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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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{|   id="U46a9c1e9706c6" class=" tableEdit Gene_nomenclature_table"


|-
|-
!align=left {{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Standard name
||
||
''ispE''  
''ispE''
|-
|-
!align=left {{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
||
||
 
Isoprenoid
|-
|-
!align=left {{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
||
||
''ECK1196, b1208, JW1199, IPK, ychB''<ref name='LIB:Riley_2006'/>
''ECK1196, b1208, JW1199, IPK, ychB''<ref name='LIB:Riley_2006'/>, ipk, ychB''


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*  
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****************************************************************************************** -->{{Gene_location_help}}
****************************************************************************************** -->{{Gene_location_help}}
{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="W46a9c1e9f0c0c"  class=" tableEdit Gene_location_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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27.18 minutes&nbsp;
27.18 minutes&nbsp;
|
|
MG1655: 1262100..1261249<br />{{GBrowseFigureByCoord|1260249|1263100|ecoli|MG1655}}
MG1655: 1262100..1261249<br /><gbrowseImage>
name=NC_000913:1261249..1262100
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11294 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11294 EcoCyc]
{{GBrowseLink|ecoli|NC_000913|1260249|1263100|GBrowse(MG1655)}}
{{GBrowseLink|MG1655|NC_000913|1260249|1263100|GBrowse(MG1655)}}
   
   
|
|
{{GBrowseFastaLink|ecoli|NC_000913|1261249|1262100|FASTA Dump}}
{{GBrowseFastaLink|MG1655|NC_000913|1261249|1262100|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11294 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG11294 EcoCyc Sequence Dump]  
|-
|
[[REL606]]
|
|
NC_012967: 1263027..1262176<br /><gbrowseImage>
name=NC_012967:1262176..1263027
source=REL606
preset=GeneLocation
</gbrowseImage>
|
{{GBrowseLink|REL606|NC_012967|1262176|1263027|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|1262176|1263027|FASTA Dump}}
|-
|
[[BW2952]]
|
|
NC_012759: 1149970..1150821<br /><gbrowseImage>
name=NC_012759:1149970..1150821
source=BW2952
preset=GeneLocation
</gbrowseImage>
|
{{GBrowseLink|BW2952|NC_012759|1149970|1150821|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|1149970|1150821|FASTA Dump}}
|-  
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&nbsp;
&nbsp;
|
|
W3110: 1264454..1263603<br />{{GBrowseFigureByCoord|1262603|1265454|ecoli_W3110|W3110}}  
W3110: 1264454..1263603<br /><gbrowseImage>
name=NC_007779:1263603..1264454
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|W3110|NC_007779|1262603|1265454|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|NC_007779|1263603|1264454|FASTA Dump}}  
|-
|
[[DH10B]]
|
 
|
DH10B: 1302341..1301490<br /><gbrowseImage>
name=NC_010473:1301490..1302341
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
|
{{GBrowseLink|ecoli_W3110|AC_000091|1262603|1265454|GBrowse(W3110)}}  
{{GBrowseLink|DH10B|NC_010473|1299788|1304043|GBrowse(DH10B)}}
|
|
{{GBrowseFastaLink|ecoli_W3110|AC_000091|1263603|1264454|FASTA Dump}}  
{{GBrowseFastaLink|DH10B|NC_010473|1299788|1304043|FASTA Dump}}  


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{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="I46a9c1ea9e002"  class=" tableEdit Gene_sequence_features_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes


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*  
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****************************************************************************************** -->{{Gene_alleles_help}}
****************************************************************************************** -->{{Gene_alleles_help}}
{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" {{Prettytable}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="C46a9c1eae01cf"  class=" tableEdit Gene_allele_table dataTable" 
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|
ispET240I
|
|
T240I
|
|
Loss of activity
|
|
|
seeded from [http://www.uniprot.org/uniprot/P62615 UniProt:P62615]
|-
|
ispEG239R
|
|
G239R
|
|
Loss of activity
|


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|
seeded from [http://www.uniprot.org/uniprot/P62615 UniProt:P62615]
 
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===Notes===
===Notes===


==Genetic Interactions==
{{BacktoTop}}
<protect><!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.12969.P4e04be0147cd1-->
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|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
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<!-- Genetic Interacdtions Notes -->
===Notes===


==Genetic Resources==
==Genetic Resources==
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!|Resource!!Resource Type!!Source!!Notes/Reference
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Primer 2:CCAAGCATGGCTCTGTGCAATGG  
Primer 2:CCAAGCATGGCTCTGTGCAATGG  
|-
|
[[:Category:Kohara_Phage:12A3|12A3]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=246 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:4D10|4D10]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=247 Genobase]
|
PMID:3038334
|-  
|-  
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Synonyms:zch-506::Tn10, zci-506::Tn10  
Synonyms:zch-506::Tn10, zci-506::Tn10  


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|- {{table_heading_style}}
|- {{table_heading_style}}
!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11294 EcoCyc:EG11294]
|
|-
|
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
|
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB1271 EchoBASE:EB1271]
EcoCyc:EG11294
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
[[EcoGene]]
|
|
[http://chase.ou.edu/macro/searchlink.php?bnum=b1208 EcoliGenExpDB:b1208]
EcoGene:EG11294
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://BioCyc.org/ECOL199310/new-image?object=C1666 ECOL199310CYC:C1666]
RegulonDB:ECK120001267
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
|
[http://ecogene.org/geneinfo.php?eg_id=EG11294 EcoGene:EG11294]
[http://www.ncbi.nlm.nih.gov/gene?term=945774 GeneID:945774]
|
|
Escherichia coli str. K-12 substr. MG1655
|-  
|-  
|
|
[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://BioCyc.org/ECOO157/new-image?object=YCHB ECOO157CYC:YCHB]
EchoBASE:EB1271
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
[[ASAP]]
|
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0004056&formSubmitReturn=1 ASAP:ABE-0004056]
ASAP:ABE-0004056
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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==References==
==References==
{{BacktoTop}}
{{BacktoTop}}
 
{{RefHelp}}
<references/>
<references />
__NOTOC__
__NOTOC__
</protect></noinclude>
===Categories===
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:MG1655]]
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[[Category:Genes not in the Keio knockout collection]]
[[Category:Genes not in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:isp genes]][[Category:Gene_List:DH10B]]
[[Category:isp genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

Latest revision as of 20:30, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ispE

Mnemonic

Isoprenoid

Synonyms

ECK1196, b1208, JW1199, IPK, ychB[1], ipk, ychB

edit table


Notes

Location(s) and DNA Sequence

[back to top]


See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

27.18 minutes 

MG1655: 1262100..1261249
<gbrowseImage> name=NC_000913:1261249..1262100 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1263027..1262176
<gbrowseImage> name=NC_012967:1262176..1263027 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1149970..1150821
<gbrowseImage> name=NC_012759:1149970..1150821 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1264454..1263603
<gbrowseImage> name=NC_007779:1263603..1264454 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1302341..1301490
<gbrowseImage> name=NC_010473:1301490..1302341 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ispET240I

T240I

Loss of activity

seeded from UniProt:P62615

ispEG239R

G239R

Loss of activity

seeded from UniProt:P62615

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Notes

Genetic Interactions

[back to top]



Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
edit table


Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1199

Plasmid clone

Shigen

PMID:16769691[2]

Status:Clone OK

Primer 1:GCCCGGACACAGTGGCCCTCTCC

Primer 2:CCAAGCATGGCTCTGTGCAATGG

12A3

Kohara Phage

Genobase

PMID:3038334[3]

4D10

Kohara Phage

Genobase

PMID:3038334[3]

fadR13::Tn10

Linked marker

CAG18497 = CGSC7365[4]

est. P1 cotransduction: 35% [5]

oppC506::Tn10

Linked marker

CAG12169 = CGSC7369[4]

est. P1 cotransduction: 16% [5]
Synonyms:zch-506::Tn10, zci-506::Tn10

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Notes

Accessions in Other Databases

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Database Accession Notes

EcoCyc

EcoCyc:EG11294

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11294

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001267

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945774

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1271

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004056

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  3. ↑ 3.0 3.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  4. ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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