pheS:Gene: Difference between revisions

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!align=left {{table_heading_style}} |Standard name
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''pheS''  
''pheS''
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!align=left {{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
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Phenylalanine
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!align=left {{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
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''pheS11''
pheSG191D
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G191D
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(decreased affinity for Phe)
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[[cgscmutation:8052|CGSC:8052]]
Strain variation; seeded from [http://www.uniprot.org/uniprot/P08312 UniProt:P08312]  
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''pheS5''(ts)
''pheS11''
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temperature sensitive
 
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PMID:1537809
 
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[[cgscmutation:4877|CGSC:4877]]
[[cgscmutation:8052|CGSC:8052]]
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pheSG191D
''pheS5''(ts)
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G191D
 
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(decreased affinity for Phe)
temperature sensitive
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PMID:1537809
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[[cgscmutation:4877|CGSC:4877]]
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Strain variation; seeded from [http://www.uniprot.org/uniprot/P08312 UniProt:P08312]


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Revision as of 21:34, 25 February 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

pheS

Mnemonic

Phenylalanine

Synonyms

ECK1712, b1714, JW5277, phe-act[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

38.71 minutes 

MG1655: 1796966..1795983
gbrowse_img.php?coord=1795983..1796966&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 1776209..1775226
gbrowse_img.php?coord=1775226..1776209&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 1800656..1799673
gbrowse_img.php?coord=1799673..1800656&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 1887537..1886554
gbrowse_img.php?coord=1886554..1887537&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

pheSG98D

G98D

(in thermosensitive mutant pheS5; might cause subunit disaggregation due to electrostatic repulsion)

Strain variation; seeded from UniProt:P08312

pheSG191D

G191D

(decreased affinity for Phe)

Strain variation; seeded from UniProt:P08312

pheS11

CGSC:8052

pheS5(ts)

temperature sensitive

PMID:1537809[2]

CGSC:4877


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5277

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCTCACATCTCGCAGAACTGGT

Primer 2:CCTTTAAACTGTTTGAGGAAACG

zdi-925::Tn10

Linked marker

CAG12151 = CGSC7385[4]

est. P1 cotransduction: 50% [5]
Synonyms:zdh-925::Tn10

zdj-276::Tn10

Linked marker

CAG18464 = CGSC7387[4]

est. P1 cotransduction: 22% [5]
Synonyms:zdi-276::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10709

EchoBASE (EcoliWiki Page)

EchoBASE:EB0703

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1714

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2112

EcoGene (EcoliWiki Page)

EcoGene:EG10709

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:PHES

CGSC (EcoliWiki Page)

CGSC:400

ASAP (EcoliWikiPage)

ASAP:ABE-0005722


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Kast, P et al. (1992) Identification of the pheS5 mutation, which causes thermosensitivity of Escherichia coli mutant NP37. J. Bacteriol. 174 1686-9 PubMed
  3. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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