rpoA:Gene Product(s): Difference between revisions
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!align=left {{table heading style}} |Synonyms | !align=left {{table heading style}} |Synonyms | ||
|| | || | ||
RNA polymerase, alpha subunit<ref name='LIB:Riley_2006'/>, B3295<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Sez<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Phs<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Pez<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, RpoA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> | ''RNA polymerase, alpha subunit<ref name='LIB:Riley_2006'/>, B3295<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Sez<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Phs<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Pez<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, RpoA<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , ECK3282, JW3257, pez, phs, sez, b3295'' | ||
|- | |- | ||
!align=left {{table heading style}} |Product description | !align=left {{table heading style}} |Product description | ||
| Line 74: | Line 74: | ||
****************************************************************************************** | ****************************************************************************************** | ||
* | * | ||
** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | |||
* | * | ||
****************************************************************************************** -->{{Product_GO_help}} | ****************************************************************************************** -->{{Product_GO_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="H46aade42ee89a" class=" tableEdit GO_table_product dataTable" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Qualifier!!GO ID!!GO term name!!Reference | !|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status | ||
|- | |- | ||
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Seeded from [[EcoCyc]] (v14.0) | Seeded from [[EcoCyc]] (v14.0) | ||
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complete | |||
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| | | | ||
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Seeded from [[EcoCyc]] (v14.0) | Seeded from [[EcoCyc]] (v14.0) | ||
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complete | |||
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| | | | ||
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Seeded from [[EcoCyc]] (v14.0) | Seeded from [[EcoCyc]] (v14.0) | ||
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complete | |||
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Seeded from [[EcoCyc]] (v14.0) | Seeded from [[EcoCyc]] (v14.0) | ||
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complete | |||
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Seeded from [[EcoCyc]] (v14.0) | Seeded from [[EcoCyc]] (v14.0) | ||
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complete | |||
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| | | | ||
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Seeded from [[EcoCyc]] (v14.0) | Seeded from [[EcoCyc]] (v14.0) | ||
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complete | |||
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| | | | ||
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Seeded from [[EcoCyc]] (v14.0) | Seeded from [[EcoCyc]] (v14.0) | ||
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complete | |||
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Seeded from [[EcoCyc]] (v14.0) | Seeded from [[EcoCyc]] (v14.0) | ||
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complete | |||
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Seeded from [[EcoCyc]] (v14.0) | Seeded from [[EcoCyc]] (v14.0) | ||
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complete | |||
|- | |- | ||
| | | | ||
Deprecated | |||
| | | | ||
GO:0008270 | GO:0008270 | ||
| Line 267: | Line 267: | ||
F | F | ||
| | | | ||
The PMID listed is for a paper on spinal cord injury. I cannot find any experimental evidence for Zn binding by the alpha subunit of RNA polymerase. [[User:Siegele|Siegele]] ([[User talk:Siegele|talk]]) 16:16, 15 May 2019 (CDT) | |||
| | | | ||
complete | complete | ||
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complete | |||
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complete | |||
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complete | |||
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complete | |||
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complete | |||
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complete | |||
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complete | |||
|- class="tableEdit_footer" | |- class="tableEdit_footer" | ||
|< | |<div class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.27049.H46aade42ee89a&page=27049&pagename={{FULLPAGENAMEE}}&type=&template=GO_table_product edit table]</div> || || || || || || || || | ||
|} | |} | ||
<!--box uid=421aa90e079fa326b6494f812ad13e79.27049.H46aade42ee89a--> | <!--box uid=421aa90e079fa326b6494f812ad13e79.27049.H46aade42ee89a--> | ||
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* | * | ||
****************************************************************************************** -->{{Product_interactions_help}} | ****************************************************************************************** -->{{Product_interactions_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="I46aade4f0f900" class=" tableEdit Product_interactions_table dataTable" | ||
|- {{table heading style}} | |- {{table heading style}} | ||
!|Partner Type!!Partner!!Notes!!References!!Evidence | !|Partner Type!!Partner!!Notes!!References!!Evidence | ||
| Line 1,208: | Line 1,208: | ||
Protein | Protein | ||
| | | | ||
mreB | |||
| | | | ||
| | | | ||
PMID:19402753 | |||
| | | | ||
MALDI(Z-score):36.959637 | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
rpsP | |||
| | | | ||
| | | | ||
PMID:19402753 | |||
| | | | ||
LCMS(ID Probability):99.6 | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
rpsS | |||
| | | | ||
| | | | ||
PMID:19402753 | |||
| | | | ||
LCMS(ID Probability):99.6 | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
rplU | |||
| | | | ||
| | | | ||
PMID:19402753 | |||
| | | | ||
LCMS(ID Probability):99.6 | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
rpsT | |||
| | | | ||
| | | | ||
PMID:19402753 | |||
| | | | ||
LCMS(ID Probability):99.6 | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
rpsN | |||
| | | | ||
| | | | ||
PMID:19402753 | |||
| | | | ||
LCMS(ID Probability):99.6 | |||
|- | |- | ||
| | | | ||
Protein | Protein | ||
| | | | ||
rplP | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 MALDI(Z-score):3.353874 | |||
|- | |||
| | |||
Protein | |||
| | | | ||
rplX | |||
| | | | ||
| | | | ||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 | |||
|- | |||
| | |||
Protein | |||
| | |||
rpmG | |||
| | |||
| | | | ||
| | PMID:19402753 | ||
| | | | ||
LCMS(ID Probability):99.6 | |||
|- | |||
| | |||
Protein | |||
| | |||
rpmA | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 | |||
|- | |||
| | |||
Protein | |||
| | |||
rpsA | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
MALDI(Z-score):21.278856 | |||
|- | |||
| | |||
Protein | |||
| | |||
rpmB | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 | |||
|- | |||
| | |||
Protein | |||
| | |||
rplA | |||
| | | | ||
| | | | ||
| | PMID:19402753 | ||
| | | | ||
MALDI(Z-score):32.556213 | |||
|- | |||
| | |||
Protein | |||
| | |||
rplS | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 | |||
|- | |||
| | |||
Protein | |||
| | |||
aspS | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 MALDI(Z-score):8.467531 | |||
|- | |||
| | |||
Protein | |||
| | |||
rpsJ | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):97.9 MALDI(Z-score):16.475126 | |||
|- | |||
| | |||
Protein | |||
| | |||
rpsF | |||
| | |||
| | | | ||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 | |||
|- | |||
| | |||
Protein | |||
| | |||
rplW | |||
| | |||
|- | |||
| | |||
| | |||
| | | | ||
| | PMID:19402753 | ||
| | | | ||
LCMS(ID Probability):99.6 | |||
|- | |||
| | |||
Protein | |||
| | |||
rplM | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 MALDI(Z-score):17.053160 | |||
|- | |||
| | |||
Protein | |||
| | |||
hupA | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 MALDI(Z-score):9.189812 | |||
|- | |||
| | |||
Protein | |||
| | |||
rpsC | |||
| | |||
| | | | ||
PMID:19402753 | |||
| | | | ||
MALDI(Z-score):41.404786 | |||
|- | |||
| | |||
Protein | |||
| | |||
rplB | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
MALDI(Z-score):37.610726 | |||
|- | |||
| | |||
Protein | |||
| | |||
rpsO | |||
| | |||
| | | | ||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 | |||
|- | |||
| | |||
Protein | |||
| | | | ||
rpsD | |||
| | |||
|- | |||
| | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
MALDI(Z-score):34.513588 | |||
|- | |||
| | |||
Protein | |||
| | |||
rho | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
MALDI(Z-score):37.049801 | |||
|- | |||
| | |||
Protein | |||
| | |||
cspC | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 MALDI(Z-score):13.185220 | |||
|- | |||
| | |||
Protein | |||
| | |||
rplD | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 MALDI(Z-score):27.436806 | |||
|- | |||
| | |||
Protein | |||
| | |||
greA | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):93.1 MALDI(Z-score):17.053160 | |||
|- | |||
| | |||
Protein | |||
| | |||
rpsU | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 | |||
|- | |||
| | |||
Protein | |||
| | |||
pssA | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
MALDI(Z-score):19.670165 | |||
|- | |||
| | |||
Protein | |||
| | |||
nusG | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 MALDI(Z-score):35.117471 | |||
|- | |||
| | |||
Protein | |||
| | |||
rpoB | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 MALDI(Z-score):39.950272 | |||
|- | |||
| | |||
Protein | |||
| | |||
rpoC | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 MALDI(Z-score):39.702103 | |||
|- | |||
| | |||
Protein | |||
| | |||
rapA | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 MALDI(Z-score):18.240902 | |||
|- | |||
| | |||
Protein | |||
| | |||
rpoZ | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 MALDI(Z-score):38.277175 | |||
|- | |||
| | |||
Protein | |||
| | |||
rffM | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 | |||
|- | |||
| | |||
Protein | |||
| | |||
rpoD | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 MALDI(Z-score):40.069162 | |||
|- | |||
| | |||
Protein | |||
| | |||
rplL | |||
| | |||
| | |||
PMID:19402753 | |||
| | |||
LCMS(ID Probability):99.6 | |||
|- | |||
| | |||
Protein | |||
| | |||
Subunits of [[:Category:Complex:RNA polymerase sigma 24|RNA polymerase sigma 24]] | |||
| | |||
could be indirect | |||
| | |||
| | |||
|- | |||
| | |||
Protein | |||
| | |||
Subunits of [[:Category:Complex:RNA polymerase sigma 38|RNA polymerase sigma 38]] | |||
| | |||
could be indirect | |||
| | |||
| | |||
|- | |||
| | |||
Protein | |||
| | |||
Subunits of [[:Category:Complex:RNA polymerase sigma32|RNA polymerase sigma32]] | |||
| | |||
could be indirect | |||
| | |||
| | |||
|- | |||
| | |||
Protein | |||
| | |||
Subunits of [[:Category:Complex:RNA polymerase sigma54|RNA polymerase sigma54]] | |||
| | |||
could be indirect | |||
| | |||
== | | | ||
{{ | |||
<!--box uid=421aa90e079fa326b6494f812ad13e79.27049. | |- | ||
<!-- | | | ||
****************************************************************************************** | Protein | ||
* | | | ||
Subunits of [[:Category:Complex:RNA polymerase sigma70|RNA polymerase sigma70]] | |||
| | |||
could be indirect | |||
| | |||
| | |||
|- | |||
| | |||
Protein | |||
| | |||
Subunits of [[:Category:Complex:RNA polymerase sigma 19|RNA polymerase sigma 19]] | |||
| | |||
could be indirect | |||
| | |||
| | |||
|- | |||
| | |||
Protein | |||
| | |||
Subunits of [[:Category:Complex:RNA polymerase sigma 28|RNA polymerase sigma 28]] | |||
| | |||
could be indirect | |||
| | |||
| | |||
|- class="tableEdit_footer" | |||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.27049.I46aade4f0f900&page=27049&pagename={{FULLPAGENAMEE}}&type=&template=Product_interactions_table edit table]</span> || || || || | |||
|} | |||
<!--box uid=421aa90e079fa326b6494f812ad13e79.27049.I46aade4f0f900--> | |||
<!--Function_notes--></protect> | |||
===Notes=== | |||
==Localization== | |||
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****************************************************************************************** | |||
* | |||
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | ||
* | * | ||
****************************************************************************************** -->{{Product_resources_help}} | ****************************************************************************************** -->{{Product_localization_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="C46a8701788a40" class=" tableEdit Product_localization_table" | ||
|- {{table_heading_style}} | |- {{table heading style}} | ||
!|Resource type!!Source!!Notes/Reference | !|Compartment!!Description!!Evidence!!Reference/Source!!Notes | ||
|- class="tableEdit_footer" | |- class="tableEdit_footer" | ||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.27049.O46aade5a38f32&page=27049&pagename={{FULLPAGENAMEE}}&type=&template=Product_resource_table edit table]</span> || || | |<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.27049.C46a8701788a40&page=27049&pagename={{FULLPAGENAMEE}}&type=&template=Product_localization_table edit table]</span> || || || || | ||
|} | |} | ||
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<protect><!--Resources_notes--></protect> | <protect><!--Localization_notes--></protect> | ||
===Notes=== | |||
===Notes=== | |||
==Accessions in Other Databases== | |||
{{BacktoTop}} | ==Structure and Physical Properties== | ||
<!--box uid=421aa90e079fa326b6494f812ad13e79.27049.A46aade5a9bcb3--> | {{BacktoTop}} | ||
<!-- | <protect> | ||
****************************************************************************************** | <span id="Sequence"></span><big>'''Physical Properties'''</big> | ||
* | <!--box uid=421aa90e079fa326b6494f812ad13e79.27049.A46aade5718767--> | ||
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | <!-- | ||
* | ****************************************************************************************** | ||
****************************************************************************************** -->{{Gene_accessions_help}} | * | ||
{| | * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | ||
|- {{table_heading_style}} | * | ||
!|Database!!Accession!!Notes | ****************************************************************************************** -->{{Product_physical_properties_help}} | ||
{| id="A46aade5718767" class=" tableEdit Product_phys_prop_table" | |||
|- | |||
!align=left {{table heading style}} |Name | |||
|| | |||
|- | |||
!align=left {{table heading style}} |Sequence | |||
|| | |||
[http://biocyc.org/ECOLI/sequence-aa?object=EG10893-MONOMER at EcoCyc] | |||
<pre>MQGSVTEFLK PRLVDIEQVS STHAKVTLEP LERGFGHTLG NALRRILLSS MPGCAVTEVE | |||
IDGVLHEYST KEGVQEDILE ILLNLKGLAV RVQGKDEVIL TLNKSGIGPV TAADITHDGD | |||
VEIVKPQHVI CHLTDENASI SMRIKVQRGR GYVPASTRIH SEEDERPIGR LLVDACYSPV | |||
ERIAYNVEAA RVEQRTDLDK LVIEMETNGT IDPEEAIRRA ATILAEQLEA FVDLRDVRQP | |||
EVKEEKPEFD PILLRPVDDL ELTVRSANCL KAEAIHYIGD LVQRTEVELL KTPNLGKKSL | |||
TEIKDVLASR GLSLGMRLEN WPPASIADE</pre> | |||
|- | |||
!align=left {{table heading style}} |Length | |||
|| | |||
329 | |||
|- | |||
!align=left {{table heading style}} |Mol. Wt | |||
|| | |||
36.511 kDa | |||
|- | |||
!align=left {{table heading style}} |pI | |||
|| | |||
4.8 (calculated) | |||
|- | |||
!align=left {{table heading style}} |Extinction coefficient | |||
|| | |||
12,950 - 13,450 (calc based on 5 Y, 1 W, and 4 C residues) | |||
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|} | |||
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<span id="Domains/Motifs/Modification Sites"><big>'''Domains/Motifs/Modification Sites'''</big></span><table> | |||
<tr align="left" valign="top"> | |||
<td><!--box uid=421aa90e079fa326b6494f812ad13e79.27049.V46aade578bac2--> | |||
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|- {{table heading style}} | |||
!|Type!!Residues!!Description!!Notes!!References | |||
|- | |||
| | |||
Domain | |||
| | |||
58..178 | |||
| | |||
[http://pfam.janelia.org/family/PF01000 PF01000 RNA polymerase Rpb3/RpoA insert domain] | |||
| | |||
| | |||
PMID:19920124 | |||
|- | |||
| | |||
Domain | |||
| | |||
243..309 | |||
| | |||
[http://pfam.janelia.org/family/PF03118 PF03118 Bacterial RNA polymerase, alpha chain C terminal domain] | |||
| | |||
| | |||
PMID:19920124 | |||
|- | |||
| | |||
Domain | |||
| | |||
28..233 | |||
| | |||
[http://pfam.janelia.org/family/PF01193 PF01193 RNA polymerase Rpb3/Rpb11 dimerisation domain] | |||
| | |||
| | |||
PMID:19920124 | |||
|- class="tableEdit_footer" | |||
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|} | |||
<!--box uid=421aa90e079fa326b6494f812ad13e79.27049.V46aade578bac2--></td> | |||
<td> | |||
<!-- motifs column 2--> | |||
<motif_map/> | |||
</td> | |||
</tr> | |||
</table> | |||
<table><tr valign='top'><td> | |||
<span id='Structure'></span><big>'''Structure'''</big><br /> | |||
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{| id="R46aade57ed578" class=" tableEdit Product_structure_table" | |||
|- | |||
!align=left {{table heading style}} |Structures | |||
|| | |||
<beststructure> | |||
gene=rpoA | |||
taxon=562,83333 | |||
</beststructure> | |||
|- | |||
!align=left {{table heading style}} |Models | |||
|| | |||
View models at: | |||
* [http://www.proteinmodelportal.org/query/uniprot/P0A7Z4 Protein Model Portal] | |||
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P0A7Z4 PSI-PRED (P0A7Z4)] | |||
* [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P0A7Z4 Genomes TO Protein (P0A7Z4)] (GTOP) | |||
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|} | |||
<!--box uid=421aa90e079fa326b6494f812ad13e79.27049.R46aade57ed578--> | |||
</td><td></protect> | |||
==== Structure figures ==== | |||
<protect><!-- ***** PLEASE DO NOT EDIT BELOW THIS LINE ***** --> | |||
</td><tr><table><!--Structure_notes--></protect> | |||
===Notes=== | |||
==Gene Product Resources== | |||
{{BacktoTop}} | |||
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|- {{table_heading_style}} | |||
!|Resource type!!Source!!Notes/Reference | |||
|- class="tableEdit_footer" | |||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.27049.O46aade5a38f32&page=27049&pagename={{FULLPAGENAMEE}}&type=&template=Product_resource_table edit table]</span> || || | |||
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<protect><!--Resources_notes--></protect> | |||
===Notes=== | |||
==Accessions in Other Databases== | |||
{{BacktoTop}} | |||
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="A46aade5a9bcb3" class=" tableEdit Gene_accessions_table" | |||
|- {{table_heading_style}} | |||
!|Database!!Accession!!Notes | |||
|- | |||
| | |||
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]]) | |||
| | |||
[http://www.ncbi.nlm.nih.gov/protein/16131174 GI:16131174] | |||
| | |||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |||
| | |||
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]]) | |||
| | |||
[http://www.ncbi.nlm.nih.gov/protein/NP_417754.1 GeneID:947794] | |||
| | |||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |||
| | |||
[[ASAP]] | |||
| | |||
ASAP:ABE-0010802 | |||
| | |||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |||
| | |||
[http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]]) | |||
| | |||
[http://www.uniprot.org/uniprot/P0A7Z4 UniProtKB/Swiss-Prot:P0A7Z4] | |||
| | |||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |||
| | |||
[[EcoCyc]] | |||
| | |||
EcoCyc:EG10893 | |||
| | |||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[EcoGene]] | |||
| | | | ||
EcoGene:EG10893 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[http:// | [http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]]) | ||
| | | | ||
[ | [http://www.ncbi.nlm.nih.gov/gene?term=947794 GeneID:947794] | ||
| | | | ||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
| | | | ||
[[RegulonDB]] | |||
| | | | ||
RegulonDB:ECK120000884 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[EchoBASE]] | |||
| | | | ||
EchoBASE:EB0886 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[Category:Proteins]] | [[Category:Proteins]] | ||
Latest revision as of 21:16, 15 May 2019
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
RpoA |
|---|---|
| Synonyms |
RNA polymerase, alpha subunit[1], B3295[2][1], Sez[2][1], Phs[2][1], Pez[2][1], RpoA[2][1] , ECK3282, JW3257, pez, phs, sez, b3295 |
| Product description |
RNA polymerase, α subunit[2][3]; Component of RNA polymerase, core enzyme[2][3]; RNA polymerase sigma 24[2][3]; RNA polymerase sigma 38[2][3]; RNA polymerase sigma32[2]; RNA polymerase sigma 32[3]; RNA polymerase sigma54[2]; RNA polymerase sigma 54[3]; RNA polymerase sigma70[2]; RNA polymerase sigma 70[3]; RNA polymerase sigma 19[3]; RNA polymerase sigma 28[3] RNA polymerase, alpha subunit; binds Zn(II)[4] |
| EC number (for enzymes) | |
| edit table |
Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0003677 |
DNA binding |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003899 |
DNA-directed RNA polymerase activity |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003899 |
DNA-directed RNA polymerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003899 |
DNA-directed RNA polymerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003899 |
DNA-directed RNA polymerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003899 |
DNA-directed RNA polymerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
|
Deprecated |
GO:0008270 |
zinc ion binding |
IDA: Inferred from Direct Assay |
F |
The PMID listed is for a paper on spinal cord injury. I cannot find any experimental evidence for Zn binding by the alpha subunit of RNA polymerase. Siegele (talk) 16:16, 15 May 2019 (CDT) |
complete | ||
| GO:0003899 |
DNA-directed RNA polymerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003899 |
DNA-directed RNA polymerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003899 |
DNA-directed RNA polymerase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003899 |
DNA-directed RNA polymerase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006350 |
transcription |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006350 |
transcription |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006350 |
transcription |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006350 |
transcription |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006350 |
transcription |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006350 |
transcription |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006351 |
transcription, DNA-dependent |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006351 |
transcription, DNA-dependent |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016020 |
membrane |
IDA: Inferred from Direct Assay |
C |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0016740 |
transferase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016779 |
nucleotidyltransferase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0046983 |
protein dimerization activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0046983 |
protein dimerization activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of RNA polymerase, core enzyme |
could be indirect |
||
|
Protein |
aceE |
Experiment(s):EBI-879354 | ||
|
Protein |
aceF |
Experiment(s):EBI-879354 | ||
|
Protein |
aspS |
Experiment(s):EBI-879354, EBI-883271 | ||
|
Protein |
mreB |
Experiment(s):EBI-879354 | ||
|
Protein |
pssA |
Experiment(s):EBI-879354 | ||
|
Protein |
waaU |
Experiment(s):EBI-879354 | ||
|
Protein |
rho |
Experiment(s):EBI-879354 | ||
|
Protein |
rplA |
Experiment(s):EBI-879354 | ||
|
Protein |
rplC |
Experiment(s):EBI-879354 | ||
|
Protein |
rplD |
Experiment(s):EBI-879354, EBI-883271 | ||
|
Protein |
rplE |
Experiment(s):EBI-879354 | ||
|
Protein |
rplM |
Experiment(s):EBI-879354, EBI-883271 | ||
|
Protein |
rplO |
Experiment(s):EBI-879354 | ||
|
Protein |
rpsA |
Experiment(s):EBI-879354 | ||
|
Protein |
rpsB |
Experiment(s):EBI-879354 | ||
|
Protein |
rpsC |
Experiment(s):EBI-879354 | ||
|
Protein |
rpsD |
Experiment(s):EBI-879354 | ||
|
Protein |
rpsE |
Experiment(s):EBI-879354, EBI-883271 | ||
|
Protein |
rpsG |
Experiment(s):EBI-879354 | ||
|
Protein |
rpsJ |
Experiment(s):EBI-879354 | ||
|
Protein |
rpsM |
Experiment(s):EBI-879354 | ||
|
Protein |
rplB |
Experiment(s):EBI-879354 | ||
|
Protein |
cspC |
Experiment(s):EBI-883271 | ||
|
Protein |
rplL |
Experiment(s):EBI-883271 | ||
|
Protein |
rplP |
Experiment(s):EBI-883271 | ||
|
Protein |
rplS |
Experiment(s):EBI-883271 | ||
|
Protein |
rplU |
Experiment(s):EBI-883271 | ||
|
Protein |
rplW |
Experiment(s):EBI-883271 | ||
|
Protein |
rplX |
Experiment(s):EBI-883271 | ||
|
Protein |
rpmA |
Experiment(s):EBI-883271 | ||
|
Protein |
rpmB |
Experiment(s):EBI-883271 | ||
|
Protein |
rpmG |
Experiment(s):EBI-883271 | ||
|
Protein |
rpsN |
Experiment(s):EBI-883271 | ||
|
Protein |
rpsO |
Experiment(s):EBI-883271 | ||
|
Protein |
rpsF |
Experiment(s):EBI-883271 | ||
|
Protein |
rpsP |
Experiment(s):EBI-883271 | ||
|
Protein |
rpsS |
Experiment(s):EBI-883271 | ||
|
Protein |
rpsT |
Experiment(s):EBI-883271 | ||
|
Protein |
rpsU |
Experiment(s):EBI-883271 | ||
|
Protein |
wecG |
Experiment(s):EBI-883271 | ||
|
Protein |
rplB |
Experiment(s):EBI-1145418 | ||
|
Protein |
rpsD |
Experiment(s):EBI-1145418 | ||
|
Protein |
hybE |
Experiment(s):EBI-1145418 | ||
|
Protein |
rpsE |
Experiment(s):EBI-1145418 | ||
|
Protein |
pta |
Experiment(s):EBI-1145418 | ||
|
Protein |
etk |
Experiment(s):EBI-1145418 | ||
|
Protein |
rpoB |
Experiment(s):EBI-1145418 | ||
|
Protein |
greA |
Experiment(s):EBI-1145418 | ||
|
Protein |
rplO |
Experiment(s):EBI-1145418 | ||
|
Protein |
rplF |
Experiment(s):EBI-1145418 | ||
|
Protein |
rapA |
Experiment(s):EBI-1145418 | ||
|
Protein |
rpoD |
Experiment(s):EBI-1145418 | ||
|
Protein |
fur |
Experiment(s):EBI-1145418 | ||
|
Protein |
mreB |
MALDI(Z-score):36.959637 | ||
|
Protein |
rpsP |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpsS |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplU |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpsT |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpsN |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplP |
LCMS(ID Probability):99.6 MALDI(Z-score):3.353874 | ||
|
Protein |
rplX |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpmG |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpmA |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpsA |
MALDI(Z-score):21.278856 | ||
|
Protein |
rpmB |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplA |
MALDI(Z-score):32.556213 | ||
|
Protein |
rplS |
LCMS(ID Probability):99.6 | ||
|
Protein |
aspS |
LCMS(ID Probability):99.6 MALDI(Z-score):8.467531 | ||
|
Protein |
rpsJ |
LCMS(ID Probability):97.9 MALDI(Z-score):16.475126 | ||
|
Protein |
rpsF |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplW |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplM |
LCMS(ID Probability):99.6 MALDI(Z-score):17.053160 | ||
|
Protein |
hupA |
LCMS(ID Probability):99.6 MALDI(Z-score):9.189812 | ||
|
Protein |
rpsC |
MALDI(Z-score):41.404786 | ||
|
Protein |
rplB |
MALDI(Z-score):37.610726 | ||
|
Protein |
rpsO |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpsD |
MALDI(Z-score):34.513588 | ||
|
Protein |
rho |
MALDI(Z-score):37.049801 | ||
|
Protein |
cspC |
LCMS(ID Probability):99.6 MALDI(Z-score):13.185220 | ||
|
Protein |
rplD |
LCMS(ID Probability):99.6 MALDI(Z-score):27.436806 | ||
|
Protein |
greA |
LCMS(ID Probability):93.1 MALDI(Z-score):17.053160 | ||
|
Protein |
rpsU |
LCMS(ID Probability):99.6 | ||
|
Protein |
pssA |
MALDI(Z-score):19.670165 | ||
|
Protein |
nusG |
LCMS(ID Probability):99.6 MALDI(Z-score):35.117471 | ||
|
Protein |
rpoB |
LCMS(ID Probability):99.6 MALDI(Z-score):39.950272 | ||
|
Protein |
rpoC |
LCMS(ID Probability):99.6 MALDI(Z-score):39.702103 | ||
|
Protein |
rapA |
LCMS(ID Probability):99.6 MALDI(Z-score):18.240902 | ||
|
Protein |
rpoZ |
LCMS(ID Probability):99.6 MALDI(Z-score):38.277175 | ||
|
Protein |
rffM |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpoD |
LCMS(ID Probability):99.6 MALDI(Z-score):40.069162 | ||
|
Protein |
rplL |
LCMS(ID Probability):99.6 | ||
|
Protein |
Subunits of RNA polymerase sigma 24 |
could be indirect |
||
|
Protein |
Subunits of RNA polymerase sigma 38 |
could be indirect |
||
|
Protein |
Subunits of RNA polymerase sigma32 |
could be indirect |
||
|
Protein |
Subunits of RNA polymerase sigma54 |
could be indirect |
||
|
Protein |
Subunits of RNA polymerase sigma70 |
could be indirect |
||
|
Protein |
Subunits of RNA polymerase sigma 19 |
could be indirect |
||
|
Protein |
Subunits of RNA polymerase sigma 28 |
could be indirect |
| |
| edit table |
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
| edit table |
Notes
Structure and Physical Properties
Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MQGSVTEFLK PRLVDIEQVS STHAKVTLEP LERGFGHTLG NALRRILLSS MPGCAVTEVE IDGVLHEYST KEGVQEDILE ILLNLKGLAV RVQGKDEVIL TLNKSGIGPV TAADITHDGD VEIVKPQHVI CHLTDENASI SMRIKVQRGR GYVPASTRIH SEEDERPIGR LLVDACYSPV ERIAYNVEAA RVEQRTDLDK LVIEMETNGT IDPEEAIRRA ATILAEQLEA FVDLRDVRQP EVKEEKPEFD PILLRPVDDL ELTVRSANCL KAEAIHYIGD LVQRTEVELL KTPNLGKKSL TEIKDVLASR GLSLGMRLEN WPPASIADE |
| Length |
329 |
| Mol. Wt |
36.511 kDa |
| pI |
4.8 (calculated) |
| Extinction coefficient |
12,950 - 13,450 (calc based on 5 Y, 1 W, and 4 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
|
Structure figures
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ Groomes, TE & Huang, CT (1991) Orthostatic hypotension after spinal cord injury: treatment with fludrocortisone and ergotamine. Arch Phys Med Rehabil 72 56-8 PubMed
- ↑ Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
- ↑ 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 7.11 7.12 7.13 7.14 7.15 7.16 7.17 7.18 7.19 7.20 7.21 7.22 7.23 7.24 7.25 7.26 7.27 7.28 7.29 7.30 7.31 7.32 7.33 7.34 7.35 7.36 7.37 7.38 7.39 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
- ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 8.11 8.12 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
- ↑ 9.00 9.01 9.02 9.03 9.04 9.05 9.06 9.07 9.08 9.09 9.10 9.11 9.12 9.13 9.14 9.15 9.16 9.17 9.18 9.19 9.20 9.21 9.22 9.23 9.24 9.25 9.26 9.27 9.28 9.29 9.30 9.31 9.32 9.33 9.34 9.35 9.36 9.37 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
- ↑ 10.0 10.1 10.2 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0003677 ! DNA binding
- GO:0003899 ! DNA-directed 5'-3' RNA polymerase activity
- GO:0008270 ! zinc ion binding
- GO:0006350 ! transcription
- GO:0006351 ! transcription, DNA-templated
- GO:0016020 ! membrane
- GO:0016740 ! transferase activity
- GO:0016779 ! nucleotidyltransferase activity
- GO:0046983 ! protein dimerization activity
- Proteins