoBASE (EcoliWiki Page)

EchoBASE:EB0227

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2133

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2664

EcoGene (EcoliWiki Page)

EcoGene:EG10231

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:DLD

CGSC (EcoliWiki Page)

CGSC:852

ASAP (EcoliWikiPage)

ASAP:ABE-0007048



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 Glasner, JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res. 31 147-51 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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