frdB:Gene: Difference between revisions

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!align=left {{table_heading_style}} |Standard name
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''frdB''  
''frdB''
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!align=left {{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
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Fumarate reductase
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!align=left {{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
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frdBC63S
frdBC78S
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C63S
C78S
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frdBC66S
frdBC63S
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C66S
C63S
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''ΔfrdB780::kan''
frdBC66S
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C66S
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Affects center 1 (2Fe-2S)
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PMID:16738554
seeded from [http://www.uniprot.org/uniprot/P0AC47 UniProt:P0AC47]  
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[[cgscmutation:100994|CGSC:100994]]
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frdBC78S
''ΔfrdB780::kan''
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C78S
 
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Affects center 1 (2Fe-2S)
 
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PMID:16738554
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[[cgscmutation:100994|CGSC:100994]]
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seeded from [http://www.uniprot.org/uniprot/P0AC47 UniProt:P0AC47]


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Revision as of 21:11, 25 February 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

frdB

Mnemonic

Fumarate reductase

Synonyms

ECK4149, b4153, JW4114[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

94.36 minutes, 94.36 minutes 

MG1655: 4378540..4377806
gbrowse_img.php?coord=4377806..4378540&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 4359228..4358494
gbrowse_img.php?coord=4358494..4359228&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 4385197..4384463
gbrowse_img.php?coord=4384463..4385197&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Strain DH10B

D51N

Nonsynonomous mutation

PMID:18245285[2]

ASAP

This is a SNP in E. coli K-12 Strain DH10B

ΔfrdB (Keio:JW4114)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10963[4]

frdBC58S

C58S

Affects center 1 (2Fe-2S)

seeded from UniProt:P0AC47

frdBC78S

C78S

Affects center 1 (2Fe-2S)

seeded from UniProt:P0AC47

frdBC63S

C63S

Affects center 1 (2Fe-2S)

seeded from UniProt:P0AC47

frdBC66S

C66S

Affects center 1 (2Fe-2S)

seeded from UniProt:P0AC47

ΔfrdB780::kan

PMID:16738554[3]

CGSC:100994



Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4114

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCGCTGAGATGAAAAACCTGAA

Primer 2:CCGCGTGGTTTCAGGGTCGCGAT

cadB2231::Tn10

Linked marker

CAG18488 = CGSC7479[4]

est. P1 cotransduction: 46% [6]
Synonyms:zjd-2231::Tn10

cycA30::Tn10

Linked marker

CAG12073 = CGSC7482[4]

est. P1 cotransduction: 11% [6]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10331

EchoBASE (EcoliWiki Page)

EchoBASE:EB0327

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4153

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C5241

EcoGene (EcoliWiki Page)

EcoGene:EG10331

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:FRDB

CGSC (EcoliWiki Page)

CGSC:741

ASAP (EcoliWikiPage)

ASAP:ABE-0013602



Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Durfee, T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J. Bacteriol. 190 2597-606 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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