rtcB:Gene: Difference between revisions

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Fixing REL gene coord display.
Automated update of mnemonics from EcoGene.
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!align=left {{table_heading_style}} |Standard name
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''rtcB''  
''rtcB''
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RNA terminal phosphate cyclase
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments

Revision as of 21:43, 25 February 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rtcB

Mnemonic

RNA terminal phosphate cyclase

Synonyms

ECK3407, b3421, JW3384, yhgL[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

76.62 minutes, 76.62 minutes 

MG1655: 3556101..3554875
gbrowse_img.php?coord=3554875..3556101&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 3486679..3485453
gbrowse_img.php?coord=3485453..3486679&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 4082337..4083563
gbrowse_img.php?coord=4082337..4083563&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 3653846..3652620
gbrowse_img.php?coord=3652620..3653846&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔrtcB (Keio:JW3384)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10519[3]

rtcB::Tn5KAN

PMID:12519969[4]

ASAP

At position 343 in Plus orientation, contains plasmid pKD46.
ASAP strain name: FB21190

rtcB::Tn5KAN

PMID:12519969[4]

ASAP

At position 343 in Plus orientation, does not contain plasmid pKD46.
ASAP strain name: FB21191

ΔrtcB754::kan

PMID:16738554[2]

CGSC:102844



Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3384

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAATTACGAATTACTGACCAC

Primer 2:CCTCCTTTTACGCACACCACCTG

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[3]

est. P1 cotransduction: 9% [6]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

glgP721::Tn10

Linked marker

CAG18638 = CGSC7451[3]

est. P1 cotransduction: 75% [6]
Synonyms:zhg-3086::Tn10, zhh-21::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:G7751

EchoBASE (EcoliWiki Page)

EchoBASE:EB2774

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3421

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4198

EcoGene (EcoliWiki Page)

EcoGene:EG12939

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:RTCB

ASAP (EcoliWikiPage)

ASAP:ABE-0011170



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 Glasner, JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res. 31 147-51 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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