DIGE Training Manual: Difference between revisions

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[[Category:Education]]
[[Category:Education]]
[[Category:EcoliWiki for Education ]]
[[Category:EcoliWiki for Education ]]
[[Category:Methods]]
[[Category:Methods|Dige Training Manual]]
[[Category:Gel electrophoresis methods]]
[[Category:Gel electrophoresis methods]]
This manual was compiled by Dr. L. Dangott, [http://www.tamupcl.com Protein Chemistry Laboratory] Texas A&M University.
This manual was compiled by Dr. L. Dangott, [http://www.tamupcl.com Protein Chemistry Laboratory] Texas A&M University.


[http://ecoliwiki.net/colipedia/index.php/DIGE_Training_Workshop_January_2009_at_Texas_A%26M DIGE Training Workshop January 2009]
[[DIGE Training Workshop January 2009 at Texas A&M]]


Click below on a link to a chapter or section.
Click below on a link to a chapter or section.
==DIGE Training Manual==
==DIGE Training Manual==
[[Image:Chapter_1.pdf]] - Protein Extraction and Estimation
[[Media:Chapter_1.pdf]] - Protein Extraction and Estimation


[[Image:Chapter_2.pdf]] - Protein Concentrating Techniques
[[Media:Chapter_2.pdf]] - Protein Concentrating Techniques


[[Image:Chapter_3.pdf]] - DIGE Labeling
[[Media:Chapter_3.pdf]] - DIGE Labeling


[[Image:Chapter_4.pdf]] - IEF Gels
[[Media:Chapter_4.pdf]] - IEF Gels


[[Image:Chapter_5.doc]] - SDS-PAGE & 2 Dimensional Gels
[[Media:DIGE Chapter 5.pdf]] - SDS-PAGE & 2 Dimensional Gels


[[Image:Chapter_5_2DGels.pdf]] - 2 Dimensional Gels
[[Media:Chapter_5_2DGels.pdf]] - 2 Dimensional Gels


[[Image:Recipe_for_Chapter_5.doc]] - Recipe for SDS-PAGE Gel
[[Media:DIGE Recipe for Chapter 5.pdf]] - Recipe for SDS-PAGE Gel


[[Image:Chapter6Part1.pdf]] - Typhoon Trio Imaging (Part 1)
[[Media:Chapter6Part1.pdf]] - Typhoon Trio Imaging (Part 1)


[[Image:Chapter6Part2.doc]] - Typhoon Trio Imaging (Part 2)
[[Image:Chapter6Part2.doc]] - Typhoon Trio Imaging (Part 2)


[[Image:Chapter6Part3.pdf]] - Typhoon Trio Imaging (Part 3)
[[Media:Chapter6Part3.pdf]] - Typhoon Trio Imaging (Part 3)


[[Image:Chapter7Part1.pdf]] - Computer Analysis of DIGE Gels (Part 1)
[[Media:Chapter7Part1.pdf]] - Computer Analysis of DIGE Gels (Part 1)


[[Image:Chapter7Part2.pdf]] - Computer Analysis of DIGE Gels (Part 2)
[[Media:Chapter7Part2.pdf]] - Computer Analysis of DIGE Gels (Part 2)


[[Image:Chapter7Part3.pdf]] - Computer Analysis of DIGE Gels (Part 3)
[[Media:Chapter7Part3.pdf]] - Computer Analysis of DIGE Gels (Part 3)


[[Image:Chapter7Part4.pdf]] - Computer Analysis of DIGE Gels (Part 4)
[[Media:Chapter7Part4.pdf]] - Computer Analysis of DIGE Gels (Part 4)


[[Image:Chapter_8_Staining.pdf]] - Fluorescent Protein Detection
[[Media:Chapter_8_Staining.pdf]] - Fluorescent Protein Detection

Latest revision as of 17:54, 28 October 2010

This manual was compiled by Dr. L. Dangott, Protein Chemistry Laboratory Texas A&M University.

DIGE Training Workshop January 2009 at Texas A&M

Click below on a link to a chapter or section.

DIGE Training Manual

Media:Chapter_1.pdf - Protein Extraction and Estimation

Media:Chapter_2.pdf - Protein Concentrating Techniques

Media:Chapter_3.pdf - DIGE Labeling

Media:Chapter_4.pdf - IEF Gels

Media:DIGE Chapter 5.pdf - SDS-PAGE & 2 Dimensional Gels

Media:Chapter_5_2DGels.pdf - 2 Dimensional Gels

Media:DIGE Recipe for Chapter 5.pdf - Recipe for SDS-PAGE Gel

Media:Chapter6Part1.pdf - Typhoon Trio Imaging (Part 1)

File:Chapter6Part2.doc - Typhoon Trio Imaging (Part 2)

Media:Chapter6Part3.pdf - Typhoon Trio Imaging (Part 3)

Media:Chapter7Part1.pdf - Computer Analysis of DIGE Gels (Part 1)

Media:Chapter7Part2.pdf - Computer Analysis of DIGE Gels (Part 2)

Media:Chapter7Part3.pdf - Computer Analysis of DIGE Gels (Part 3)

Media:Chapter7Part4.pdf - Computer Analysis of DIGE Gels (Part 4)

Media:Chapter_8_Staining.pdf - Fluorescent Protein Detection