dcd:Gene: Difference between revisions

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!align=left {{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
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''ECK2059, b2065, JW2050, paxA, dus''<ref name='LIB:Riley_2006'/>
''ECK2059, b2065, JW2050, paxA, dus''<ref name='LIB:Riley_2006'/>, dus''


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****************************************************************************************** -->{{Gene_location_help}}
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{|   id="F46a9d53e0d2de"  class=" tableEdit Gene_location_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="F46a9d53e0d2de"  class=" tableEdit Gene_location_table"   
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!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11418 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11418 EcoCyc]
{{GBrowseLink|ecoli|NC_000913|2138658|2141239|GBrowse(MG1655)}}
{{GBrowseLink|MG1655|NC_000913|2138658|2141239|GBrowse(MG1655)}}
   
   
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{{GBrowseFastaLink|ecoli|NC_000913|2139658|2140239|FASTA Dump}}
{{GBrowseFastaLink|MG1655|NC_000913|2139658|2140239|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11418 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG11418 EcoCyc Sequence Dump]  
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{{GBrowseLink|BW2952|012759|2032141|NC_2032722|GBrowse (BW2952)}}
{{GBrowseLink|BW2952|NC_012759|2032141|2032722|GBrowse (BW2952)}}
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{{GBrowseFastaLink|BW2952|012759|2032141|NC_2032722|FASTA Dump}}  
{{GBrowseFastaLink|BW2952|NC_012759|2032141|2032722|FASTA Dump}}  
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W3110: 2144352..2143771<br /><gbrowseImage>
W3110: 2144352..2143771<br /><gbrowseImage>
name=AC_000091:2143771..2144352
name=NC_007779:2143771..2144352
source=MG1655_W3110
source=W3110
preset=GeneLocation
preset=GeneLocation
</gbrowseImage>
</gbrowseImage>


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{{GBrowseLink|ecoli_W3110|AC_000091|2142771|2145352|GBrowse(W3110)}}  
{{GBrowseLink|W3110|NC_007779|2142771|2145352|GBrowse(W3110)}}  
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{{GBrowseFastaLink|ecoli_W3110|AC_000091|2143771|2144352|FASTA Dump}}  
{{GBrowseFastaLink|W3110|NC_007779|2143771|2144352|FASTA Dump}}  
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{{GBrowseLink|ecoli_DH10B|NC_010473|2229504|2232409|GBrowse(DH10B)}}
{{GBrowseLink|DH10B|NC_010473|2229504|2232409|GBrowse(DH10B)}}
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{{GBrowseFastaLink|ecoli_DH10B|NC_010473|2229504|2232409|FASTA Dump}}  
{{GBrowseFastaLink|DH10B|NC_010473|2229504|2232409|FASTA Dump}}  


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****************************************************************************************** -->{{Gene_sequence_features_help}}
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{|   id="V46a9d53eb6066"  class=" tableEdit Gene_sequence_features_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="V46a9d53eb6066"  class=" tableEdit Gene_sequence_features_table"   
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!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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{| {{Prettytable}} id="D46a9d53f069f8"  class=" tableEdit Gene_allele_table dataTable"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="D46a9d53f069f8"  class=" tableEdit Gene_allele_table dataTable"   
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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{|   id="K4e04b992e61b1"  class=" tableEdit Genetic_interaction_table dataTable"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="K4e04b992e61b1"  class=" tableEdit Genetic_interaction_table dataTable"   
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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
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{|   id="J46a9d53fafc02"  class=" tableEdit Gene_resource_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="J46a9d53fafc02"  class=" tableEdit Gene_resource_table"   
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!|Resource!!Resource Type!!Source!!Notes/Reference
!|Resource!!Resource Type!!Source!!Notes/Reference
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Primer 2:CCGTCTTTATCGATTCGGCTGGC  
Primer 2:CCGTCTTTATCGATTCGGCTGGC  
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[[:Category:Kohara_Phage:2E1|2E1]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=355 Genobase]
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PMID:3038334
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[[:Category:Kohara_Phage:3G4|3G4]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=356 Genobase]
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PMID:3038334
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****************************************************************************************** -->{{Gene_accessions_help}}
{|   id="G46a9d5404e1ed"  class=" tableEdit Gene_accessions_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="G46a9d5404e1ed"  class=" tableEdit Gene_accessions_table"   
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|- {{table_heading_style}}
!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11418 EcoCyc:EG11418]
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB1389 EchoBASE:EB1389]
EcoCyc:EG11418
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
[[EcoGene]]
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[http://chase.ou.edu/macro/searchlink.php?bnum=b2065 EcoliGenExpDB:b2065]
EcoGene:EG11418
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
[[RegulonDB]]
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[http://BioCyc.org/ECOL199310/new-image?object=C2592 ECOL199310CYC:C2592]
RegulonDB:ECK120001383
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG11418 EcoGene:EG11418]
[http://www.ncbi.nlm.nih.gov/gene?term=946593 GeneID:946593]
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Escherichia coli str. K-12 substr. MG1655
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
[[EchoBASE]]
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[http://BioCyc.org/ECOO157/new-image?object=DCD ECOO157CYC:DCD]
EchoBASE:EB1389
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
[[ASAP]]
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[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/875 CGSC:875]
ASAP:ABE-0006835
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0006835&formSubmitReturn=1 ASAP:ABE-0006835]
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{|   id="P46a6f2838a43c"  class=" tableEdit Links_table"   
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!|Name!!URL!!Comments
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Latest revision as of 20:40, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dcd

Mnemonic

dCTP deaminase

Synonyms

ECK2059, b2065, JW2050, paxA, dus[1], dus


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

46.12 minutes 

MG1655: 2140239..2139658
<gbrowseImage> name=NC_000913:2139658..2140239 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2075421..2074840
<gbrowseImage> name=NC_012967:2074840..2075421 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2032141..2032722
<gbrowseImage> name=NC_012759:2032141..2032722 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2144352..2143771
<gbrowseImage> name=NC_007779:2143771..2144352 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2231247..2230666
<gbrowseImage> name=NC_010473:2230666..2231247 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2139658

Edman degradation

PMID:1324907[2]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δdcd (Keio:JW2050)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC9684[4]

dcd-1

CGSC:9217

Δdcd-763::kan

PMID:16738554[3]

CGSC:100534



Notes

Genetic Interactions

[back to top]



Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2050

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCCGTCTGTGTGACCGAGATAT

Primer 2:CCGTCTTTATCGATTCGGCTGGC

2E1

Kohara Phage

Genobase

PMID:3038334[6]

3G4

Kohara Phage

Genobase

PMID:3038334[6]

zef-3129::Tn10

Linked marker

CAG12099 = CGSC7397[4]

est. P1 cotransduction: 6% [7]
Synonyms:zee-3129::Tn10

zbd-3104::Tn10

Linked marker

CAG12021 = CGSC7344[4]

est. P1 cotransduction: 23% [7]
Synonyms:zbc-3105::Tn10, zbd-3105::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11418

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11418

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001383

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946593

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1389

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0006835

Escherichia coli str. K-12 substr. MG1655


Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Wang, L & Weiss, B (1992) dcd (dCTP deaminase) gene of Escherichia coli: mapping, cloning, sequencing, and identification as a locus of suppressors of lethal dut (dUTPase) mutations. J. Bacteriol. 174 5647-53 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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