adhE:Expression: Difference between revisions
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== | ==Cellular Levels== | ||
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****************************************************************************************** --> | ****************************************************************************************** -->{{Help Cellular levels}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="F49c01b500b12c" class=" tableEdit MoleculesPerCellTable" | ||
|- align='left' bgcolor='#CCCCFF' | |- align='left' bgcolor='#CCCCFF' | ||
!|Molecule!!Organism or Strain!! | !|Molecule!!Organism or Strain!!Value!!Units!!Experimental Conditions!!Assay used!!Notes!!Reference(s) | ||
|- | |||
| | |||
Protein | |||
| | |||
''E. coli'' K-12 MC4100 | |||
| | |||
5.43E+03 | |||
| | |||
molecules/cell | |||
| | |||
*Medium: median data from rich and minimal media | |||
*Temperature (°C): 37 | |||
*Doubling time (min): Growth Rate(min): | |||
*Growth phase: other: | |||
| | |||
emPAI | |||
| | |||
| | |||
PMID:18304323 | |||
|- | |||
| | |||
Protein | |||
| | |||
''E. coli'' K-12 MG1655 | |||
| | |||
3485 | |||
| | |||
molecules/cell/generation | |||
| | |||
*Medium: MOPS Complete | |||
* Temperature (°C): 37 | |||
*Doubling time (min): | |||
* *Growth phase: | |||
*OD: | |||
*other: | |||
| | |||
Ribosome Profiling | |||
| | |||
| | |||
PMID: 24766808 | |||
|- | |||
| | |||
Protein | |||
| | |||
''E. coli'' K-12 MG1655 | |||
| | |||
4687 | |||
| | |||
molecules/cell/generation | |||
| | |||
*Medium: MOPS Minimal | |||
* Temperature (°C): 37 | |||
*Doubling time (min): | |||
* *Growth phase: | |||
*OD: | |||
*other: | |||
| | |||
Ribosome Profiling | |||
| | |||
| | |||
PMID: 24766808 | |||
|- | |||
| | |||
Protein | |||
| | |||
''E. coli'' K-12 MG1655 | |||
| | |||
3197 | |||
| | |||
molecules/cell/generation | |||
| | |||
*Medium: MOPS Complete without Methionine | |||
* Temperature (°C): 37 | |||
*Doubling time (min): | |||
* *Growth phase: | |||
*OD: | |||
*other: | |||
| | |||
Ribosome Profiling | |||
| | |||
| | |||
PMID: 24766808 | |||
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****************************************************************************************** -->{{expression_transcription_help}} | ****************************************************************************************** -->{{expression_transcription_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="T46ab4dd896b74" class=" tableEdit Expression_operons_table" | ||
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!|Transcription unit(s) | !|Transcription unit(s) | ||
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[[:Category:Txn_unit:adhE|adhE]] | [[:Category:Txn_unit:adhE|adhE]] | ||
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==Translation and Regulation of Translation== | ==Translation and Regulation of Translation== | ||
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<protect> | <protect><gbrowseImage> | ||
name=NC_000913:1297324..1297364 | |||
source=MG1655 | |||
flip=1 | |||
type=Gene+DNA_+Protein | |||
preset=Nterminus | |||
</gbrowseImage> | |||
{{Nterm_closeup}} | |||
<!--Translation_notes--></protect> | <!--Translation_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
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!|Allele Name!!Mutation!!Phenotype!!Reference | !|Allele Name!!Mutation!!Phenotype!!Reference | ||
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!|Type!!Reference!!Notes | !|Type!!Reference!!Notes | ||
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microarray | microarray | ||
| | | | ||
[http://genexpdb.ou.edu/index.php?query=b1241 GenExpDB:b1241] ([[EcoliGenExpDB|EcoliWiki Page]]) | [http://genexpdb.ou.edu/index.php?query=b1241 GenExpDB:b1241] ([[EcoliGenExpDB|EcoliWiki Page]]) | ||
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Summary of data for adhE from multiple microarray studies | Summary of data for adhE from multiple microarray studies | ||
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!|Resource Name!!Resource Type!!Description!!Source!!Notes | !|Resource Name!!Resource Type!!Description!!Source!!Notes | ||
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GFP fusion described in Zaslaver, <i>et al.</i><br />Plate:109-AZ04; Well:B3<ref name="PMID: 16862137" /> | GFP fusion described in Zaslaver, <i>et al.</i><br />Plate:109-AZ04; Well:B3<ref name="PMID: 16862137" /> | ||
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!|Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
|- | |- | ||
| | | | ||
[[EcoCyc]] | |||
| | | | ||
EcoCyc:EG10031 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[EchoBASE]] | |||
| | | | ||
EchoBASE:EB0030 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[ | [[EcoGene]] | ||
| | |||
EcoGene:EG10031 | |||
| | |||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |||
| | |||
[[RegulonDB]] | |||
| | |||
RegulonDB:ECK120000028 | |||
| | |||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |||
| | | | ||
[ | [[ASAP]] | ||
| | | | ||
ASAP:ABE-0004164 | |||
| | |||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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==References== | ==References== | ||
{{BacktoTop}} | {{BacktoTop}} | ||
{{RefHelp}} | |||
<references/> | <references /> | ||
__NOTOC__ | __NOTOC__ | ||
===Categories=== | ===Categories=== | ||
{{BacktoTop}} | {{BacktoTop}} | ||
[[Category:Txn_unit:adhE]] | [[Category:Txn_unit:adhE]] | ||
Latest revision as of 19:54, 21 July 2015
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Overview | Transcription | Translation | Turnover | Experimental | Links | References | Suggestions |
Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
| Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
|---|---|---|---|---|---|---|---|
|
Protein |
E. coli K-12 MC4100 |
5.43E+03 |
molecules/cell |
|
emPAI |
||
|
Protein |
E. coli K-12 MG1655 |
3485 |
molecules/cell/generation |
|
Ribosome Profiling |
||
|
Protein |
E. coli K-12 MG1655 |
4687 |
molecules/cell/generation |
|
Ribosome Profiling |
||
|
Protein |
E. coli K-12 MG1655 |
3197 |
molecules/cell/generation |
|
Ribosome Profiling |
||
| edit table |
Notes
Transcription and Transcriptional Regulation
|
See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<gbrowseImage> name=NC_000913:1297324..1297364 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.
Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
</protect>
Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
| Allele Name | Mutation | Phenotype | Reference |
|---|---|---|---|
| edit table |
Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
| Type | Reference | Notes |
|---|---|---|
|
microarray |
NCBI GEO profiles for adhE | |
|
microarray |
Summary of data for adhE from multiple microarray studies | |
| edit table |
Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
| Resource Name | Resource Type | Description | Source | Notes |
|---|---|---|---|---|
|
GFP Fusion |
Intergenic region (1297274..1297865) fused to gfpmut2. |
GFP fusion described in Zaslaver, et al. | ||
| edit table |
<protect>
Notes
Accessions Related to adhE Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Ishihama, Y et al. (2008) Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics 9 102 PubMed
- ↑ 2.0 2.1 2.2 Li, GW et al. (2014) Quantifying absolute protein synthesis rates reveals principles underlying allocation of cellular resources. Cell 157 624-35 PubMed
- ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed
Categories
