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W3110: 3589715..3586929<br /><gbrowseImage>
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{{GBrowseLink|W3110|AC_000091|3585929|3590715|GBrowse(W3110)}}  
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="S46aa00d8b0b70"  class=" tableEdit Gene_allele_table dataTable"   
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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&Delta;polA (Keio:JW3835)
''polA(del)'' (Keio:JW3835)
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deletion
deletion
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PMID:16738554
PMID:16738554
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=109051 CGSC10770]<ref name='LIB:cgsc'/>  
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br />[http://cgsc2.biology.yale.edu/Strain.php?ID=109051 CGSC10770]<ref name='LIB:cgsc'/>  
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''&#916;polA726::kan''
''polA726(del)''::kan
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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10746 ECOCYC:EG10746]
EcoCyc:EG10746
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[EcoGene]]
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EcoGene:EG10746
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[RegulonDB]]
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[http://ecogene.org/geneinfo.php?eg_id=EG10746 EcoGene:EG10746]
RegulonDB:ECK120000737
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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Escherichia coli str. K-12 substr. MG1655  
Escherichia coli str. K-12 substr. MG1655  
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[[EchoBASE]]
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EchoBASE:EB0739
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:ABE-0012618
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Latest revision as of 20:50, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

polA

Mnemonic

Polymerase

Synonyms

ECK3855, b3863, JW3835, resA[1], resA

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

87.18 minutes 

MG1655: 4044989..4047775
<gbrowseImage> name=NC_000913:4044989..4047775 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4025263..4028049
<gbrowseImage> name=NC_012967:4025263..4028049 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3934658..3937444
<gbrowseImage> name=NC_012759:3934658..3937444 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3589715..3586929
<gbrowseImage> name=NC_007779:3586929..3589715 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4143909..4146695
<gbrowseImage> name=NC_010473:4143909..4146695 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

polA(del) (Keio:JW3835)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10770[3]

polA12(ts)

temperature sensitive

PMID:776979[4] PMID:765763[5]

CGSC:5215

polA1(Am)

PMID:765763[5] PMID:4902143[6]

CGSC:6245

amber (UAG) mutation

polA480(ts, EX)

"temperature sensitive, exonuclease activity mutation "

PMID:776979[4] PMID:765763[5]

CGSC:6383

polA546(ts, EX)

"temperature sensitive, exonuclease activity mutation "

CGSC:6418

polA107

PMID:765763[5]

CGSC:8417

polA11

CGSC:8660

polA4113(ts)

temperature sensitive

PMID:765763[5]

CGSC:10274

polA34(ts)

temperature sensitive

CGSC:10522

polA0

CGSC:10858

polA591

CGSC:11054

polA20

CGSC:11306

polA5

PMID:359556[7] PMID:765763[5]

CGSC:16005

polA6

PMID:12497[8]

CGSC:19630

polA726(del)::kan

PMID:16738554[2]

CGSC:102396


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Notes

The Keio collection[2] lists a deletion of polA. The insertion in this strain is a duplication of the polA region.[9]


Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3835

Plasmid clone

Shigen

PMID:16769691[10]

Status:Clone OK

Primer 1:GCCGTTCAGATCCCCCAAAATCC

Primer 2:CCGTGCGCCTGATCCCAGTTTTC

8D12

Kohara Phage

Genobase

PMID:3038334[11]

10H11

Kohara Phage

Genobase

PMID:3038334[11]

fad-751::Tn10

Linked marker

CAG18496 = CGSC7465[3]

est. P1 cotransduction: 53% [12]
Synonyms:fadAB101::Tn10

zih-35::Tn10

Linked marker

CAG18495 = CGSC7467[3]

est. P1 cotransduction: 50% [12]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10746

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10746

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000737

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948356

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0739

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012618

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 Uyemura, D et al. (1976) Biochemical characterization of mutant forms of DNA polymerase I from Escherichia coli. II. The polAex1 mutation. J. Biol. Chem. 251 4085-9 PubMed
  5. ↑ 5.0 5.1 5.2 5.3 5.4 5.5 Grindley, ND & Kelley, WS (1976) Effects of different alleles of the E. coli K12 pol A gene on the replication of non-transferring plasmids. Mol. Gen. Genet. 143 311-8 PubMed
  6. ↑ Gross, J & Gross, M (1969) Genetic analysis of an E. coli strain with a mutation affecting DNA polymerase. Nature 224 1166-8 PubMed
  7. ↑ Matson, SW et al. (1978) On the processive mechanism of Escherichia coli DNA Polymerase I. The polA5 mutation. J. Biol. Chem. 253 7851-6 PubMed
  8. ↑ Kelly, WS & Grindley, ND (1976) polA6, A mutation affecting the DNA binding capacity of DNA polymerase I. Nucleic Acids Res. 3 2971-84 PubMed
  9. ↑ Yamamoto, N et al. (2009) Update on the Keio collection of Escherichia coli single-gene deletion mutants. Mol. Syst. Biol. 5 335 PubMed
  10. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  11. ↑ 11.0 11.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  12. ↑ 12.0 12.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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