murE:Gene: Difference between revisions

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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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murEE344K
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E344K
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(in murE1)
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Strain variation; seeded from [http://www.uniprot.org/uniprot/P22188 UniProt:P22188]
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murEE344K
murEA495S
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E344K
A495S
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Revision as of 17:49, 18 February 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

murE

Mnemonic
Synonyms

ECK0086, b0085, JW0083[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

2.01 minutes 

MG1655: 93166..94653
gbrowse_img.php?coord=93166..94653&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 95970..97457
gbrowse_img.php?coord=95970..97457&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 93166..94653
gbrowse_img.php?coord=93166..94653&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 67270..68757
gbrowse_img.php?coord=67270..68757&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

murEE344K

E344K

(in murE1)

Strain variation; seeded from UniProt:P22188

murE1

CGSC:9851

murEA495S

A495S

(in murE1)

Strain variation; seeded from UniProt:P22188


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0083

Plasmid clone

Shigen

PMID:16769691[2]

Status:Clone OK

Primer 1:GCCGCAGATCGTAATTTGCGCGA

Primer 2:CCTGCAATCACCCCCAGCAGACG

leuO3051::Tn10

Linked marker

CAG12095 = CGSC7324[3]

est. P1 cotransduction: 72% [4]
Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).

zad-220::Tn10

Linked marker

CAG12025 = CGSC7326[3]

est. P1 cotransduction: 7% [4]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10621

EchoBASE (EcoliWiki Page)

EchoBASE:EB0616

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0085

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0103

EcoGene (EcoliWiki Page)

EcoGene:EG10621

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:MURE

CGSC (EcoliWiki Page)

CGSC:475

ASAP (EcoliWikiPage)

ASAP:ABE-0000311



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  3. ↑ 3.0 3.1 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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